FastQCFastQC Report
Wed 8 May 2024
EGAF00008120142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87808
Total Bases26.4 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG10971.2493166909620992No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG9801.1160714285714286No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG8120.9247448979591837No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG7630.8689413265306123No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6610.7527787900874635No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6410.7300018221574344No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6290.7163356413994169No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6170.7026694606413995No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6060.6901421282798833No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4910.5591745626822157No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4900.5580357142857143No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4690.5341198979591837No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4670.5318422011661808No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4070.4635112973760933No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3800.432762390670554No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3490.3974580903790087No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3480.3963192419825073No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3310.37695881924198255No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3260.3712645772594752No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2910.3314048833819242No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2830.3222940962099125No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2830.3222940962099125No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2830.3222940962099125No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2710.30862791545189505No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2490.28357325072886297No Hit
GNNTANCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2400.27332361516034986No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2300.2619351311953353No Hit
GNNTANTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2290.2607962827988338No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2280.25965743440233235No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1940.2209365889212828No Hit
GACTACTGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1900.216381195335277No Hit
GACTACTCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1900.216381195335277No Hit
GNNTANCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1890.21524234693877548No Hit
GNNTANAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1820.2072704081632653No Hit
NANTNNTGGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTTCTCAG1770.20157616618075802No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1760.20043731778425655No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1730.1970207725947522No Hit
GANTANTGGGGTNTCTNNTNNTGTTCGCTCCCCATGNTTTCGCTTCTCAG1700.19360422740524783No Hit
GNNTANCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1690.19246537900874636No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1680.1913265306122449No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1660.18904883381924198No Hit
NNNTNNTGGGGTATNTNNTNNTGTTCGNTCNCCNTGNTTTCGCTTCTCAG1610.1833545918367347No Hit
GNNTANACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1580.17993804664723032No Hit
NANTNNTCGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTTCTCAG1550.17652150145772594No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1540.1753826530612245No Hit
GANTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1530.17424380466472303No Hit
NANTANTCGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTTCTCAG1520.1731049562682216No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1490.1696884110787172No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1490.1696884110787172No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1490.1696884110787172No Hit
NNNTNNTGGGNNNTNTNATNNTGTTCGNTCNCCNTGNTTTCGCTTCNNNG1470.16741071428571427No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1470.16741071428571427No Hit
NNCTNNTCGGGTATNTANTCNTGTTCGNTCNCCNTGCTTTCGCTTCTCAG1460.16627186588921283No Hit
GANTNNTCGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTTCNCAG1430.16285532069970846No Hit
NANTNNCGGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTTCTCAG1430.16285532069970846No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1410.16057762390670555No Hit
GANTANTCGGGTNTCTNNTNNTGTTCGCTCCCCATGNTTTCGCTTCTCAG1380.15716107871720117No Hit
GNNTANTCGGGTNTNTNNTNNTGTTCGNTCNCNNTGNTTTCGCTTCTNNG1380.15716107871720117No Hit
GNNTANTGGGGTNTNTNNTNNTGTTCGNTCNCNNTGNTTTCGCTTCTNNG1360.15488338192419826No Hit
NNNTNNTCGGNNNTNTNATNNTGTTCGNTCNCCNTGNTTTCGCTTCNNNG1350.1537445335276968No Hit
NNCTNNTGGGGTATNTANTCNTGTTCGNTCNCCNTGCTTTCGCTTCTCAG1350.1537445335276968No Hit
GANTNNTGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTTCNCAG1320.15032798833819241No Hit
GANTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1310.14918913994169097No Hit
GACTACTAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1300.1480502915451895No Hit
GNNTANAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1290.14691144314868804No Hit
NANTANTGGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTTCTCAG1290.14691144314868804No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1290.14691144314868804No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1280.1457725947521866No Hit
