FastQCFastQC Report
Wed 8 May 2024
EGAF00008120284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130926
Total Bases39.4 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG41683.1834776896873045No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG38372.930663122679987No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG30492.3287964193513893No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG30472.3272688388860883No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG25601.9553029955852925No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG23691.809419061149046No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG23111.765119227655317No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG19831.5145960313459512No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG16841.2862227517834501No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9500.7256007210179797No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9020.6889387898507554No Hit
GNNTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7370.5629134014634221No Hit
GNNTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7210.550692757741014No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6170.4712585735453615No Hit
GNNTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6080.4643844614515069No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5870.4483448665658463No Hit
GNNTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5840.44605349586789483No Hit
GNNTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5760.43994317400669086No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5690.4345966423781373No Hit
NANTNNTCGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG5480.4185570474924767No Hit
NANTNNTGGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG5370.41015535493332106No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5130.391824389349709No Hit
GNNTANACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5000.38189511632525247No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4860.37120205306814535No Hit
GANTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4850.37043826283549486No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4790.3658555214395919No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4760.3635641507416403No Hit
GANTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4700.35898140934573725No Hit
NNNTNNTGGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG4650.35516245818248476No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4480.3421780242274262No Hit
NNNTNNTGGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG4460.34065044376212517No Hit
GANTNNTGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG4360.3330125414356201No Hit
NNCTNNTGGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG4340.3314849609703191No Hit
NANTANTGGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG4330.3307211707376686No Hit
NANTNNCGGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG4280.32690221957441606No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4270.32613842934176557No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4250.3246108488764646No Hit
GANTANTGGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG4240.323847058643814No Hit
GNNTANTCGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG4240.323847058643814No Hit
GANTNNTCGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG4200.32079189771321204No Hit
NNNTNNTCGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG4180.31926431724791104No Hit
NANTANTCGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG4170.31850052701526055No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4150.3169729465499595No Hit
GNNTANTGGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG4020.307043673525503No Hit
NNNTNNTCGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG4010.30627988329285244No Hit
GANTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3930.3001695614316484No Hit
NNCTNNTCGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG3920.2994057711989979No Hit
GNNTANAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3810.29100407863984235No Hit
NANTNNTAGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG3740.2856575470112888No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3700.28260238608068683No Hit
NNNTNNCGGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG3680.28107480561538584No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3640.2780196446847838No Hit
GANTANTCGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG3620.27649206421948275No Hit
NNNTNNCGGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG3580.27343690328888076No Hit
GANTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3420.26121625956647265No Hit
NNCTNNCGGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG3400.25968867910117166No Hit
NANTNNCCGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG3350.2558697279379191No Hit
NNNTNNTAGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG3330.25434214747261813No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3310.25281456700731714No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3290.2512869865420161No Hit
GNNTANCGGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG3290.2512869865420161No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3280.2505231963093656No Hit
GANTNNTAGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG3250.24823182561141405No Hit
GNNTANTAGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG3180.24288529398286055No Hit
NNCTNNTAGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG3160.24135771351755952No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3150.24059392328490906No Hit
NANTNNAGGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG3140.23983013305225853No Hit
NANTANTAGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG3130.239066342819608No Hit
NNNTNNTAGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG3100.2367749721216565No Hit
GANTANCGGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG3090.23601118188900602No Hit
NANTANCGGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG3080.2352473916563555No Hit
GANTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3050.23295602095840398No Hit
NNNTNNCCGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG3040.23219223072575346No Hit
GANTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3040.23219223072575346No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3030.231428440493103No Hit
GANTNNCGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG3010.22990086002780194No Hit
GANTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2930.22379053816659794No Hit
NANTNNCAGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG2930.22379053816659794No Hit
NNNTNNCCGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG2910.22226295770129695No Hit
GANTANTAGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG2910.22226295770129695No Hit
GNNTANCCGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG2880.21997158700334543No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2850.21768021630539386No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2850.21768021630539386No Hit
NANTANCCGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG2730.20851473351358782No Hit
GANTNNCCGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG2710.20698715304828683No Hit
NANTNNACGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG2690.20545957258298583No Hit
GNNTANCAGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG2680.2046957823503353No Hit
NNNTNNACGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG2680.2046957823503353No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2640.20164062141973327No Hit
NNCTNNAGGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG2640.20164062141973327No Hit
GANTANCCGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG2640.20164062141973327No Hit
NANTANAGGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG2620.20011304095443228No Hit
NNNTNNCAGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG2620.20011304095443228No Hit
GNNTANAGGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG2590.19782167025648076No Hit
NNCTNNCCGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG2590.19782167025648076No Hit
NNCTNNCAGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG2570.19629408979117977No Hit
GANTANACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2570.19629408979117977No Hit
NNNTNNAGGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG2500.19094755816262624No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2470.18865618746467472No Hit
NNNTNNCAGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG2460.1878923972320242No Hit
NANTNNAAGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG2450.1871286069993737No Hit
GANTNNCAGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG2450.1871286069993737No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2440.1863648167667232No Hit
GANTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2440.1863648167667232No Hit
NNNTNNAGGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG2430.18560102653407268No Hit
GANTANCAGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG2410.18407344606877168No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2370.18101828513816964No Hit
GANTNNAGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG2290.17490796327696562No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2250.1718528023463636No Hit
GANTANACGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG2190.16727006095046057No Hit
NANTANCAGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG2170.16574248048515955No Hit
GANTANAGGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG2140.16345110978720803No Hit
GANTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2140.16345110978720803No Hit
GANTNNACGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG2080.15886836839130503No Hit
NNCTNNACGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG2080.15886836839130503No Hit
GNNTANACGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG2060.157340787926004No Hit
NNNTNNAAGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG2050.1565769976933535No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2000.15275804653010097No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1960.14970288559949896No Hit
GNNTANAAGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG1930.14741151490154744No Hit
NNCTNNAAGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG1920.14664772466889692No Hit
GANTANAAGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG1920.14664772466889692No Hit
NNNTNNAAGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG1900.14512014420359592No Hit
NNNTNNACGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG1860.1420649832729939No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1840.14053740280769292No Hit
NANTANAAGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG1730.13213571024853735No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1700.12984433955058583No Hit
NANTANACGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG1700.12984433955058583No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1590.12144264699143027No Hit
GANTNNAAGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG1510.11533232513022623No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1450.11074958373432321No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1430.10922200326902219No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)