FastQCFastQC Report
Wed 2 Aug 2023
EGAF00008120152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77875
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG8401.0786516853932584No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG8271.0619582664526486No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG7100.9117174959871589No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6300.8089887640449439No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5430.6972712680577849No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5380.6908507223113964No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5180.6651685393258426No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4870.6253611556982344No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4700.6035313001605137No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4390.5637239165329053No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4150.5329052969502408No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4140.531621187800963No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3810.4892455858747993No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3520.4520064205457464No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3400.4365971107544141No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3330.42760834670947034No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3320.4263242375601926No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3070.3942215088282504No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2930.37624398073836274No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2930.37624398073836274No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2930.37624398073836274No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2840.36468699839486357No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2830.3634028892455859No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2640.33900481540930977No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2460.3158908507223114No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2370.3043338683788122No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2330.29919743178170144No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2210.2837881219903692No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2160.27736757624398073No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2150.27608346709470305No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2150.27608346709470305No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2140.27479935794542537No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2070.2658105939004815No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2040.26195826645264847No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2010.25810593900481543No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1980.25425361155698234No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1930.24783306581059392No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1880.24141252006420547No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1820.23370786516853934No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1780.2285714285714286No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1770.2272873194221509No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1760.2260032102728732No Hit
GNNTANCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1730.22215088282504014No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1670.21444622792937398No Hit
GNNTANTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1630.20930979133226324No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1620.20802568218298553No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1560.2003210272873194No Hit
GNNTANCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1540.19775280898876405No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1530.19646869983948634No Hit
GNNTANCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1470.18876404494382024No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1450.18619582664526485No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1430.18362760834670946No Hit
GNNTANAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1370.17592295345104336No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1370.17592295345104336No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1360.17463884430176566No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1340.17207062600321027No Hit
GNNTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1300.16693418940609953No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1300.16693418940609953No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1290.16565008025682182No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1280.16436597110754414No Hit
NANTNNTGGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTTCTCAG1280.16436597110754414No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1250.16051364365971107No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1210.15537720706260033No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1170.15024077046548956No Hit
NANTNNTCGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTTCTCAG1160.14895666131621188No Hit
NNNTNNTCGGNNNTNTNATNNTGTTCGNTCNCCNTGNTTTCGCTTCNNNG1110.14253611556982343No Hit
GANTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1110.14253611556982343No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1080.13868378812199036No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1070.13739967897271269No Hit
NNNTNNTGGGNNNTNTNATNNTGTTCGNTCNCCNTGNTTTCGCTTCNNNG1060.13611556982343498No Hit
GNNTANAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1060.13611556982343498No Hit
GNNTANACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1060.13611556982343498No Hit
NNCTNNTGGGGTATNTANTCNTGTTCGNTCNCCNTGCTTTCGCTTCTCAG1050.1348314606741573No Hit
NNNTNNCGGGNNNTNTNATNNTGTTCGNTCNCCNTGNTTTCGCTTCNNNG1020.13097913322632423No Hit
GANTNNTCGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTTCNCAG1000.12841091492776885No Hit
GNNTANTCGGGTNTNTNNTNNTGTTCGNTCNCNNTGNTTTCGCTTCTNNG1000.12841091492776885No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG950.12199036918138043No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG910.11685393258426967No Hit
NANTANTGGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTTCTCAG910.11685393258426967No Hit
GNNTANTGGGGTNTNTNNTNNTGTTCGNTCNCNNTGNTTTCGCTTCTNNG910.11685393258426967No Hit
GANTNNTGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTTCNCAG910.11685393258426967No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG910.11685393258426967No Hit
GNNTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG900.11556982343499198No Hit
GANTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG870.11171749598715892No Hit
GANTANTCGGGTNTCTNNTNNTGTTCGCTCCCCATGNTTTCGCTTCTCAG870.11171749598715892No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG870.11171749598715892No Hit
GANTANTGGGGTNTCTNNTNNTGTTCGCTCCCCATGNTTTCGCTTCTCAG870.11171749598715892No Hit
GNNTANTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG850.10914927768860352No Hit
NANTNNTAGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTTCTCAG840.10786516853932583No Hit
NANTANTCGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTTCTCAG840.10786516853932583No Hit
NNNTNNCGGGGTATNTNNTNNTGTTCGNTCNCCNTGNTTTCGCTTCTCAG830.10658105939004815No Hit
GANTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG820.10529695024077046No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG820.10529695024077046No Hit
NANTNNCGGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTTCTCAG820.10529695024077046No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG810.10401284109149277No Hit
NNCTNNTCGGGTATNTANTCNTGTTCGNTCNCCNTGCTTTCGCTTCTCAG810.10401284109149277No Hit
GANTANCGGGGTNTCTNNTNNTGTTCGCTCCCCATGNTTTCGCTTCTCAG810.10401284109149277No Hit
GNNTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC800.10272873194221509No Hit
NNNTNNTGGGGTATNTNNTNNTGTTCGNTCNCCNTGNTTTCGCTTCTCAG800.10272873194221509No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG800.10272873194221509No Hit
GNNTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC790.1014446227929374No Hit
GNNTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG790.1014446227929374No Hit
GNNTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG790.1014446227929374No Hit
NNNTNNTCGGGTATNTNNTNNTGTTCGNTCNCCNTGNTTTCGCTTCTCAG780.1001605136436597No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)