FastQCFastQC Report
Tue 8 Aug 2023
EGAF00008120124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20689
Sequences flagged as poor quality0
Sequence length301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2991.4452124317270048No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2251.0875344385905554No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2141.0343660882594614No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2091.0101986562907825No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2071.000531683503311No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1940.9376963603847455No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1920.9280293875972739No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1740.8410266325100295No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1740.8410266325100295No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1690.8168592005413505No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1670.8071922277538789No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1640.7926917685726715No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1560.7540238774227851No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1550.7491903910290493No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1530.7395234182415776No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1490.7201894726666345No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1460.7056890134854271No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1400.6766880951230122No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1390.6718546087292764No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1360.657354149548069No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1290.6235197447919184No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1210.584851853642032No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1200.5800183672482963No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1190.5751848808545604No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1140.5510174488858814No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1130.5461839624921456No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1100.5316835033109382No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1090.5268500169172023No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1090.5268500169172023No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1070.5171830441297307No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1070.5171830441297307No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1060.5123495577359949No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1000.48334863937358014No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG980.47368166658610855No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG950.45918120740490115No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA940.45434772101116533No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG930.44951423461742956No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA920.44468074822369374No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACATATCTGACGCTGCCGACGA900.43501377543622216No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA850.4108463434675432No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG850.4108463434675432No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA850.4108463434675432No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACATATCTGACGCTGCCGACGA830.4011793706800716No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG820.39634588428633577No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACATATCTGACGCTGCCGACGA800.38667891149886413No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA750.36251147953018514No Hit
GACTACCAGGGTATCTAATCCCAAGTCTACATATCTGACGCTGCCGACGA750.36251147953018514No Hit
GACTACTGGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA740.3576779931364493No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG730.35284450674271356No Hit
GACTACTCGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA730.35284450674271356No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA730.35284450674271356No Hit
GACTACCGGGGTATCTAATCCCAAGTCTACATATCTGACGCTGCCGACGA710.3431775339552419No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACATATCTGACGCTGCCGACGA690.3335105611677703No Hit
GACTACTAGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA620.2996761564116197No Hit
GACTACCCGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA610.2948426700178839No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG590.2851756972304123No Hit
GACTACACGGGTATCTAATCCCAAGTCTACATATCTGACGCTGCCGACGA580.2803422108366765No Hit
GACTACCAGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA560.2706752380492049No Hit
GACTACAGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA540.26100826526173326No Hit
GACTACCGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA530.25617477886799744No Hit
GACTACCAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA530.25617477886799744No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA530.25617477886799744No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG520.2513412924742617No Hit
GACTACAAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA500.24167431968679007No Hit
GACTACAAGGGTATCTAATCCCAAGTCTACATATCTGACGCTGCCGACGA500.24167431968679007No Hit
GACTACTCGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACGA460.22234037411184687No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA450.21750688771811108No Hit
GACTACTGGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACGA450.21750688771811108No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA440.2126734013243753No Hit
GACTACAGGGGTATCTAATCCCAAGTCTACATATCTGACGCTGCCGACGA440.2126734013243753No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA440.2126734013243753No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG430.20783991493063947No Hit
GACTACAAGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA420.20300642853690368No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG410.19817294214316789No Hit
GACTACCGGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA400.19333945574943207No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA400.19333945574943207No Hit
GACTACTGGGGTATCTAATCCTACGACAACACATCTGACGCTGCCGACGA400.19333945574943207No Hit
GACTACCCGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACGA390.18850596935569627No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA380.18367248296196045No Hit
GACTACTCGGGTATCTAATCCTAAGTCTACACATCTGACGCTGCCGACGA380.18367248296196045No Hit
GACTACAGGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA360.17400551017448887No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG360.17400551017448887No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG340.16433853738701726No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG330.15950505099328144No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG330.15950505099328144No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG330.15950505099328144No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA330.15950505099328144No Hit
GACTACACGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA330.15950505099328144No Hit
GACTACACGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA330.15950505099328144No Hit
GACTACTCGGGTATCTAATCCTACGACAACACATCTGACGCTGCCGACGA320.15467156459954565No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG320.15467156459954565No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA320.15467156459954565No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG310.14983807820580985No Hit
GACTACTAGGGTATCTAATCCTACGACAACACATCTGACGCTGCCGACGA310.14983807820580985No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG310.14983807820580985No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG300.14500459181207406No Hit
GACTACTGGGGTATCTAATCCTGTTATAACACATCTGACGCTGCCGACGA300.14500459181207406No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA290.14017110541833824No Hit
GACTACCAGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACGA290.14017110541833824No Hit
GACTACTGGGGTATCTAATCCTAAGTCTACACATCTGACGCTGCCGACGA270.13050413263086663No Hit
GACTACCCGGGTATCTAATCCTACGACAACACATCTGACGCTGCCGACGA270.13050413263086663No Hit
GACTACAGGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACGA270.13050413263086663No Hit
GACTACTAGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA270.13050413263086663No Hit
GACTACTAGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACGA260.12567064623713084No Hit
GACTACTAGGGTATCTAATCCTAAGTCTACACATCTGACGCTGCCGACGA250.12083715984339503No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA250.12083715984339503No Hit
GACTACTGGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA250.12083715984339503No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG250.12083715984339503No Hit
GACTACAGGGGTATCTAATCCTACGACAACACATCTGACGCTGCCGACGA240.11600367344965924No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG240.11600367344965924No Hit
GACTACTCGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA240.11600367344965924No Hit
GACTACTGGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA240.11600367344965924No Hit
GACTACTCGGGTATCTAATCCGGTTATAACACATCTGACGCGGCCGACGA230.11117018705592344No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA230.11117018705592344No Hit
GACTACTCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC230.11117018705592344No Hit
GACTACTAGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA230.11117018705592344No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG220.10633670066218764No Hit
GACTACTCGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA220.10633670066218764No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG220.10633670066218764No Hit
GACTACCGGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACGA220.10633670066218764No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG210.10150321426845184No Hit
GACTACTGGGGTATCTAATCCTGCAGCCCCCCGTAGGCTGTCTCTTATAA210.10150321426845184No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA210.10150321426845184No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)