FastQCFastQC Report
Tue 8 Aug 2023
EGAF00008120123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20689
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3751.8125573976509255No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2911.4065445405771184No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2831.3678766494272319No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2721.314708299096138No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2451.1842041664652714No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2371.145536275315385No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2010.9715307651408961No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1990.9618637923534245No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1900.9183624148098023No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1860.8990284692348591No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1810.8748610372661801No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1720.8313596597225579No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1560.7540238774227851No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1530.7395234182415776No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1470.7105224998791628No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1420.6863550679104838No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1390.6718546087292764No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1380.6670211223355406No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1340.6476871767605974No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1330.6428536903668616No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1310.63318671757939No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1160.560684421673353No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1130.5461839624921456No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1070.5171830441297307No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1050.5075160713422592No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1030.49784909855478754No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT1020.4930156121610517No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1000.48334863937358014No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT990.47851515297984437No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT980.47368166658610855No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC920.44468074822369374No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC910.439847261829958No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC890.43018028904248634No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT840.40601285707380735No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT830.4011793706800716No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT770.3721784523176567No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC770.3721784523176567No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT770.3721784523176567No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT760.3673449659239209No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT750.36251147953018514No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATATGTAGACTTGGGAT750.36251147953018514No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATATGTAGACTTGGGAT730.35284450674271356No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC710.3431775339552419No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT710.3431775339552419No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT680.3286770747740345No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT660.3190101019865629No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATATGTAGACTTGGGAT660.3190101019865629No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT650.3141766155928271No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT640.3093431291990913No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC630.30450964280535553No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC630.30450964280535553No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATATGTAGACTTGGGAT620.2996761564116197No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT600.2900091836241481No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC600.2900091836241481No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC590.2851756972304123No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT590.2851756972304123No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC590.2851756972304123No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT580.2803422108366765No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT570.2755087244429407No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT540.26100826526173326No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATATGTAGACTTGGGAT540.26100826526173326No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATATGTAGACTTGGGAT530.25617477886799744No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT520.2513412924742617No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT520.2513412924742617No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC500.24167431968679007No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC490.23684083329305428No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT470.22717386050558266No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT470.22717386050558266No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT470.22717386050558266No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT450.21750688771811108No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCTTCACGGGAT440.2126734013243753No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTGTCGTAGGAT420.20300642853690368No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATATGTAGACTTGGGAT420.20300642853690368No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTATAAGAGAAGC400.19333945574943207No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT390.18850596935569627No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT390.18850596935569627No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC390.18850596935569627No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT390.18850596935569627No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT390.18850596935569627No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTGTCGTAGGAT380.18367248296196045No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT370.17883899656822466No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT370.17883899656822466No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT370.17883899656822466No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT360.17400551017448887No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTGTCGTAGGAT360.17400551017448887No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC360.17400551017448887No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC350.16917202378075305No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC350.16917202378075305No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT350.16917202378075305No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC340.16433853738701726No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT340.16433853738701726No Hit
CCTACGGGTGGCTGCAGTCGTCGGCCGCGTCAGATGTGTTATAACCGGAT330.15950505099328144No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT330.15950505099328144No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATATGTAGACTTGGGAT310.14983807820580985No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT300.14500459181207406No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC300.14500459181207406No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC300.14500459181207406No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT300.14500459181207406No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC300.14500459181207406No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC290.14017110541833824No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT290.14017110541833824No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT290.14017110541833824No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTAGGAT290.14017110541833824No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTAGGAT280.13533761902460245No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTGTCGTAGGAT280.13533761902460245No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTATAAGAGAAGC270.13050413263086663No Hit
CCTACGGGTGGCAGCAGTCGTCGGCCGCGTCAGATGTGTTATAACCGGAT270.13050413263086663No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT260.12567064623713084No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTAGGAT260.12567064623713084No Hit
CCTACGGGGGGCTGCAGTCGTCGGCCGCGTCAGATGTGTTATAACCGGAT250.12083715984339503No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT250.12083715984339503No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCTTCACGGGAT250.12083715984339503No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTATAAGAGAAGC240.11600367344965924No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC240.11600367344965924No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT240.11600367344965924No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT240.11600367344965924No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT240.11600367344965924No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTAGGAT240.11600367344965924No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT230.11117018705592344No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT230.11117018705592344No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTATAAGAGAAGC230.11117018705592344No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTGTCGTAGGAT230.11117018705592344No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT230.11117018705592344No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC230.11117018705592344No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTATAACAGGAT230.11117018705592344No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTAGGAT230.11117018705592344No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT230.11117018705592344No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC220.10633670066218764No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC220.10633670066218764No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT220.10633670066218764No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT220.10633670066218764No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTGTCGTAGGAT210.10150321426845184No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC210.10150321426845184No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT210.10150321426845184No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTATAACAGGAT210.10150321426845184No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCTTCACGGGAT210.10150321426845184No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTGTCGTAGGAT210.10150321426845184No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)