FastQCFastQC Report
Wed 2 Aug 2023
EGAF00008120277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88556
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT67317.600840146348073No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT53896.085414878720809No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT50805.736483129319301No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT44585.034102714666426No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT36204.087808844121234No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT26372.9777767740187No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT25282.854690817110077No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT24192.7316048602014544No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT9841.1111612990649984No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT7690.8683770721351461No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT7310.8254663715614977No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT7130.8051402502371382No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC6850.773521839288134No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6610.7464203441889878No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT6400.7227065359772348No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC6110.6899588960657663No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5870.66285740096662No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5250.5928452052938253No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT5030.5680021681196079No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4920.5555806495324992No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4610.5205745516961019No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4560.5149284068837798No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4410.4979899724468133No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4400.49686074348434894No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4140.46750079046027376No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4110.46411310357288044No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3900.4403992953611274No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3880.4381408374361986No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3760.42459008988662544No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT3680.41555625818690994No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3660.41329780026198115No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3640.4110393423370522No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3560.4020055106373368No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3520.3974885947874791No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT3360.3794209313880483No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3290.37151632865079726No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3100.3500609783639731No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3060.34554406251411535No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2840.3207010253398979No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2800.3161841094900402No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2800.3161841094900402No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2760.31166719364018247No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2730.3082795067527892No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2720.3071502777903248No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2720.3071502777903248No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2670.3015041329780026No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2630.2969872171281449No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2600.2935995302407516No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2530.2856949275035006No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2520.28456569854103614No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2510.28343646957857177No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2430.2744026378788563No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2420.2732734089163919No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2390.26988572202899863No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2360.2664980351416053No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT2220.25068882966710326No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT2050.231491937305208No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT1880.21229504494331272No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1850.20890735805591942No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1810.2043904422060617No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1760.19874429739373956No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1670.1885812367315597No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1660.18745200776909526No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1590.17954740503184427No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1590.17954740503184427No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1530.17277203125705767No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1500.16938434436966437No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1440.16260897059487783No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT1400.1580920547450201No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1390.15696282578255566No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1370.1547043678576268No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1370.1547043678576268No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT1310.14792899408284024No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1270.14341207823298252No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1190.13437824653326708No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT1170.13211978860833823No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1150.12986133068340935No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT1040.11743981209630064No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC950.10727675143412077No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)