FastQCFastQC Report
Wed 2 Aug 2023
EGAF00008120154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69764
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG12801.8347571813542802No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG12231.7530531506220974No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10061.4420044722206296No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9581.373201077919844No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8081.1581904707298893No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7911.133822601915028No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7631.0936872885729028No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6490.9302792271085374No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5520.7912390344590334No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3380.4844905682013646No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3370.4830571641534316No Hit
GNNTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3180.455822487242704No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2810.4027865374691818No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2710.3884524969898515No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2520.3612178200791239No Hit
GNNTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2350.33684995126426237No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG2160.3096152743535348No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2140.3067484662576687No Hit
GNNTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2100.3010148500659366No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG1960.2809471933948741No Hit
NNCTNNTGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG1940.2780803852990081No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1940.2780803852990081No Hit
GNNTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1940.2780803852990081No Hit
NNNTNNTGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG1940.2780803852990081No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1920.27521357720314205No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1890.27091336505934294No Hit
GNNTANTGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG1810.2594461326758787No Hit
NNNTNNTCGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG1800.25801272862794566No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1800.25801272862794566No Hit
NANTNNCGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG1760.2522791124362135No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1760.2522791124362135No Hit
GANTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1740.24941230434034745No Hit
GNNTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1740.24941230434034745No Hit
NANTNNCCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG1730.24797890029241446No Hit
NNNTNNTGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG1730.24797890029241446No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1730.24797890029241446No Hit
GANTANTGGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG1710.24511209219654836No Hit
NNNTNNTCGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG1700.24367868814861535No Hit
GANTANTCGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG1690.2422452841006823No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1670.23937847600481624No Hit
GNNTANTCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG1650.23651166790895017No Hit
NANTANTGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG1640.23507826386101713No Hit
NNCTNNCGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG1620.23221145576515106No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1620.23221145576515106No Hit
GANTNNTCGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG1610.23077805171721807No Hit
GANTNNTGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG1580.22647783957341897No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1570.22504443552548592No Hit
GNNTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1570.22504443552548592No Hit
GANTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1570.22504443552548592No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1530.2193108193337538No Hit
NNCTNNTCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG1520.21787741528582075No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1520.21787741528582075No Hit
NANTNNTAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG1510.2164440112378877No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1510.2164440112378877No Hit
GANTANCGGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG1500.2150106071899547No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1470.21071039504615563No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1460.20927699099822258No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1400.20067656671062437No Hit
NANTANCGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG1400.20067656671062437No Hit
NANTANTCGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG1370.1963763545668253No Hit
GANTNNCGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG1350.19350954647095925No Hit
GANTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1350.19350954647095925No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1340.1920761424230262No Hit
GANTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1330.19064273837509316No Hit
NNCTNNTAGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG1330.19064273837509316No Hit
NNNTNNTAGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG1290.18490912218336106No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1290.18490912218336106No Hit
NNNTNNCGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG1280.183475718135428No Hit
NNNTNNCGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG1280.183475718135428No Hit
GNNTANCGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG1280.183475718135428No Hit
GANTANTAGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG1250.17917550599162893No Hit
GNNTANCCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG1250.17917550599162893No Hit
NNNTNNTAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG1240.17774210194369588No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1240.17774210194369588No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1220.17487529384782982No Hit
GANTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1190.17057508170403074No Hit
GANTNNCCGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG1170.16770827360816468No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1160.16627486956023163No Hit
NNNTNNCCGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG1160.16627486956023163No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1140.1634080614643656No Hit
NNCTNNCCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG1140.1634080614643656No Hit
NNNTNNAGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG1130.16197465741643255No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1120.1605412533684995No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1120.1605412533684995No Hit
GANTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1120.1605412533684995No Hit
NANTANCCGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG1110.15910784932056649No Hit
GNNTANTAGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG1110.15910784932056649No Hit
NANTNNACGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG1110.15910784932056649No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1100.15767444527263344No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1100.15767444527263344No Hit
NANTNNCAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG1090.1562410412247004No Hit
NNNTNNCAGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG1090.1562410412247004No Hit
GANTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1090.1562410412247004No Hit
NNNTNNAGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG1070.15337423312883436No Hit
NANTNNAGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG1070.15337423312883436No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1070.15337423312883436No Hit
GNNTANCAGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG1060.1519408290809013No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1060.1519408290809013No Hit
NNCTNNAGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG1060.1519408290809013No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1050.1505074250329683No Hit
NANTANTAGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG1050.1505074250329683No Hit
NANTANCAGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG1050.1505074250329683No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1040.14907402098503525No Hit
GANTANCCGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG1030.14764061693710223No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1000.14334040479330312No Hit
NNNTNNCCGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG990.1419070007453701No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA990.1419070007453701No Hit
GANTANCAGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG980.14047359669743706No Hit
NNNTNNCAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG980.14047359669743706No Hit
GNNTANACGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG980.14047359669743706No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG980.14047359669743706No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC960.13760678860157102No Hit
GANTNNTAGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG960.13760678860157102No Hit
GANTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG950.13617338455363798No Hit
GANTNNACGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG940.13473998050570493No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG940.13473998050570493No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA930.1333065764577719No Hit
GANTANAGGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG920.1318731724098389No Hit
GNNTANAGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG910.13043976836190585No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG910.13043976836190585No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG900.12900636431397283No Hit
NNNTNNACGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG890.1275729602660398No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA890.1275729602660398No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA890.1275729602660398No Hit
NNCTNNCAGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG890.1275729602660398No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG880.12613955621810674No Hit
GANTNNCAGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG870.12470615217017372No Hit
NNNTNNACGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG870.12470615217017372No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG860.1232727481222407No Hit
NNNTNNAAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG840.12040594002637464No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG830.1189725359784416No Hit
GANTANACGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG820.11753913193050856No Hit
GANTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG810.11610572788257553No Hit
NANTANAGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG780.11180551573877645No Hit
NANTANACGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG770.1103721116908434No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG770.1103721116908434No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG760.10893870764291037No Hit
GANTNNAGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG760.10893870764291037No Hit
GNNTANAAGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG740.10607189954704432No Hit
GANTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG730.10463849549911129No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG730.10463849549911129No Hit
NNCTNNACGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG730.10463849549911129No Hit
GANTANAAGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG730.10463849549911129No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA720.10320509145117826No Hit
NNCTNNAAGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG720.10320509145117826No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC720.10320509145117826No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG710.10177168740324521No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG700.10033828335531218No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG700.10033828335531218No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA700.10033828335531218No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)