FastQCFastQC Report
Wed 2 Aug 2023
EGAF00008120282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67807
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG13411.9776719217779877No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG12251.8065981388352235No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG10451.5411388204757621No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG9481.3980857433598302No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG8671.2786290500980724No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG8511.2550326662438982No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG8291.2225876384444083No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6871.013169731738611No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5840.8612680106773636No Hit
GNNTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4590.6769212618166266No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4410.6503753299806805No Hit
GNNTANTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3910.5766366304363857No Hit
GNNTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3300.4866754169923459No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3260.48077632102880236No Hit
GNNTANCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3230.4763519990561446No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG2960.43653310130222545No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2830.41736103942070873No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG2830.41736103942070873No Hit
NNNTNNTGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG2720.4011385255209639No Hit
GNNTANCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2680.3952394295574203No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2670.3937646555665344No Hit
NNNTNNTCGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG2580.38049168964856134No Hit
GNNTANTCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG2570.3790169156576755No Hit
GANTANTGGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG2560.37754214166678957No Hit
GNNTANCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2520.37164304570324597No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2520.37164304570324597No Hit
GNNTANTGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG2520.37164304570324597No Hit
NANTANTGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG2470.3642691757488165No Hit
GNNTANAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2430.3583700797852729No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2400.3539457578126152No Hit
NNNTNNTAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG2310.34067279189464217No Hit
NNCTNNTGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG2270.33477369593109857No Hit
GANTANTCGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG2250.33182414794932674No Hit
NANTNNCGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG2240.3303493739584409No Hit
GNNTANACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2220.32739982597666906No Hit
GANTNNTCGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG2200.3244502779948973No Hit
NNNTNNTCGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG2160.3185511820313537No Hit
GANTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2140.31560163404958186No Hit
NNCTNNTCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG2130.314126860058696No Hit
GANTNNTGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG2100.3097025380860383No Hit
NNNTNNCGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG2100.3097025380860383No Hit
NNNTNNTGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG2090.3082277640951524No Hit
GANTANTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2000.29495479817717934No Hit
GANTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2000.29495479817717934No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1990.2934800241862935No Hit
NANTANTCGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG1980.2920052501954075No Hit
GNNTANCGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG1950.2875809282227499No Hit
NNCTNNTAGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG1910.2816818322592063No Hit
GANTANTAGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG1850.27283318831389086No Hit
NANTNNTAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG1850.27283318831389086No Hit
GNNTANAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1830.2698836403321191No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1820.2684088663412332No Hit
NANTNNCCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG1810.2669340923503473No Hit
NNCTNNCGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG1810.2669340923503473No Hit
GNNTANTAGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG1810.2669340923503473No Hit
NNNTNNCGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG1780.2625097703776896No Hit
NNNTNNTAGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG1770.2610349963868037No Hit
NANTANTAGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG1730.2551359004232601No Hit
NNNTNNCAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG1730.2551359004232601No Hit
GANTANCGGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG1730.2551359004232601No Hit
GANTNNTAGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG1690.24923680445971655No Hit
NANTNNAGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG1690.24923680445971655No Hit
GANTANCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1670.24628725647794478No Hit
GNNTANCCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG1660.24481248248705884No Hit
GANTNNCCGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG1650.24333770849617295No Hit
GACTACTGGGGTATCTAATCCTGTTTGACCCCCACGCTTTCGCACATCAG1650.24333770849617295No Hit
GANTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1610.23743861253262938No Hit
NANTANCGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG1610.23743861253262938No Hit
GANTNNCGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG1590.2344890645508576No Hit
GANTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1540.22711519459642807No Hit
NNCTNNAGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG1530.22564042060554218No Hit
GANTANCAGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG1510.2226908726237704No Hit
NNNTNNCCGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG1490.2197413246419986No Hit
GACTACTAGGGTATCTAATCCTGTTTGACCCCCACGCTTTCGCACATCAG1480.21826655065111272No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1480.21826655065111272No Hit
GANTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1450.213842228678455No Hit
GANTANCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1450.213842228678455No Hit
GNNTANCAGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG1420.20941790670579732No Hit
NANTNNCAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG1390.20499358473313967No Hit
NNNTNNAGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG1390.20499358473313967No Hit
GANTANAGGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG1370.20204403675136784No Hit
NNCTNNACGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG1370.20204403675136784No Hit
GANTANCCGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG1360.20056926276048195No Hit
GANTANCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1360.20056926276048195No Hit
NNNTNNAGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG1350.19909448876959607No Hit
NNCTNNCAGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG1350.19909448876959607No Hit
GACTACTCGGGTATCTAATCCTGTTTGACCCCCACGCTTTCGCACATCAG1340.19761971477871015No Hit
GANTNNCAGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG1330.19614494078782427No Hit
GANTANAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1310.19319539280605247No Hit
GNNTANAGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG1310.19319539280605247No Hit
NANTNNACGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG1300.19172061881516658No Hit
NNNTNNCCGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG1290.19024584482428067No Hit
GANTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1290.19024584482428067No Hit
NANTANCCGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG1270.18729629684250887No Hit
NNNTNNACGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG1250.18434674886073707No Hit
NNNTNNCAGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG1230.1813972008789653No Hit
GNNTANACGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG1230.1813972008789653No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1230.1813972008789653No Hit
NANTANAGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG1230.1813972008789653No Hit
GANTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1220.1799224268880794No Hit
NNCTNNCCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG1220.1799224268880794No Hit
NANTANCAGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG1200.1769728789063076No Hit
GACTACCGGGGTATCTAATCCTGTTTGACCCCCACGCTTTCGCACATCAG1190.1754981049154217No Hit
GNNTANAAGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG1170.1725485569336499No Hit
GANTNNAGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG1170.1725485569336499No Hit
GANTANACGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG1150.16959900895187813No Hit
GANTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1120.16517468697922044No Hit
GANTANACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1110.16369991298833453No Hit
GACTACCCGGGTATCTAATCCTGTTTGACCCCCACGCTTTCGCACATCAG1060.15632604303390504No Hit
GACTACCAGGGTATCTAATCCTGTTTGACCCCCACGCTTTCGCACATCAG1060.15632604303390504No Hit
NNNTNNAAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG1050.15485126904301916No Hit
GANTNNACGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG1040.15337649505213327No Hit
GNNTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1040.15337649505213327No Hit
NANTANAAGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG1020.15042694707036147No Hit
NNNTNNAAGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG1010.14895217307947556No Hit
NANTNNAAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG1010.14895217307947556No Hit
GACTACACGGGTATCTAATCCTGTTTGACCCCCACGCTTTCGCACATCAG1000.14747739908858967No Hit
NANTANACGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG1000.14747739908858967No Hit
GNNTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG990.14600262509770376No Hit
GANTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG990.14600262509770376No Hit
GANTANAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG990.14600262509770376No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG980.14452785110681787No Hit
GACTACAGGGGTATCTAATCCTGTTTGACCCCCACGCTTTCGCACATCAG980.14452785110681787No Hit
NNNTNNACGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG980.14452785110681787No Hit
GANTANAAGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG970.14305307711593196No Hit
GANTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG940.1386287551432743No Hit
NNCTNNAAGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG920.1356792071615025No Hit
GNNTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG910.1342044331706166No Hit
NNNTNNTGGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG880.1297801111979589No Hit
GANTNNAAGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG870.12830533720707302No Hit
GANTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG770.11355759729821403No Hit
NNNTNNTGGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG750.11060804931644225No Hit
GNNTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG730.10765850133467045No Hit
NNNTNNTCGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG720.10618372734378456No Hit
GNNTANTCGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG700.10323417936201278No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)