FastQCFastQC Report
Wed 8 May 2024
EGAF00008120145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119225
Total Bases35.8 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC54

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC40693.41287481652338No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT36203.036275948836234No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC31482.6403858251205703No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT30032.518767037114699No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC28322.375340742293982No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC27032.267141958481862No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT26142.1924931851541203No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT24862.08513315160411No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC21591.8108618158943175No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT18901.5852379953868738No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT18561.556720486475152No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC17131.4367791989934997No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC15311.2841266512895786No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT15131.2690291465716084No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT14561.2212203816313694No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC13731.1516041098762844No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT13321.1172153491297967No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC12961.0870203396938563No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT12761.070245334451667No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT12221.0249528202977563No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC11060.9276577898930592No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC10960.9192702872719647No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC10330.8664290207590689No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT10220.8572027678758649No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC10140.8504927657789892No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT10070.844621513944223No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC8860.7431327322289788No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC8740.7330677290836654No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC7800.6542252044453764No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT7640.6408052002516251No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC7550.63325644789264No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT7230.6064164395051374No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT6590.5527364227301321No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC6430.5393164185363808No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC6310.5292514153910673No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC6290.5275739148668483No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT6180.5183476619836443No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT6170.5175089117215349No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC5760.48312015097504724No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5360.4495701404906689No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5280.44286013839379323No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT5080.42608513315160407No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5010.4202138813168379No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT4850.40679387712308657No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4730.3967288739777731No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC4710.3950513734535542No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4660.3908576221430069No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4480.3757601174250367No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT4310.36150136296917595No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC4300.36066261270706645No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4280.35898511218284757No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT4170.3497588592996435No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC3940.330467603271126No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3830.321241350387922No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3790.3178863493394842No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3740.3136925980289369No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT3590.301111344097295No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3560.2985950933109667No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT3360.2818200880687775No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3330.2793038372824492No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3230.27091633466135456No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC3230.27091633466135456No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT3210.2692388341371357No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3210.2692388341371357No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3070.2574963304676033No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3050.25581882994338434No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2870.24072132522541412No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC2700.2264625707695534No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC2510.21052631578947367No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC2510.21052631578947367No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT2470.20717131474103584No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2440.20465506395470748No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2420.20297756343048856No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT2410.20213881316837912No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT2410.20213881316837912No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2340.19626756133361295No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2330.19542881107150345No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT2230.1870413084504089No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2190.18368630740197106No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2140.17949255609142378No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT2140.17949255609142378No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2140.17949255609142378No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1990.16691130215978192No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1980.16607255189767248No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1980.16607255189767248No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1930.16187880058712517No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT1860.15600754875235898No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1840.15433004822814006No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1810.1518137974418117No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1780.14929754665548334No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1760.14762004613126442No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1760.14762004613126442No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1670.1400712937722793No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1620.13587754246173203No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1620.13587754246173203No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1610.13503879219962256No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1590.13336129167540364No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1510.126651289578528No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1410.11826378695743342No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1400.11742503669532396No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1380.11574753617110504No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1350.1132312853847767No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1340.11239253512266723No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1340.11239253512266723No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1320.11071503459844831No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1320.11071503459844831No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1310.10987628433633885No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1300.10903753407422939No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1290.10819878381211993No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC1240.10400503250157266No Hit
CCTATGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1230.1031662822394632No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)