FastQCFastQC Report
Wed 2 Aug 2023
EGAF00008120208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69877
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA29594.234583625513403No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA24033.4388997810438338No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA20983.002418535426535No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA19602.8049286603603476No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA18372.6289050760622237No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA15972.2854444237732014No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA14272.0421597950684776No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA13271.8990511899480516No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA10981.5713324842222764No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG8311.1892325085507391No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG7611.089056484966441No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6500.9302059332827683No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6380.9130329006683172No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6280.8987220401562745No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5300.7584756071382572No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4720.6754726161684101No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3950.5652789902256822No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3660.5237774947407587No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTANCCACGCTTTCGAGCATGAA2970.4250325572076649No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2730.39068649197876265No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTANCCACGCTTTCGAGCATGAA2700.3863932338251499No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2390.3420295662378179No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTANCCACGCTTTCGAGCATGAA2240.320563275469754No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2110.3019591568040986No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTANCCACGCTTTCGAGCATGAA2090.2990969847016901No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTANCCACGCTTTCGAGCATGAA2080.2976658986504858No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1930.276199607882422No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTANCCACGCTTTCGAGCATGAA1790.2561644031655623No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1610.23040485424388568No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1590.2275426821414772No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1590.2275426821414772No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1580.2261115960902729No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTANCCACGCTTTCGAGCATGAA1500.21466290768063884No Hit
GACTACTCGGGTATCTAATCCTGTTTGNTANCCACGCTTTCGAGCATGAA1400.20035204716859623No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1330.19033444481016645No Hit
GACTACACGGGTATCTAATCCTGTTTGCTANCCACGCTTTCGAGCATGAA1310.18747227270775793No Hit
GACTACTGGGGTATCTAATCCTGTTTGNTACCCACGCTTTCGAGCATGAA1310.18747227270775793No Hit
GACTACTGGGGTATCTAATCCTGTTTGNTANCCACGCTTTCGAGCATGAA1310.18747227270775793No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1300.18604118665655364No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1270.1817479285029409No Hit
GACTACTCGGGTATCTAATCNTGTTTGCTACCCACGCTTTCGAGCATGAA1250.17888575640053236No Hit
GACTACTCGGGTATCTAATNCTGTTTGCTACCCACGCTTTCGAGCATGAA1230.17602358429812384No Hit
GACTACTCGGGTATCTAATCCTGTTTGNTACCCACGCTTTCGAGCATGAA1230.17602358429812384No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTANCCACGCTTTCGAGCATGAA1220.17459249824691958No Hit
GACTACTAGGGTATCTAATCNTGTTTGCTACCCACGCTTTCGAGCATGAA1150.16457489588848978No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1140.16314380983728552No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1130.16171272378608126No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1130.16171272378608126No Hit
GACTACTGGGGTATCTAATCNTGTTTGCTACCCACGCTTTCGAGCATGAA1100.15741946563246847No Hit
GACTACCGGGGTATCTAATNCTGTTTGCTACCCACGCTTTCGAGCATGAA1100.15741946563246847No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1070.15312620747885572No Hit
GACTACTGGGGTATCTAATNCTGTTTGCTACCCACGCTTTCGAGCATGAA1070.15312620747885572No Hit
GACTACTGGGGTATCTAATNNTGTTTGCTACCCACGCTTTCGAGCATGAA1050.1502640353764472No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1050.1502640353764472No Hit
GACTACTCGGGTATCTAATNNTGTTTGCTACCCACGCTTTCGAGCATGAA1040.1488329493252429No Hit
GACTACTAGGGTATCTAATCCTGTTTGNTANCCACGCTTTCGAGCATGAA1040.1488329493252429No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1020.1459707772228344No Hit
GACTACCGGGGTATCTAATCCTGTTTGNTANCCACGCTTTCGAGCATGAA980.14024643301801737No Hit
GACTACCCGGGTATCTAATNCTGTTTGCTACCCACGCTTTCGAGCATGAA950.1359531748644046No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCNCCATGCTTTCGCTTCTCAG880.12593557250597479No Hit
GACTACCGGGGTATCTAATCCTGTTTGNTACCCACGCTTTCGAGCATGAA870.12450448645477052No Hit
GACTACCCGGGTATCTAATCCTGTTTGNTANCCACGCTTTCGAGCATGAA870.12450448645477052No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC870.12450448645477052No Hit
GACTACTAGGGTATCTAATCCTGTTTGNTACCCACGCTTTCGAGCATGAA860.12307340040356626No Hit
GACTACCGGGGTATCTAATNNTGTTTGCTACCCACGCTTTCGAGCATGAA860.12307340040356626No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCNCCATGCTTTCGCTTCTCAG850.121642314352362No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG850.121642314352362No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC850.121642314352362No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG840.12021122830115774No Hit
GACTACCAGGGTATCTAATCCTGTTTGNTANCCACGCTTTCGAGCATGAA840.12021122830115774No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCNCCATGCTTTCGCTTCTCAG840.12021122830115774No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG820.11734905619874922No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG820.11734905619874922No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG810.11591797014754497No Hit
GACTACTAGGGTATCTAATNCTGTTTGCTACCCACGCTTTCGAGCATGAA800.11448688409634071No Hit
GACTACCCGGGTATCTAATNNTGTTTGCTACCCACGCTTTCGAGCATGAA790.11305579804513645No Hit
GACTACCGGGGTATCTAATCNTGTTTGCTACCCACGCTTTCGAGCATGAA790.11305579804513645No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG780.1116247119939322No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC760.10876253989152368No Hit
GACTACCAGGGTATCTAATNNTGTTTGCTACCCACGCTTTCGAGCATGAA760.10876253989152368No Hit
GACTACCAGGGTATCTAATCNTGTTTGCTACCCACGCTTTCGAGCATGAA750.10733145384031942No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG740.10590036778911516No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG740.10590036778911516No Hit
GACTACCAGGGTATCTAATCCTGTTTGNTACCCACGCTTTCGAGCATGAA730.1044692817379109No Hit
GACTACTAGGGTATCTAATNNTGTTTGCTACCCACGCTTTCGAGCATGAA720.10303819568670664No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG720.10303819568670664No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTGAA710.10160710963550239No Hit
GACTACCCGGGTATCTAATCNTGTTTGCTACCCACGCTTTCGAGCATGAA700.10017602358429811No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)