FastQCFastQC Report
Wed 8 May 2024
EGAF00008120160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87884
Total Bases26.4 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG9821.1173820035501343No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG8410.9569432433662555No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG7510.8545355240999499No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7170.8158481634882345No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG6830.7771608028765191No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6580.7487142141914341No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6140.6986482181056848No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5910.6724773565154066No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5350.6087569978608165No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5280.6007919530289928No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4880.555277411132857No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4770.5427609121114196No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA4560.5188657776159483No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4520.5143143234263347No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4140.4710755086250057No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4130.46993764507760233No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA4120.4687997815301989No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3950.44945610122434115No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3910.4449046470347276No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3610.41076874061262575No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3470.3948386509489782No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3200.36411633516908654No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3140.35728915388466614No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3120.35501342678985937No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA3070.3493241090528424No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2860.3254289745573711No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2850.3242911110099677No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2730.31063674844112693No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2710.30836102134632015No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2650.30153384006189976No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2600.2958445223248828No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2500.28446588685084884No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2480.28219015975604206No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2450.27877656911383186No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2360.26853579718720133No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2300.26170861590278094No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2200.250329980428747No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2190.24919211688134357No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2160.2457785262391334No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2150.24464066269173002No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2050.23326202721769607No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2000.2275727094806791No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2000.2275727094806791No Hit
GNNTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1990.2264348459332757No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1940.2207455281962587No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1910.21733193755404853No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1830.20822902917482133No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1820.20709116562741794No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1790.20367757498520778No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1710.1945746666059806No Hit
GNNTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1560.17750671339492968No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1530.17409312275271951No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1520.1729552592053161No Hit
GNNTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1510.1718173956579127No Hit
GNNTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1470.16726594146829912No Hit
GNNTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1440.16385235082608893No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1410.16043876018387873No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1360.15474944244686178No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1340.15247371535205498No Hit
NANTNNTGGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGAGCCTCAG1330.1513358518046516No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1320.1501979882572482No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1300.1479222611624414No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1280.14564653406763461No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1270.1445086705202312No Hit
GNNTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1270.1445086705202312No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1260.1433708069728278No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1190.13540576214100405No Hit
GANTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1180.13426789859360067No Hit
NANTNNTCGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGAGCCTCAG1180.13426789859360067No Hit
GNNTANTGGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGAGCCTNNG1160.13199217149879386No Hit
GNNTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1140.12971644440398708No Hit
NNNTNNTGGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGAGCCNNNG1140.12971644440398708No Hit
NANTNNCGGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGAGCCTCAG1130.12857858085658366No Hit
NANTNNTAGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGAGCCTCAG1090.12402712666697009No Hit
GANTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1080.1228892631195667No Hit
NNNTNNTGGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGAGCCTCAG1080.1228892631195667No Hit
NNCTNNTCGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGAGCCTCAG1070.12175139957216331No Hit
GANTNNTGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGAGCCNCAG1070.12175139957216331No Hit
GNNTANACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1070.12175139957216331No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1060.1206135360247599No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1060.1206135360247599No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG1020.11606208183514632No Hit
GNNTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1020.11606208183514632No Hit
NNCTNNTGGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGAGCCTCAG1010.11492421828774294No Hit
NNNTNNTCGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGAGCCNNNG1000.11378635474033955No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1000.11378635474033955No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG990.11264849119293616No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG990.11264849119293616No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG980.11151062764553274No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG980.11151062764553274No Hit
NANTANTCGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGAGCCTCAG970.11037276409812935No Hit
GANTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG970.11037276409812935No Hit
NNNTNNTCGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGAGCCTCAG970.11037276409812935No Hit
GNNTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG960.10923490055072596No Hit
GANTANTGGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGAGCCTCAG960.10923490055072596No Hit
NANTNNCGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG960.10923490055072596No Hit
NNNTNNCGGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGAGCCNNNG940.10695917345591917No Hit
GANTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG940.10695917345591917No Hit
NANTANCGGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGAGCCTCAG940.10695917345591917No Hit
GANTNNTCGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGAGCCNCAG930.10582130990851578No Hit
GANTANTCGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGAGCCTCAG930.10582130990851578No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG920.10468344636111239No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA920.10468344636111239No Hit
GANTANCGGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGAGCCTCAG910.10354558281370897No Hit
GNNTANTCGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGAGCCTNNG910.10354558281370897No Hit
NNCTNNTCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG900.10240771926630558No Hit
NNCTNNTGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG900.10240771926630558No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG900.10240771926630558No Hit
NNNTNNCGGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGAGCCTCAG890.1012698557189022No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG890.1012698557189022No Hit
GANTNNCGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGAGCCNCAG890.1012698557189022No Hit
GANTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG880.10013199217149879No Hit
GANTANTGGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG880.10013199217149879No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)