FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008120239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119679
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT58484.886404465277952No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT46683.9004336600406084No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT42253.5302768238370974No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT37213.10915031041369No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT34622.892738074348883No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT24112.014555602904436No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT23281.9452034191462162No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT20151.6836704852146156No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT19071.5934290894810286No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT17541.4655871121917796No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC16711.3962349284335598No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT15851.3243760392382957No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT14141.1814938293267825No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC13511.1288530151488565No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT13341.1146483510056067No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC12451.0402827563732986No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC11790.9851352367583286No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC10200.8522798485949915No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC9810.8196926779134185No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT9790.818021540955389No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT9720.8121725616022861No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC9520.7954611920219922No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC8170.6826594473550079No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT7700.6433877288413172No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC7580.6333609070931407No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT7510.627511927740038No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC7090.5924180516214206No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC7060.5899113461843766No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC6870.5740355450830973No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC6420.5364349635274359No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6350.530585984174333No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6270.5239014363422154No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6070.5071900667619215No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC5690.47543846455936295No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC5470.45705595802103965No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC5380.44953584170990735No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC5360.44786470475187795No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC5200.43449560908764273No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5120.4278110612555252No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC4630.386868205783805No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4360.3643078568504082No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4190.3501031927071583No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC4170.34843205574912894No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4000.33422739160587905No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC3980.33255625464784966No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3980.33255625464784966No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3950.3300495492108056No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3950.3300495492108056No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3930.3283784122527762No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3920.32754284377376147No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3740.3125026111514969No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3370.2815865774279531No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3250.27155975567977675No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3210.26821748176371796No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC3000.2506705437044093No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2950.2464927013093358No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2650.22142564693889485No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2310.19301631865239516No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2260.18883847625732167No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2240.18716733929929227No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2200.18382506538323348No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2170.1813183599461894No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1810.15123789470166027No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1800.15040232622264557No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA1720.143717778390528No Hit
CCTACGGGTGGCTGNAGTGGGGAATATTGCACAATGGGCGCAAGCNTGAT1680.14037550447446923No Hit
CCTACGGGGGGCTGNAGTGGGGAATATTGCACAATGGGCGCAAGCNTGAT1510.12617084033121934No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1470.12282856641516056No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA1410.11781515554107236No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT1410.11781515554107236No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1400.11697958706205766No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1390.11614401858304298No Hit
CCTACGGGTGGCAGNAGTGGGGAATATTGCACAATGGGCGCAAGCNTGAT1380.11530845010402828No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1350.11280174466698417No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1340.11196617618796949No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCACAATGGGCGCAAGCCTGAT1290.107788333792896No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT1290.107788333792896No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1280.1069527653138813No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA1250.1044460598768372No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA1200.1002682174817637No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1200.1002682174817637No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)