FastQCFastQC Report
Wed 2 Aug 2023
EGAF00008120176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67991
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG11451.6840464179082526No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG9881.4531335029636276No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG7831.1516230089276522No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG7201.0589636863702547No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6350.9339471400626553No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6220.9148269623920813No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5940.8736450412554603No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4980.7324498830727597No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4140.6089041196628966No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3220.4735920930711418No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3100.45594269829830425No Hit
GNNTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2660.3912282507978997No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2350.3456339809680693No Hit
GNNTANTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2260.33239693488844113No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2200.3235722375020223No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2110.31033519142239413No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2060.3029812769337118No Hit
GNNTANCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2020.29709814534276596No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1860.2735656189789825No Hit
GNNTANAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1740.2559162242061449No Hit
GNNTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1720.25297465841067196No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1690.24856230971746263No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1490.2191466517627333No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1470.2162050859672604No Hit
GNNTANCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1450.21326352017178746No Hit
GNNTANCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1420.20885117147857804No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG1400.2059096056831051No Hit
GANTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1380.2029680398876322No Hit
NNCTNNTGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG1370.20149725698989573No Hit
NNNTNNTGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG1350.1985556911944228No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1280.18826021091026754No Hit
GANTANTGGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG1270.18678942801253107No Hit
GANTANTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1260.1853186451147946No Hit
GANTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1260.1853186451147946No Hit
GANTNNTGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG1220.17943551352384873No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1200.17649394772837582No Hit
NNNTNNTGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG1190.17502316483063934No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1170.17208159903516643No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG1140.167669250341957No Hit
GNNTANAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1130.16619846744422057No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1120.1647276845464841No Hit
NANTANTCGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG1120.1647276845464841No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1100.16178611875101115No Hit
NANTANTGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG1080.15884455295553823No Hit
NNNTNNCGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG1070.15737377005780176No Hit
NNNTNNTCGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG1070.15737377005780176No Hit
NNCTNNTCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG1060.1559029871600653No Hit
NANTNNCGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG1060.1559029871600653No Hit
GNNTANTCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG1040.15296142136459237No Hit
GANTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1040.15296142136459237No Hit
NANTNNCCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG1040.15296142136459237No Hit
GNNTANACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1040.15296142136459237No Hit
NANTANCGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG1040.15296142136459237No Hit
GNNTANTGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG1040.15296142136459237No Hit
NANTNNTAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG1040.15296142136459237No Hit
NNNTNNTCGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG1000.1470782897736465No Hit
NNNTNNCGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG980.14413672397817356No Hit
GANTANTCGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG980.14413672397817356No Hit
GANTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG970.14266594108043712No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG970.14266594108043712No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG960.14119515818270065No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG950.13972437528496418No Hit
NNCTNNTAGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG940.1382535923872277No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG940.1382535923872277No Hit
GANTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG930.13678280948949126No Hit
GNNTANCGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG930.13678280948949126No Hit
GANTANCGGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG910.1338412436940183No Hit
GANTNNCCGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG910.1338412436940183No Hit
GANTNNTCGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG910.1338412436940183No Hit
GANTANCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG900.13237046079628184No Hit
NNNTNNTAGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG900.13237046079628184No Hit
GANTANAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG890.1308996778985454No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG890.1308996778985454No Hit
GNNTANCCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG880.12942889500080892No Hit
NNNTNNTAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG880.12942889500080892No Hit
GANTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG880.12942889500080892No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG880.12942889500080892No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG880.12942889500080892No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG870.12795811210307245No Hit
GANTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG860.12648732920533598No Hit
NNNTNNCCGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG860.12648732920533598No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG860.12648732920533598No Hit
NNCTNNCGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG850.12501654630759954No Hit
NNCTNNCAGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG840.12354576340986308No Hit
GANTANCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG840.12354576340986308No Hit
GANTNNCGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG830.1220749805121266No Hit
NANTANCCGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG820.12060419761439013No Hit
NNNTNNCAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG810.11913341471665369No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG800.11766263181891722No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG800.11766263181891722No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG790.11619184892118074No Hit
GANTANCCGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG790.11619184892118074No Hit
NNCTNNCCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG780.11472106602344427No Hit
GNNTANTAGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG780.11472106602344427No Hit
GNNTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC770.11325028312570783No Hit
NANTANTAGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG770.11325028312570783No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG760.11177950022797135No Hit
GANTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG750.11030871733023488No Hit
NANTNNACGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG750.11030871733023488No Hit
GANTNNTAGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG750.11030871733023488No Hit
NANTNNCAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG750.11030871733023488No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG740.10883793443249841No Hit
NANTNNAGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG730.10736715153476196No Hit
GANTANTAGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG730.10736715153476196No Hit
NNCTNNACGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG730.10736715153476196No Hit
GANTANCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG730.10736715153476196No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG710.10442558573928902No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG710.10442558573928902No Hit
NNNTNNCCGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG700.10295480284155255No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG700.10295480284155255No Hit
GNNTANCAGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG690.1014840199438161No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG680.10001323704607963No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG680.10001323704607963No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)