FastQCFastQC Report
Tue 8 Aug 2023
EGAF00008120140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31879
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3401.0665328272530508No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2390.749709840333762No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2030.6367828350952037No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1980.6210985288120706No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1970.617961667555444No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1640.5144452460867656No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1410.4422974371843533No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1290.4046551021048339No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1260.395244518334954No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1240.3889707958217008No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1200.3764233507951943No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1000.31368612566266196No Hit
GNNTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG980.3074124031494087No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG980.3074124031494087No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG950.29800181937952885No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG870.2729069293265159No Hit
NANTNNTCGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG830.26035948430000944No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG830.26035948430000944No Hit
NNNTNNTGGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG830.26035948430000944No Hit
GNNTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG820.2572226230433828No Hit
GANTANTCGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG800.2509489005301296No Hit
GNNTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG790.24781203927350293No Hit
NNNTNNTCGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG780.2446751780168763No Hit
GNNTANTCGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG780.2446751780168763No Hit
NANTNNTGGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG780.2446751780168763No Hit
NNNTNNTGGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG770.2415383167602497No Hit
NANTNNTAGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG740.23212773299036982No Hit
GNNTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG730.22899087173374322No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG720.2258540104771166No Hit
NANTNNCGGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG710.22271714922048996No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG680.2133065654506101No Hit
GANTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG680.2133065654506101No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG680.2133065654506101No Hit
NNNTNNTAGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG670.21016970419398348No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG660.2070328429373569No Hit
GANTNNTCGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG660.2070328429373569No Hit
GNNTANTGGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG660.2070328429373569No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG650.20389598168073028No Hit
NNNTNNCGGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG650.20389598168073028No Hit
GANTNNTGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG650.20389598168073028No Hit
NNCTNNCGGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG650.20389598168073028No Hit
NANTANTCGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG650.20389598168073028No Hit
NNNTNNCCGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG620.1944853979108504No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG610.1913485366542238No Hit
NANTANTGGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG610.1913485366542238No Hit
NNCTNNTGGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG610.1913485366542238No Hit
GANTNNTAGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG610.1913485366542238No Hit
NANTANTAGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG610.1913485366542238No Hit
NANTNNCCGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG600.18821167539759714No Hit
NNCTNNTCGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG600.18821167539759714No Hit
NNNTNNTAGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG600.18821167539759714No Hit
GNNTANTAGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG600.18821167539759714No Hit
GNNTANCAGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG580.18193795288434392No Hit
GNNTANACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG580.18193795288434392No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG570.17880109162771732No Hit
GNNTANCGGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG570.17880109162771732No Hit
NNCTNNTAGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG560.1756642303710907No Hit
GNNTANCCGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG560.1756642303710907No Hit
NNNTNNTCGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG550.17252736911446406No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG550.17252736911446406No Hit
GANTANTAGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG540.16939050785783744No Hit
GNNTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG540.16939050785783744No Hit
GANTANCAGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG530.16625364660121084No Hit
GANTANTGGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG510.15997992408795758No Hit
GANTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG510.15997992408795758No Hit
GANTNNCCGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG510.15997992408795758No Hit
NNCTNNCCGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG500.15684306283133098No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG490.15370620157470435No Hit
GNNTANAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG490.15370620157470435No Hit
NANTNNCAGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG490.15370620157470435No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG480.15056934031807773No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG470.1474324790614511No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG470.1474324790614511No Hit
NNCTNNCAGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG460.1442956178048245No Hit
NANTNNAGGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG460.1442956178048245No Hit
NANTANCAGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG460.1442956178048245No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG460.1442956178048245No Hit
NNNTNNAGGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG450.14115875654819787No Hit
GANTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG450.14115875654819787No Hit
NANTANCCGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG440.13802189529157127No Hit
NNNTNNACGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG430.13488503403494462No Hit
NNNTNNCAGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG430.13488503403494462No Hit
NNNTNNAGGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG430.13488503403494462No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG430.13488503403494462No Hit
GNNTANACGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG430.13488503403494462No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG420.13174817277831802No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG420.13174817277831802No Hit
GNNTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG410.1286113115216914No Hit
GANTANCCGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG410.1286113115216914No Hit
GANTNNAGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG410.1286113115216914No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG400.1254744502650648No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG400.1254744502650648No Hit
GANTNNCGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG390.12233758900843815No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG390.12233758900843815No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG390.12233758900843815No Hit
GANTANAGGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG380.11920072775181154No Hit
GANTNNCAGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG380.11920072775181154No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG380.11920072775181154No Hit
NNCTNNAGGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG380.11920072775181154No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG370.11606386649518491No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG370.11606386649518491No Hit
NNNTNNCAGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG370.11606386649518491No Hit
NANTNNAAGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG360.1129270052385583No Hit
GANTANCGGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG360.1129270052385583No Hit
GANTNNTGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGAGCCNCAG360.1129270052385583No Hit
GANTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG350.10979014398193167No Hit
NNNTNNTCGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG350.10979014398193167No Hit
NNNTNNCGGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG350.10979014398193167No Hit
NANTNNACGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG350.10979014398193167No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG340.10665328272530505No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG340.10665328272530505No Hit
GNNTANAGGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG340.10665328272530505No Hit
NNNTNNAAGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG330.10351642146867845No Hit
NANTANCGGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG330.10351642146867845No Hit
NNNTNNCCGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG330.10351642146867845No Hit
NNCTNNACGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG330.10351642146867845No Hit
GANTANACGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG330.10351642146867845No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG320.10037956021205181No Hit
GNNTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG320.10037956021205181No Hit
NNCTNNAAGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG320.10037956021205181No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG320.10037956021205181No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)