FastQCFastQC Report
Wed 2 Aug 2023
EGAF00008120132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75012
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG8481.1304857889404363No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7721.0291686663467179No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG6290.8385325014664321No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5980.797205780408468No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5400.7198848184290513No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4880.650562576654402No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4740.6318988961766118No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4740.6318988961766118No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4520.6025702554257985No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4330.5772409747773689No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3970.5292486535487655No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3830.5105849730709753No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3680.4905881725590572No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3620.48258945235428996No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3500.46659201194475547No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3440.45859329173998825No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3220.4292646509891751No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3140.4185996907161521No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3130.4172665706820242No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3050.4066016104090012No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2840.3786060896923159No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2760.3679411294192929No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2570.3426118487708633No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2530.3372793686343518No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2520.33594624860022393No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2480.33061376846371243No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2460.3279475283954567No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2380.3172825681224338No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2300.3066176078494108No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2180.2906201674398763No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2170.28928704740574845No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2170.28928704740574845No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2150.2866208073374927No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2040.2719564869620861No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1930.25729216658667947No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1890.251959686450168No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1840.2452940862795286No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1800.23996160614301712No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1750.23329600597237773No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1730.230629765904122No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1720.22929664586999413No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1680.22396416573348266No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1630.21729856556284327No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1620.21596544552871538No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1620.21596544552871538No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1540.20530048525569242No Hit
GNNTANTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1530.20396736522156453No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1520.2026342451874367No Hit
GNNTANCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1480.1973017650509252No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1460.19463552498266945No Hit
GNNTANCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1390.18530368474377432No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1370.18263744467551857No Hit
GNNTANAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1330.1773049645390071No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1310.17463872447075135No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1250.1666400042659841No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1240.16530688423185622No Hit
GANTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1220.1626406441636005No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1220.1626406441636005No Hit
NANTNNTGGGGTNTNTNNTCNTGTNCGNTANCCNTGCNTTCGCTCCTCAG1210.16130752412947263No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1200.15997440409534475No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1190.1586412840612169No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1180.157308164027089No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1170.15597504399296114No Hit
GNNTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1100.14664320375406603No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1040.13864448354929879No Hit
NNNTNNTCGGGTATNTNNTNNTGTTCGNTANCCNTGNTTTCGCTCCTCAG1040.13864448354929879No Hit
NNNTNNTGGGGTATNTNNTNNTGTTCGNTANCCNTGNTTTCGCTCCTCAG1040.13864448354929879No Hit
GNNTANCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1040.13864448354929879No Hit
GNNTANTCGGGTNTNTNNTNNTGTTCGNTANCNNTGNTTTCGCTCCTNNG1020.13597824348104304No Hit
GNNTANACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1010.13464512344691515No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1000.1333120034127873No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG990.13197888337865943No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG990.13197888337865943No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG990.13197888337865943No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG980.13064576334453154No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC980.13064576334453154No Hit
GNNTANCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG980.13064576334453154No Hit
GANTNNTGGGGTATNTAATNNTGTTCGNTANCCNNGCTTTCGCTCCNCAG970.12931264331040368No Hit
NANTANTCGGGTATNTANTNNTGTTCGNTANCCNNGNTTTCGCTCCTCAG960.1279795232762758No Hit
GNNTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG960.1279795232762758No Hit
NNNTNNTGGGNNNTNTNATNNTGTTCGNTANCCNTGNTTTCGCTCCNNNG950.12664640324214793No Hit
GNNTANTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG930.12398016317389218No Hit
NNCTNNTGGGGTATNTANTCNTGTTCGNTANCCNTGCTTTCGCTCCTCAG920.1226470431397643No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG920.1226470431397643No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG920.1226470431397643No Hit
NANTNNTCGGGTNTNTNNTCNTGTNCGNTANCCNTGCNTTCGCTCCTCAG900.11998080307150856No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG890.1186476830373807No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG880.11731456300325281No Hit
NANTANTGGGGTATNTANTNNTGTTCGNTANCCNNGNTTTCGCTCCTCAG880.11731456300325281No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG860.11464832293499706No Hit
GNNTANAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG850.1133152029008692No Hit
NNNTNNTCGGNNNTNTNATNNTGTTCGNTANCCNTGNTTTCGCTCCNNNG840.11198208286674133No Hit
GNNTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG840.11198208286674133No Hit
NNNTNNCGGGGTATNTNNTNNTGTTCGNTANCCNTGNTTTCGCTCCTCAG840.11198208286674133No Hit
NANTNNCGGGGTNTNTNNTCNTGTNCGNTANCCNTGCNTTCGCTCCTCAG830.11064896283261344No Hit
GANTANTGGGGTNTCTNNTNNTGTTCGCTACCCATGNTTTCGCTCCTCAG820.10931584279848558No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG810.10798272276435769No Hit
GANTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG800.10664960273022983No Hit
GANTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG800.10664960273022983No Hit
NANTNNTGGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGAGCCTCAG800.10664960273022983No Hit
GNNTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG800.10664960273022983No Hit
GANTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG800.10664960273022983No Hit
GNNTANTAGGGTNTNTNNTNNTGTTCGNTANCNNTGNTTTCGCTCCTNNG790.10531648269610196No Hit
NNCTNNTCGGGTATNTANTCNTGTTCGNTANCCNTGCTTTCGCTCCTCAG790.10531648269610196No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC790.10531648269610196No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG780.10398336266197408No Hit
GNNTANCGGGGTNTNTNNTNNTGTTCGNTANCNNTGNTTTCGCTCCTNNG770.10265024262784621No Hit
GNNTANTGGGGTNTNTNNTNNTGTTCGNTANCNNTGNTTTCGCTCCTNNG770.10265024262784621No Hit
GANTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG760.10131712259371835No Hit
NNNTNNTGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG760.10131712259371835No Hit
NANTNNCCGGGTNTNTNNTCNTGTNCGNTANCCNTGCNTTCGCTCCTCAG760.10131712259371835No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)