FastQCFastQC Report
Wed 8 May 2024
EGAF00008120118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115378
Total Bases34.7 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG25462.2066598484979805No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG22391.940577926467784No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG18811.630293470158956No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG18001.5600894451281873No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG14451.2524051378945726No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG14171.2281370798592453No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG14151.2264036471424362No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG10930.9473209797361715No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG9960.8632494929709303No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG6160.5338972767772019No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG5190.44982579001196066No Hit
GNNTANCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4710.40822340480854236No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4630.40128967394130594No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4340.37615489954757403No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4290.3718213177555513No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4140.3588205723794831No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4000.3466865433618194No Hit
GNNTANTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3920.339752812494583No Hit
GNNTANCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3590.3111511726672329No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3550.30768430723361473No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3510.30421744179999655No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3470.30075057636637836No Hit
NANTNNTGGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTCCTCAG3330.28861654734871467No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3280.2842829655566919No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3220.27908266740626464No Hit
GNNTANCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3220.27908266740626464No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3090.2678153547470055No Hit
GNNTANAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3070.26608192203019637No Hit
GANTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3050.2643484893133873No Hit
NANTNNTCGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTCCTCAG3050.2643484893133873No Hit
NNCTNNTGGGGTATNTANTCNTGTTCGNTCNCCNTGCTTTCGCTCCTCAG3000.2600149075213646No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2910.25221446029572364No Hit
GACTACTGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG2890.2504810275789145No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2890.2504810275789145No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2880.24961431122051No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2860.24788087850370089No Hit
GANTANTGGGGTNTCTNNTNNTGTTCGCTCCCCATGNTTTCGCTCCTCAG2820.2444140130700827No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2810.24354729671167813No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2790.241813863994869No Hit
NANTNNCGGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTCCTCAG2710.23488013312763265No Hit
NANTANTGGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG2690.23314670041082358No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2640.22881311861880083No Hit
GNNTANTGGGGTNTNTNNTNNTGTTCGNTCNCNNTGNTTTCGCTCCTNNG2640.22881311861880083No Hit
NNNTNNTGGGGTATNTNNTNNTGTTCGNTCNCCNTGNTTTCGCTCCTCAG2620.2270796859019917No Hit
GNNTANACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2590.22447953682677807No Hit
GANTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2590.22447953682677807No Hit
NNNTNNTGGGNNNTNTNATNNTGTTCGNTCNCCNTGNTTTCGCTCCNNNG2580.22361282046837352No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2540.22014595503475534No Hit
GANTNNTGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG2530.21927923867635077No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2530.21927923867635077No Hit
NNCTNNTCGGGTATNTANTCNTGTTCGNTCNCCNTGCTTTCGCTCCTCAG2530.21927923867635077No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2500.21667908960113716No Hit
GNNTANAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2490.21581237324273259No Hit
NNNTNNTCGGGTATNTNNTNNTGTTCGNTCNCCNTGNTTTCGCTCCTCAG2480.21494565688432804No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2450.2123455078091144No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2440.21147879145070986No Hit
NNNTNNTCGGNNNTNTNATNNTGTTCGNTCNCCNTGNTTTCGCTCCNNNG2430.21061207509230528No Hit
NNNTNNCGGGNNNTNTNATNNTGTTCGNTCNCCNTGNTTTCGCTCCNNNG2390.2071452096586871No Hit
GNNTANTCGGGTNTNTNNTNNTGTTCGNTCNCNNTGNTTTCGCTCCTNNG2350.20367834422506892No Hit
GNNTANTAGGGTNTNTNNTNNTGTTCGNTCNCNNTGNTTTCGCTCCTNNG2320.20107819514985523No Hit
GANTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2320.20107819514985523No Hit
NANTNNTAGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTCCTCAG2300.19934476243304616No Hit
NNNTNNCGGGGTATNTNNTNNTGTTCGNTCNCCNTGNTTTCGCTCCTCAG2300.19934476243304616No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2250.1950111806410234No Hit
GANTNNCGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG2230.19327774792421432No Hit
GACTACTCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG2230.19327774792421432No Hit
NANTANTCGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG2220.19241103156580977No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2220.19241103156580977No Hit
