FastQCFastQC Report
Wed 8 May 2024
EGAF00008120134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64897
Total Bases19.5 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5500.8474968026256993No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5130.7904833813581522No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4700.7242245404255975No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3870.596329568392992No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3830.5901659552829869No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3390.522366211072931No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2870.44223924064286485No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2830.4360756275328598No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2670.41142117509283943No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2580.397553045595328No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2310.35594865710279366No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1820.2804443965052314No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1780.2742807833952263No Hit
GNNTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1710.26349446045271735No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1680.25887175062021356No Hit
GNNTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1550.23884000801269703No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1520.23421729818019324No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1500.2311354916251907No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1240.19107200641015765No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1240.19107200641015765No Hit
GNNTANTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1230.18953110313265636No Hit
GNNTANCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1210.18644929657765383No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1210.18644929657765383No Hit
GNNTANCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1200.18490839330015255No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1160.17874478019014747No Hit
GNNTANCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1150.1772038769126462No Hit
NANTANTGGGGTATNTANTNNGAAGACNTTNTTNNTNCACGCGGCGTCGC1020.15717213430512966No Hit
GNNTANAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1010.1556312310276284No Hit
NNNTNNTGGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC1000.15409032775012713No Hit
GNNTANTGGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC990.15254942447262587No Hit
NNNTNNTGGGGTATNTNNTNNGAAGACNTTNTTNCTNCACGCGGCGTCGC970.1494676179176233No Hit
NNCTNNTCGGGTATNTANTCNGAAGACNTTNTTNCTCCACGCGGCGTCGC960.14792671464012203No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG950.14638581136262077No Hit
NANTNNTGGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC950.14638581136262077No Hit
GNNTANTCGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC940.1448449080851195No Hit
NANTNNTCGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC930.14330400480761823No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG930.14330400480761823No Hit
GANTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC910.1402221982526157No Hit
NNCTNNTGGGGTATNTANTCNGAAGACNTTNTTNCTCCACGCGGCGTCGC890.13714039169761316No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG890.13714039169761316No Hit
NANTNNCGGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC850.13097677858760806No Hit
GANTNNTGGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC840.12943587531010678No Hit
NANTNNTAGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC840.12943587531010678No Hit
NNNTNNTCGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC830.12789497203260553No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG830.12789497203260553No Hit
NANTANTCGGGTATNTANTNNGAAGACNTTNTTNNTNCACGCGGCGTCGC820.12635406875510424No Hit
GANTANTCGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC820.12635406875510424No Hit
GANTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC810.12481316547760299No Hit
GANTNNTCGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC810.12481316547760299No Hit
NNNTNNTCGGGTATNTNNTNNGAAGACNTTNTTNCTNCACGCGGCGTCGC790.12173135892260044No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG780.12019045564509916No Hit
NNNTNNTAGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC770.1186495523675979No Hit
GNNTANAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC760.11710864909009662No Hit
GNNTANTAGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC760.11710864909009662No Hit
GANTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC750.11556774581259535No Hit
GNNTANCCGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC740.11402684253509407No Hit
GANTANTGGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC740.11402684253509407No Hit
NANTANCGGGGTATNTANTNNGAAGACNTTNTTNNTNCACGCGGCGTCGC730.11248593925759282No Hit
NANTANTAGGGTATNTANTNNGAAGACNTTNTTNNTNCACGCGGCGTCGC710.10940413270259026No Hit
NNNTNNTAGGGTATNTNNTNNGAAGACNTTNTTNCTNCACGCGGCGTCGC710.10940413270259026No Hit
NNNTNNCGGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC700.10786322942508898No Hit
GANTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC700.10786322942508898No Hit
NNNTNNCCGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC680.10478142287008645No Hit
GANTANCAGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC670.10324051959258518No Hit
NNCTNNCCGGGTATNTANTCNGAAGACNTTNTTNCTCCACGCGGCGTCGC660.1016996163150839No Hit
NNCTNNTAGGGTATNTANTCNGAAGACNTTNTTNCTCCACGCGGCGTCGC660.1016996163150839No Hit
GNNTANACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC660.1016996163150839No Hit
NNNTNNCGGGGTATNTNNTNNGAAGACNTTNTTNCTNCACGCGGCGTCGC660.1016996163150839No Hit
NANTNNCAGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC650.10015871303758264No Hit
GANTNNCGGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC650.10015871303758264No Hit
GANTANTAGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC650.10015871303758264No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)