FastQCFastQC Report
Tue 8 Aug 2023
EGAF00008120183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41741
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC6321.514098847655782No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5711.3679595601447019No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5111.2242159986583936No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5031.205050190460219No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4431.0613066289739106No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3900.9343331496610048No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3860.9247502455619175No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3630.8696485469921659No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3620.8672528209673942No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3590.8600656428930786No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2960.7091349033324549No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2860.6851776430847368No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2760.6612203828370187No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2620.6276802184902135No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2550.6109101363168108No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2450.5869528760690927No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2360.5653913418461465No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2330.558204163771831No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2250.5390383555736565No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2180.522268273400254No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2150.5150810953259385No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2150.5150810953259385No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2120.507893917251623No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2000.4791452049543614No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1860.44560504060755607No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1670.4000862461368918No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1590.3809204379387173No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1500.35935890371577106No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1450.34738027359191204No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1420.34019309551759663No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1310.31384010924510675No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1280.3066529311707913No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1180.28269567092307324No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1160.2779042188735296No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1160.2779042188735296No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1140.273112766823986No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1130.2707170407992142No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1130.2707170407992142No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1130.2707170407992142No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1080.25873841067535513No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1020.24436405452672433No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1000.2395726024771807No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1000.2395726024771807No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC990.23717687645240892No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC980.23478115042763711No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT940.22519824632854984No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT920.22040679427900628No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT880.210823890179919No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC870.2084281641551472No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC840.2012409860808318No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT830.19884526005605999No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT830.19884526005605999No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC810.1940538080065164No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT810.1940538080065164No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC810.1940538080065164No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT790.18926235595697274No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC770.18447090390742915No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC770.18447090390742915No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT740.17728372583311372No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC720.17249227378357013No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT690.16530509570925467No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT680.16290936968448289No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT670.16051364365971107No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT650.15572219161016748No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC640.15332646558539564No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC620.14853501353585205No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC610.14613928751108024No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC610.14613928751108024No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT600.14374356148630843No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT600.14374356148630843No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC600.14374356148630843No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT590.14134783546153662No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT590.14134783546153662No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC560.13416065738722122No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC540.12936920533767757No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCTTCACGGGAT540.12936920533767757No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT540.12936920533767757No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT540.12936920533767757No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT540.12936920533767757No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCTTCACGGGAT530.12697347931290576No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT520.12457775328813397No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCTGCAATGGGCGAAAGCCTGAC510.12218202726336216No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG510.12218202726336216No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT500.11978630123859035No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC480.11499484918904675No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG470.11259912316427492No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT460.11020339713950314No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT460.11020339713950314No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC460.11020339713950314No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT440.1054119450899595No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT440.1054119450899595No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT440.1054119450899595No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT440.1054119450899595No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC430.10301621906518771No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC420.1006204930404159No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT420.1006204930404159No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCTGCAATGGGCGAAAGCCTGAC420.1006204930404159No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)