NNNTNNTAGGGTATNTNNTNNTGTTCGNTCNCCNTGNTTTCGCTCCTCAG2200.19067759884900068No Hit
GANTANTCGGGTNTCTNNTNNTGTTCGCTCCCCATGNTTTCGCTCCTCAG2180.1889441661321916No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2180.1889441661321916No Hit
GANTNNTAGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG2160.1872107334153825No Hit
GANTANCGGGGTNTCTNNTNNTGTTCGCTCCCCATGNTTTCGCTCCTCAG2160.1872107334153825No Hit
GANTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2140.1854773006985734No Hit
GANTNNTCGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG2110.18287715162335974No Hit
NANTANCGGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG2040.1768101371145279No Hit
GANTNNCCGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG2020.1750767043977188No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2000.1733432716809097No Hit
NNCTNNCGGGGTATNTANTCNTGTTCGNTCNCCNTGCTTTCGCTCCTCAG2000.1733432716809097No Hit
GANTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2000.1733432716809097No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1990.17247655532250517No Hit
GNNTANCGGGGTNTNTNNTNNTGTTCGNTCNCNNTGNTTTCGCTCCTNNG1960.1698764062472915No Hit
GACTACCGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1960.1698764062472915No Hit
NNNTNNTAGGNNNTNTNATNNTGTTCGNTCNCCNTGNTTTCGCTCCNNNG1950.16900968988888695No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1950.16900968988888695No Hit
GANTANTAGGGTNTCTNNTNNTGTTCGCTCCCCATGNTTTCGCTCCTCAG1930.16727625717207786No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1890.16380939173845965No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1860.16120924266324604No Hit
NNCTNNCAGGGTATNTANTCNTGTTCGNTCNCCNTGCTTTCGCTCCTCAG1840.15947580994643692No Hit
NANTNNAGGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTCCTCAG1840.15947580994643692No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1830.15860909358803238No Hit
NANTNNCAGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTCCTCAG1830.15860909358803238No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1830.15860909358803238No Hit
NNNTNNCAGGGTATNTNNTNNTGTTCGNTCNCCNTGNTTTCGCTCCTCAG1820.15774237722962783No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1810.1568756608712233No Hit
NANTNNCCGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTCCTCAG1790.1551422281544142No Hit
NANTANTAGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG1770.15340879543760508No Hit
GACTACTAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1760.15254207907920053No Hit
NNNTNNCAGGNNNTNTNATNNTGTTCGNTCNCCNTGNTTTCGCTCCNNNG1730.1499419300039869No Hit
GANTNNCAGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG1720.14907521364558235No Hit
NNCTNNTAGGGTATNTANTCNTGTTCGNTCNCCNTGCTTTCGCTCCTCAG1720.14907521364558235No Hit
GANTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1720.14907521364558235No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1680.14560834821196414No Hit
NANTANAGGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG1670.14474163185355962No Hit
GACTACAGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1660.14387491549515505No Hit
NNNTNNCCGGGTATNTNNTNNTGTTCGNTCNCCNTGNTTTCGCTCCTCAG1660.14387491549515505No Hit
GACTACCCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1660.14387491549515505No Hit
NNCTNNAGGGGTATNTANTCNTGTTCGNTCNCCNTGCTTTCGCTCCTCAG1620.14040805006153687No Hit
GANTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1610.13954133370313232No Hit
GANTANCCGGGTNTCTNNTNNTGTTCGCTCCCCATGNTTTCGCTCCTCAG1580.13694118462791868No Hit
NNNTNNAGGGNNNTNTNATNNTGTTCGNTCNCCNTGNTTTCGCTCCNNNG1560.13520775191110956No Hit
NANTANCAGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG1560.13520775191110956No Hit
NANTNNACGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTCCTCAG1530.13260760283589593No Hit
NNNTNNCCGGNNNTNTNATNNTGTTCGNTCNCCNTGNTTTCGCTCCNNNG1520.13174088647749138No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1490.12914073740227772No Hit
GANTANCAGGGTNTCTNNTNNTGTTCGCTCCCCATGNTTTCGCTCCTCAG1480.1282740210438732No Hit
NANTANCCGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG1470.12740730468546863No Hit
GACTACCAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1460.12654058832706408No Hit
NNNTNNAGGGGTATNTNNTNNTGTTCGNTCNCCNTGNTTTCGCTCCTCAG1460.12654058832706408No Hit
NNCTNNCCGGGTATNTANTCNTGTTCGNTCNCCNTGCTTTCGCTCCTCAG1460.12654058832706408No Hit
NANTNNAAGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTCCTCAG1460.12654058832706408No Hit
GNNTANCAGGGTNTNTNNTNNTGTTCGNTCNCNNTGNTTTCGCTCCTNNG1450.12567387196865953No Hit
GNNTANCCGGGTNTNTNNTNNTGTTCGNTCNCNNTGNTTTCGCTCCTNNG1450.12567387196865953No Hit
GNNTANAGGGGTNTNTNNTNNTGTTCGNTCNCNNTGNTTTCGCTCCTNNG1410.12220700653504135No Hit
GANTANAGGGGTNTCTNNTNNTGTTCGCTCCCCATGNTTTCGCTCCTCAG1400.12134029017663679No Hit
GANTNNAGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG1390.12047357381823225No Hit
GACTACACGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1260.10920626115897311No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1240.10747282844216402No Hit
GANTANACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1240.10747282844216402No Hit
NNCTNNACGGGTATNTANTCNTGTTCGNTCNCCNTGCTTTCGCTCCTCAG1230.10660611208375947No Hit
NNCTNNAAGGGTATNTANTCNTGTTCGNTCNCCNTGCTTTCGCTCCTCAG1230.10660611208375947No Hit
GANTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1220.10573939572535493No Hit
GANTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1210.10487267936695037No Hit
GACTACAAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1210.10487267936695037No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)