FastQCFastQC Report
Wed 8 May 2024
EGAF00008120190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111147
Total Bases33.4 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG80907.278648996374171No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG76426.875579187922301No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG60555.447740379857306No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG60375.431545610767722No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG50534.546231567203793No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG47024.2304335699569044No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG45094.056789656940809No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG40903.679811420911046No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG34813.1318884000467846No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6610.5947079093452815No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6560.5902093623759526No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6360.5722151744986369No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6020.5416250551072004No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5070.45615266268995114No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5050.45435324390221954No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5020.45165411572062225No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4980.44805527814515916No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4930.4435567311758302No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4330.3895741675438833No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA4310.38777474875615175No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4250.38237649239295707No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4230.3805770736052255No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4170.37517881724203084No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4020.3616831763340441No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3850.3463881166383258No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA3810.3427892790628627No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3780.3400901508812654No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3730.33559160391193643No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3520.31669770664075503No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3410.3068009033082314No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3260.29330526240024474No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3150.2834084590677211No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA3140.28250874967385536No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2980.26811339937200285No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2950.2654142711904055No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA2890.2600160148272108No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2840.2555174678578819No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2740.24652037391922407No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2480.22312792967871378No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA2310.20783286998299547No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA2250.2024346136198008No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2240.201534904225935No Hit
GACTACTGGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGAGCCTCAG2200.1979360666504719No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA2170.19523693846887455No Hit
GACTACTCGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGAGCCTCAG2100.1889389727118141No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA2060.18534013513635098No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2030.18264100695475363No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1980.1781424599854247No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGNTTTCGAGCCTCAG1760.15834885332037751No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1750.15744914392651174No Hit
GACTACCGGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGAGCCTCAG1720.15475001574491437No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1710.1538503063510486No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGNTTTCGAGCCTCAG1680.15115117816945126No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGNTTTCGAGCCTCAG1620.14575292180625657No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1590.14305379362465923No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1580.14215408423079345No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGNTTTCGAGCCTCAG1570.14125437483692768No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1560.14035466544306188No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1520.13675582786759877No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1510.135856118473733No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1480.13315699029213565No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1470.13225728089826988No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1460.13135757150440408No Hit
GACTACCAGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGAGCCTCAG1460.13135757150440408No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGNTTTCGAGCCTCAG1380.12415989635347782No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1360.12236047756574626No Hit
GACTACCCGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGAGCCTCAG1360.12236047756574626No Hit
GACTACTAGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGAGCCTCAG1310.11786193059641736No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1290.11606251180868579No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1270.11426309302095423No Hit
GACTACAGGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGAGCCTCAG1250.11246367423322268No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1200.10796512726389375No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGNTTTCGAGCCTCAG1190.10706541787002798No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1190.10706541787002798No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1180.10616570847616219No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1180.10616570847616219No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1170.1052659990822964No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGNTTTCGAGCCTCAG1170.1052659990822964No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1160.10436628968843063No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGNTTTCGAGCCTCAG1150.10346658029456485No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1120.1007674521129675No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1120.1007674521129675No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)