FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008120202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97986
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG9971.017492294817627No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG8540.8715530790112873No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6940.7082644459412569No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6930.7072438919845692No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5370.5480374747412896No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5290.539873043087788No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5230.5337497193476619No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5140.5245647337374727No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5070.5174208560406589No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4600.46945482007633743No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4170.4255709999387668No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4120.42046823015532825No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4000.408221582675076No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3990.40720102871838837No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3770.38474884167125917No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3630.37046108627763147No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3490.3561733308840038No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3390.34596779131712696No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3150.32147449635662234No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2980.3041250790929316No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2950.30106341722286856No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2920.29800175535280554No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2710.276570122262364No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2650.2704467985222379No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2640.2694262445655502No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2600.2653440287387994No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2420.246974057518421No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2400.2449329496050456No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2340.23880962586491947No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2290.23370685608148103No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2290.23370685608148103No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2280.23268630212479333No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2210.2255424244279795No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2190.22350131651460414No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2150.21941910068785336No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2070.21125466903435183No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2050.20921356112097647No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2020.2061518992509134No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2010.2051313452942257No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1950.19900802155409955No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1910.1949258057273488No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1880.19186414385728573No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1850.18880248198722266No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1830.1867613740738473No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1810.1847202661604719No Hit
GNNTANCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1790.18267915824709652No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1750.17859694242034577No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1740.17757638846365806No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1720.1755352805502827No Hit
GNNTANTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1710.174514726593595No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1630.16635029494009348No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1630.16635029494009348No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1580.16124752515665502No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1510.1541036474598412No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1500.15308309350315352No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1500.15308309350315352No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1460.14900087767640277No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1440.14695976976302735No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1430.1459392158063397No Hit
GNNTANCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1400.1428775539362766No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1400.1428775539362766No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1380.14083644602290124No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1330.13573367623946278No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1320.1347131222827751No Hit
GNNTANCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1320.1347131222827751No Hit
NANTNNTGGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTTCTCAG1320.1347131222827751No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1280.13063090645602432No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1210.1234870287592105No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1200.1224664748025228No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1200.1224664748025228No Hit
NNNTNNTGGGGTATNTNNTNNTGTTCGNTCNCCNTGNTTTCGCTTCTCAG1190.12144592084583512No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1170.11940481293245973No Hit
GANTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1160.11838425897577205No Hit
GNNTANAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1130.11532259710570897No Hit
NANTNNTCGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTTCTCAG1120.11430204314902129No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1110.11328148919233359No Hit
GNNTANTGGGGTNTNTNNTNNTGTTCGNTCNCNNTGNTTTCGCTTCTNNG1100.1122609352356459No Hit
NNNTNNTCGGGTATNTNNTNNTGTTCGNTCNCCNTGNTTTCGCTTCTCAG1100.1122609352356459No Hit
GANTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1090.11124038127895823No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1090.11124038127895823No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1090.11124038127895823No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1080.11021982732227054No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1080.11021982732227054No Hit
GANTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1070.10919927336558283No Hit
GNNTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1060.10817871940889516No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1060.10817871940889516No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1060.10817871940889516No Hit
GANTANTGGGGTNTCTNNTNNTGTTCGCTCCCCATGNTTTCGCTTCTCAG1060.10817871940889516No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1040.10613761149551977No Hit
GANTNNTGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTTCNCAG1040.10613761149551977No Hit
NNCTNNTGGGGTATNTANTCNTGTTCGNTCNCCNTGCTTTCGCTTCTCAG1040.10613761149551977No Hit
GANTANTCGGGTNTCTNNTNNTGTTCGCTCCCCATGNTTTCGCTTCTCAG1030.10511705753883206No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1020.1040965035821444No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1020.1040965035821444No Hit
NANTANTCGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTTCTCAG1010.1030759496254567No Hit
NNNTNNTCGGNNNTNTNATNNTGTTCGNTCNCCNTGNTTTCGCTTCNNNG1000.102055395668769No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)