FastQCFastQC Report
Wed 8 May 2024
EGAF00008120168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76839
Total Bases23.1 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9781.2727911607386873No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9761.2701883158292013No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7871.024219471882768No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7230.9409284347792137No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6260.8146904566691393No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6170.8029776545764521No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6080.7912648524837647No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5990.7795520503910774No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5690.7405093767487864No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5060.6585197620999752No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4970.6468069600072879No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4620.6012571740912818No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4480.5830372597248793No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4350.5661187678132199No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4290.5583102330847616No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3770.49063626543812383No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3670.47762204089069354No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3620.47111492861697835No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3520.45810070406954795No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3470.45159359179583286No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3180.41385234060828485No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3080.40083811606085445No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2900.3774125118754799No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2640.343575528052161No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2530.3292598810499876No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2390.31103996668358513No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2310.3006285870456409No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2110.2746001379507802No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2070.2693944481318081No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1940.2524759562201486No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1880.2446674214916904No Hit
GNNTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1850.24076315412746133No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1800.23425604185374613No Hit
GNNTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1760.22905035203477397No Hit
GNNTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1620.2108304376683715No Hit
GNNTANTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1620.2108304376683715No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1540.20041905803042723No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1480.19261052330196907No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1470.19130910084722602No Hit
GNNTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1450.18870625593773996No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG1430.1861034110282539No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1430.1861034110282539No Hit
GNNTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1430.1861034110282539No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1390.18089772120928174No Hit
NNCTNNTGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG1350.17569203139030962No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1350.17569203139030962No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG1340.17439060893556657No Hit
GANTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1320.1717877640260805No Hit
NNNTNNTCGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG1280.16658207420710838No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1260.16397922929762232No Hit
GNNTANCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1190.15486927211442106No Hit
GNNTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1170.152266427204935No Hit
NNCTNNTCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG1150.14966358229544893No Hit
NNNTNNTGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG1150.14966358229544893No Hit
GANTNNTGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG1140.1483621598407059No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1130.14706073738596287No Hit
GANTANTGGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG1120.14575931493121982No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1120.14575931493121982No Hit
NNNTNNTGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG1110.1444578924764768No Hit
GNNTANTGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG1110.1444578924764768No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1110.1444578924764768No Hit
NNNTNNTCGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG1070.13925220265750465No Hit
GNNTANTCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG1060.13795078020276164No Hit
GANTANTGGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG1050.1366493577480186No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1040.13534793529327557No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1040.13534793529327557No Hit
GANTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1040.13534793529327557No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1030.13404651283853253No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1030.13404651283853253No Hit
GANTANTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1030.13404651283853253No Hit
NANTANTGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG1020.13274509038378948No Hit
NNNTNNTAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG1020.13274509038378948No Hit
NNNTNNCGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG1000.13014224547430342No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1000.13014224547430342No Hit
NNNTNNTGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG990.1288408230195604No Hit
GANTANTCGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG980.12753940056481736No Hit
GANTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG980.12753940056481736No Hit
GNNTANCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG960.1249365556553313No Hit
NANTNNCCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG950.12363513320058823No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG950.12363513320058823No Hit
NANTANTCGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG930.12103228829110216No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG920.11973086583635913No Hit
NNNTNNCGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG910.1184294433816161No Hit
GNNTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG910.1184294433816161No Hit
GNNTANAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG910.1184294433816161No Hit
NANTNNCGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG900.11712802092687306No Hit
NNCTNNTAGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG900.11712802092687306No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG900.11712802092687306No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG900.11712802092687306No Hit
GNNTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG900.11712802092687306No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG890.11582659847213003No Hit
GANTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG890.11582659847213003No Hit
GNNTANCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG890.11582659847213003No Hit
GANTNNCGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG890.11582659847213003No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG880.11452517601738699No Hit
GANTANTAGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG880.11452517601738699No Hit
GANTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG880.11452517601738699No Hit
NNNTNNTCGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG880.11452517601738699No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG880.11452517601738699No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC880.11452517601738699No Hit
NANTANCGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG880.11452517601738699No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG870.11322375356264396No Hit
NNCTNNTGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG870.11322375356264396No Hit
NANTNNCCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG860.11192233110790092No Hit
GNNTANTAGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG860.11192233110790092No Hit
NANTANTCGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG860.11192233110790092No Hit
GNNTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG850.11062090865315789No Hit
GNNTANTGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG850.11062090865315789No Hit
NANTNNTAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG840.10931948619841486No Hit
GANTNNTGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG840.10931948619841486No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG840.10931948619841486No Hit
NNCTNNCCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG840.10931948619841486No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG840.10931948619841486No Hit
NNNTNNTGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG840.10931948619841486No Hit
GANTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG830.10801806374367183No Hit
NANTNNCAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG830.10801806374367183No Hit
NANTANCCGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG820.10671664128892878No Hit
NNNTNNCAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG820.10671664128892878No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG820.10671664128892878No Hit
GANTNNTCGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG820.10671664128892878No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA810.10541521883418575No Hit
GNNTANTCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG810.10541521883418575No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC810.10541521883418575No Hit
GANTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG800.10411379637944272No Hit
NANTNNAGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG800.10411379637944272No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG800.10411379637944272No Hit
NNCTNNCGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG790.10281237392469969No Hit
GANTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG790.10281237392469969No Hit
NANTANTGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG790.10281237392469969No Hit
GNNTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG780.10151095146995666No Hit
GNNTANACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG780.10151095146995666No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG770.10020952901521361No Hit
GANTNNCAGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG770.10020952901521361No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTTCTCAG770.10020952901521361No Hit
GANTNNCGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG770.10020952901521361No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)