FastQCFastQC Report
Wed 2 Aug 2023
EGAF00008120156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58052
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3000.5167780610487149No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2790.4806035967753049No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2590.4461517260387239No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2310.39791910700751054No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2210.38069317163922No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2200.378970578102391No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2110.36346723627092953No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2050.35313167504995524No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1880.32384758492386134No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1860.32040239785020325No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1820.3135120237028871No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1600.27561496589264795No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1540.26527940467167366No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1540.26527940467167366No Hit
GNNTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1430.24633087576655413No Hit
GNNTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1400.241163095156067No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1380.2377179080824089No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1340.2308275339350927No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1320.22738234686143455No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1200.206711224419486No Hit
GNNTANCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1170.20154344380899883No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1110.1912078825880245No Hit
GNNTANTCGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC1100.1894852890511955No Hit
GNNTANCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1080.1860401019775374No Hit
GNNTANTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1070.18431750844070835No Hit
GANTANTGGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC1040.17914972783022118No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1010.17398194721973403No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG970.16709157307241781No Hit
NNNTNNTCGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC920.15847860538827258No Hit
GNNTANTGGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC910.15675601185144356No Hit
NNNTNNTGGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC880.15158823124095638No Hit
GNNTANCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC870.14986563770412734No Hit
GANTANTCGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC870.14986563770412734No Hit
NANTNNTCGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC860.1481430441672983No Hit
NANTANTCGGGTATNTANTNNGAAGACNTTNTTNNTNCACGCGGCGTCGC850.14642045063046924No Hit
NNCTNNTCGGGTATNTANTCNGAAGACNTTNTTNCTCCACGCGGCGTCGC840.1446978570936402No Hit
GNNTANAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC810.13953007648315302No Hit
NANTANTGGGGTATNTANTNNGAAGACNTTNTTNNTNCACGCGGCGTCGC780.13436229587266588No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG780.13436229587266588No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG780.13436229587266588No Hit
NANTNNTGGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC780.13436229587266588No Hit
NNNTNNTGGGGTATNTNNTNNGAAGACNTTNTTNCTNCACGCGGCGTCGC780.13436229587266588No Hit
GNNTANTAGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC760.13091710879900778No Hit
GNNTANAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC750.12919451526217873No Hit
GANTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC730.12574932818852064No Hit
GANTNNTCGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC730.12574932818852064No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG730.12574932818852064No Hit
NNCTNNTAGGGTATNTANTCNGAAGACNTTNTTNCTCCACGCGGCGTCGC720.12402673465169159No Hit
GANTANTAGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC720.12402673465169159No Hit
NNCTNNTGGGGTATNTANTCNGAAGACNTTNTTNCTCCACGCGGCGTCGC710.12230414111486254No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG710.12230414111486254No Hit
NNNTNNTAGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC710.12230414111486254No Hit
GNNTANACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC690.11885895404120445No Hit
NNNTNNCAGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC690.11885895404120445No Hit
GANTANCAGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC690.11885895404120445No Hit
NNNTNNTCGGGTATNTNNTNNGAAGACNTTNTTNCTNCACGCGGCGTCGC680.1171363605043754No Hit
NANTNNCCGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC680.1171363605043754No Hit
NNNTNNTAGGGTATNTNNTNNGAAGACNTTNTTNCTNCACGCGGCGTCGC680.1171363605043754No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG670.11541376696754635No Hit
GANTNNCAGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC660.11369117343071727No Hit
NNNTNNCCGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC660.11369117343071727No Hit
GANTNNTAGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC650.11196857989388823No Hit
NNNTNNCCGGGTATNTNNTNNGAAGACNTTNTTNCTNCACGCGGCGTCGC620.1068007992834011No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG620.1068007992834011No Hit
GNNTANCAGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC620.1068007992834011No Hit
GNNTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG620.1068007992834011No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG620.1068007992834011No Hit
GANTNNCGGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC620.1068007992834011No Hit
NANTANTAGGGTATNTANTNNGAAGACNTTNTTNNTNCACGCGGCGTCGC620.1068007992834011No Hit
NANTNNCAGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC610.10507820574657205No Hit
GNNTANCCGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC610.10507820574657205No Hit
NANTANCCGGGTATNTANTNNGAAGACNTTNTTNNTNCACGCGGCGTCGC600.103355612209743No Hit
GANTNNCCGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC600.103355612209743No Hit
NNCTNNCCGGGTATNTANTCNGAAGACNTTNTTNCTCCACGCGGCGTCGC590.10163301867291395No Hit
GANTNNTGGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC590.10163301867291395No Hit
GANTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC590.10163301867291395No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)