FastQCFastQC Report
Tue 8 Aug 2023
EGAF00008120177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45989
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7461.6221270303768291No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5881.2785666137554632No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5511.1981125921415992No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4941.0741699101959163No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4590.9980647546152341No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4470.9719715584161429No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4360.9480527952336427No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4140.9002152688686424No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3810.828458979321142No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3730.8110635151884146No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3670.7980169170888691No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3650.7936680510556873No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3290.7153884624584139No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2970.6458066059275044No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2890.6284111417947771No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2770.602317945595686No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2590.5631781512970493No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2410.5240383569984126No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2250.48924742873295785No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2050.4457587684011394No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1970.428363304268412No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1890.41096784013568466No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1790.3892235099697754No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1720.3740024788536389No Hit
CCTACGGGTGGCTGCAGCGCTAGATCTCGTATGCCGTCTTCTGCTTGAAA1550.33703711757159316RNA PCR Primer, Index 11 (96% over 29bp)
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1400.3044206223227294No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1360.29572289025636567No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1340.2913740242231838No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1320.28702515819000196No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1310.28485072517341103No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1310.28485072517341103No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1200.26093196199091084No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1190.2587575289743199No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1150.25005979690795627No Hit
CCTACGGGGGGCTGCAGCGCTAGATCTCGTATGCCGTCTTCTGCTTGAAA1060.23048989975863796Illumina PCR Primer Index 11 (96% over 29bp)
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1040.22614103372545608No Hit
CCTACGGGCGGCTGCAGCGCTAGATCTCGTATGCCGTCTTCTGCTTGAAA1020.22179216769227425Illumina PCR Primer Index 11 (96% over 29bp)
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1020.22179216769227425No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT980.21309443562591057No Hit
CCTACGGGTGGCTGCAGTGAGCAGTTCTTGTCCTTCAGGGTAGATGGGCA970.21092000260931962No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT910.1978734045097741No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT910.1978734045097741No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT880.1913501054600013No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC870.18917567244341038No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT800.17395464132727392No Hit
CCTACGGGTGGCTGCAGAAACACAGGGATCCCAGTAGCAGACAGGTGGTG750.1630824762443193No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC730.15873361021113744No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC710.15438474417795559No Hit
CCTACGGGTGGCTGCAGAATGCAGGGTCCTCGTTGCATATCCAGTTTGTT710.15438474417795559No Hit
CCTACGGGGGGCTGCAGTGAGCAGTTCTTGTCCTTCAGGGTAGATGGGCA700.1522103111613647No Hit
CCTACGGGGGGCAGCAGCGCTAGATCTCGTATGCCGTCTTCTGCTTGAAA700.1522103111613647Illumina PCR Primer Index 11 (96% over 29bp)
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT690.15003587814477376No Hit
CCTACGGGTGGCTGCAGAGTTAGCATGGCATTCTTTAAGCTGATGTTGGA670.1456870121115919No Hit
CCTACGGGGGGCTGCAGAAACACAGGGATCCCAGTAGCAGACAGGTGGTG660.14351257909500098No Hit
CCTACGGGTGGCTGCAGGGAGCAGGTGAGCGATGGTGTGCACTCCAGAGG640.13916371306181913No Hit
CCTACGGGTGGCTGCAGGGTCAGATTCCTGCAGGAAACTCCCTCATGGCC640.13916371306181913No Hit
CCTACGGGTGGCAGCAGTGAGCAGTTCTTGTCCTTCAGGGTAGATGGGCA610.13264041401204635No Hit
CCTACGGGTGGCAGCAGCGCTAGATCTCGTATGCCGTCTTCTGCTTGAAA610.13264041401204635RNA PCR Primer, Index 11 (96% over 29bp)
CCTACGGGTGGCTGCAGTGATAAATATTAAGCAATGAACGAAAGTTTGAC600.13046598099545542No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT600.13046598099545542No Hit
CCTACGGGCGGCTGCAGTGAGCAGTTCTTGTCCTTCAGGGTAGATGGGCA600.13046598099545542No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT600.13046598099545542No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT600.13046598099545542No Hit
CCTACGGGTGGCTGCAGCCTATCCTGTTCCTGACTGGCATGCTCTGCACC590.12829154797886452No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT570.12394268194568267No Hit
CCTACGGGGGGCAGCAGTGAGCAGTTCTTGTCCTTCAGGGTAGATGGGCA570.12394268194568267No Hit
CCTACGGGGGGCTGCAGAGTTAGCATGGCATTCTTTAAGCTGATGTTGGA570.12394268194568267No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC560.12176824892909173No Hit
CCTACGGGTGGCTGCAGAGATGGCTCAGCGGTTAAGAACACATGTTGTTC550.11959381591250082No Hit
CCTACGGGGGGCTGCAGAATGCAGGGTCCTCGTTGCATATCCAGTTTGTT550.11959381591250082No Hit
CCTACGGGTGGCTGCAGTAGCCTATAATGTCTTGGAGGTCCCCCTGACCC540.11741938289590989No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT530.11524494987931898No Hit
CCTACGGGCGGCAGCAGCGCTAGATCTCGTATGCCGTCTTCTGCTTGAAA530.11524494987931898Illumina PCR Primer Index 11 (96% over 29bp)
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC510.11089608384613713No Hit
CCTACGGGTGGCTGCAGAATCAATTAGATACCCTGGTAGTCCGGCTGGCT510.11089608384613713No Hit
CCTACGGGTGGCAGCAGAGTTAGCATGGCATTCTTTAAGCTGATGTTGGA500.1087216508295462No Hit
CCTACGGGCGGCTGCAGAAACACAGGGATCCCAGTAGCAGACAGGTGGTG500.1087216508295462No Hit
CCTACGGGGGGCTGCAGTGATAAATATTAAGCAATGAACGAAAGTTTGAC500.1087216508295462No Hit
CCTACGGGTGGCTGCAGGGCCAGAGAACAGGAAGCTTGGGAACTCAGGTA490.10654721781295529No Hit
CCTACGGGTGGCTGCAGCTGATGCAGAGAGCATGGAGGTCTACTGCTTAC490.10654721781295529No Hit
CCTACGGGAGGCAGCAGCGCTAGATCTCGTATGCCGTCTTCTGCTTGAAA480.10437278479636435Illumina PCR Primer Index 11 (96% over 29bp)
CCTACGGGCGGCTGCAGAGTTAGCATGGCATTCTTTAAGCTGATGTTGGA470.10219835177977343No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAGGATTAGA470.10219835177977343No Hit
CCTACGGGGGGCAGCAGGGAGCAGGTGAGCGATGGTGTGCACTCCAGAGG470.10219835177977343No Hit
CCTACGGGGGGCTGCAGCTGATGCAGAGAGCATGGAGGTCTACTGCTTAC460.1000239187631825No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT460.1000239187631825No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)