GANTANCGGGGTNTCTNNTNNTGTTCGCTCCCCATGNTTTCGCTTCTCAG1270.14463374635568513No Hit
GNNTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1250.14235604956268222No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1230.1400783527696793No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1210.1378006559766764No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1200.13666180758017493No Hit
NANTNNTAGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTTCTCAG1200.13666180758017493No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1200.13666180758017493No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1190.13552295918367346No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1170.13324526239067055No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1170.13324526239067055No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1160.1321064139941691No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1130.12868986880466474No Hit
GNNTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1130.12868986880466474No Hit
NNNTNNTAGGGTATNTNNTNNTGTTCGNTCNCCNTGNTTTCGCTTCTCAG1120.12755102040816327No Hit
GNNTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1120.12755102040816327No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1100.12527332361516036No Hit
GACTACCCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1100.12527332361516036No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1100.12527332361516036No Hit
NNNTNNTAGGNNNTNTNATNNTGTTCGNTCNCCNTGNTTTCGCTTCNNNG1090.12413447521865889No Hit
NNNTNNCGGGNNNTNTNATNNTGTTCGNTCNCCNTGNTTTCGCTTCNNNG1090.12413447521865889No Hit
NNNTNNCGGGGTATNTNNTNNTGTTCGNTCNCCNTGNTTTCGCTTCTCAG1090.12413447521865889No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG1060.12071793002915451No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1060.12071793002915451No Hit
GACTACCGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1060.12071793002915451No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1050.11957908163265306No Hit
NNNTNNTCGGGTATNTNNTNNTGTTCGNTCNCCNTGNTTTCGCTTCTCAG1040.1184402332361516No Hit
GANTNNTAGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTTCNCAG1020.11616253644314868No Hit
NNCTNNTCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG1010.11502368804664723No Hit
GANTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1010.11502368804664723No Hit
GNNTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1010.11502368804664723No Hit
NANTNNCCGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTTCTCAG1010.11502368804664723No Hit
NNCTNNCGGGGTATNTANTCNTGTTCGNTCNCCNTGCTTTCGCTTCTCAG1010.11502368804664723No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG990.11274599125364433No Hit
NANTNNAGGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTTCTCAG980.11160714285714285No Hit
GACTACAGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG970.1104682944606414No Hit
NNNTNNTGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG970.1104682944606414No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG970.1104682944606414No Hit
NANTNNCAGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTTCTCAG970.1104682944606414No Hit
GNNTANCGGGGTNTNTNNTNNTGTTCGNTCNCNNTGNTTTCGCTTCTNNG970.1104682944606414No Hit
NNCTNNTAGGGTATNTANTCNTGTTCGNTCNCCNTGCTTTCGCTTCTCAG970.1104682944606414No Hit
NANTANTAGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTTCTCAG960.10932944606413994No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA960.10932944606413994No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG960.10932944606413994No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG960.10932944606413994No Hit
GANTNNCGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTTCNCAG960.10932944606413994No Hit
GACTACACGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG950.1081905976676385No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC950.1081905976676385No Hit
GNNTANCCGGGTNTNTNNTNNTGTTCGNTCNCNNTGNTTTCGCTTCTNNG950.1081905976676385No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG950.1081905976676385No Hit
GANTNNCCGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTTCNCAG940.10705174927113702No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG940.10705174927113702No Hit
NNCTNNTGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG930.10591290087463556No Hit
GANTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG930.10591290087463556No Hit
GANTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG930.10591290087463556No Hit
NNNTNNCCGGNNNTNTNATNNTGTTCGNTCNCCNTGNTTTCGCTTCNNNG930.10591290087463556No Hit
GNNTANTGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG920.10477405247813411No Hit
GANTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG920.10477405247813411No Hit
GANTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG920.10477405247813411No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA920.10477405247813411No Hit
NNNTNNTCGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG910.10363520408163265No Hit
GANTANAGGGGTNTCTNNTNNTGTTCGCTCCCCATGNTTTCGCTTCTCAG900.10249635568513119No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG890.10135750728862973No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG890.10135750728862973No Hit
GANTANTAGGGTNTCTNNTNNTGTTCGCTCCCCATGNTTTCGCTTCTCAG890.10135750728862973No Hit
GACTACCAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG880.10021865889212828No Hit
GNNTANTAGGGTNTNTNNTNNTGTTCGNTCNCNNTGNTTTCGCTTCTNNG880.10021865889212828No Hit
NNCTNNCAGGGTATNTANTCNTGTTCGNTCNCCNTGCTTTCGCTTCTCAG880.10021865889212828No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)