FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008120126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91109
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG10121.110757444379809No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG9991.0964888210824397No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG8650.9494122424787891No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG7740.8495318793972054No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7200.7902622133927494No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6800.7463587570931521No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6250.6859915046812061No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG6210.6816011590512463No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5900.6475759804190585No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5780.6344049435291793No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5520.6058676969344412No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5030.5520859629674346No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG4650.5103776794828173No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4630.5081825066678375No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4590.5037921610378777No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4290.4708645688131798No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4050.4445224950334215No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3990.4379369765884819No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3910.4291562853285625No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3790.4159852484386833No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG3730.4093997299937438No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3320.36439868728665664No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG3270.358910755249207No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG3080.3380566135068983No Hit
GNNTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2900.3183000581720796No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG2890.31720247176458966No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2750.30183626205973063No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2750.30183626205973063No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG2730.2996410892447508No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2590.2842748795398918No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG2570.28207970672491195No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2560.280982120317422No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2430.2667134970200529No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2430.2667134970200529No Hit
GNNTANTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2390.2623231513900932No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2310.2535424601301738No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2300.2524448737226838No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG2270.24915211450021402No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2270.24915211450021402No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2150.23598107761033488No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2050.2250052135354356No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2050.2250052135354356No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2030.2228100407204557No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2020.2217124543129658No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1910.20963900383057657No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1850.20305348538563697No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1830.20085831257065714No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1830.20085831257065714No Hit
GNNTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1760.1931752077182276No Hit
GNNTANCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1740.19098003490324775No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1730.18988244849575783No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1730.18988244849575783No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1730.18988244849575783No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG1720.1887848620882679No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1700.18658968927328803No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1680.18439451645830818No Hit
GNNTANCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1670.18329693005081824No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1630.17890658442085852No Hit
GANTANTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1540.16902830675344915No Hit
GNNTANAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1530.16793072034595924No Hit
GNNTANCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1520.1668331339384693No Hit
GNNTANTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1510.16573554753097938No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1430.15695485627105993No Hit
GANTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1410.15475968345608007No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1410.15475968345608007No Hit
NNNTNNTCGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG1410.15475968345608007No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG1400.15366209704859016No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1390.15256451064110021No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1350.1481741650111405No Hit
GNNTANTGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG1340.1470765786036506No Hit
NNNTNNTGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG1330.14597899219616065No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1320.14488140578867073No Hit
NNNTNNTGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG1300.14268623297369085No Hit
GANTNNTGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG1280.140491060158711No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1280.140491060158711No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1270.13939347375122108No Hit
GNNTANTCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG1240.13610071452875128No Hit
GANTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1210.1328079553062815No Hit
GNNTANCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1170.12841760967632176No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1170.12841760967632176No Hit
GNNTANACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1150.1262224368613419No Hit
GNNTANACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1140.125124850453852No Hit
GNNTANCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1140.125124850453852No Hit
NANTNNTGGGGTNTNTNNTCNTGTNCGNTANCCNTGCNTTCGCTCCTCAG1140.125124850453852No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG1130.12402726404636205No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1110.1218320912313822No Hit
GNNTANAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1100.12073450482389227No Hit
GANTANTCGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG1090.11963691841640232No Hit
NNCTNNTCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG1090.11963691841640232No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG1090.11963691841640232No Hit
NANTNNTCGGGTNTNTNNTCNTGTNCGNTANCCNTGCNTTCGCTCCTCAG1090.11963691841640232No Hit
GANTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1070.11744174560142247No Hit
NNNTNNTCGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG1070.11744174560142247No Hit
GANTNNTCGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG1060.11634415919393254No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1050.11524657278644261No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1050.11524657278644261No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1050.11524657278644261No Hit
NANTNNCAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG1050.11524657278644261No Hit
GANTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1040.11414898637895268No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1040.11414898637895268No Hit
NANTNNTAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG1040.11414898637895268No Hit
GANTANTGGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG1030.11305139997146275No Hit
GNNTANAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1030.11305139997146275No Hit
GANTANTGGGGTNTCTNNTNNTGTTCGCTACCCATGNTTTCGCTCCTCAG1030.11305139997146275No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1020.11195381356397281No Hit
GNNTANTAGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG1020.11195381356397281No Hit
GANTANCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1010.1108562271564829No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1000.10975864074899296No Hit
NNCTNNCGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG990.10866105434150304No Hit
NANTANTCGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG990.10866105434150304No Hit
GNNTANCGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG990.10866105434150304No Hit
NANTANTGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG980.1075634679340131No Hit
NANTANTGGGGTATNTANTNNTGTTCGNTANCCNNGNTTTCGCTCCTCAG980.1075634679340131No Hit
GNNTANCCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG970.10646588152652318No Hit
NNNTNNCGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG970.10646588152652318No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG970.10646588152652318No Hit
NNCTNNTGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG960.10536829511903324No Hit
NANTNNCGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG960.10536829511903324No Hit
NNNTNNTCGGGTATNTNNTNNTGTTCGNTANCCNTGNTTTCGCTCCTCAG940.10317312230405339No Hit
GANTANCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG940.10317312230405339No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG930.10207553589656344No Hit
GANTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG930.10207553589656344No Hit
NNNTNNCGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG920.10097794948907353No Hit
NNNTNNTGGGGTATNTNNTNNTGTTCGNTANCCNTGNTTTCGCTCCTCAG920.10097794948907353No Hit
GNNTANCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG920.10097794948907353No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG920.10097794948907353No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)