FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008120286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120815
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG32632.700823573231801No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG29842.4698919836113062No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG23771.9674709266233499No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG22391.8532466994992343No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG20431.6910151885113602No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG18691.5469933369200843No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG18101.4981583412655712No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG15301.2663990398543228No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG13381.1074783760294666No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7760.6423043496254605No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7410.6133344369490543No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6610.5471174936886976No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6150.5090427513139925No Hit
GNNTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5930.4908330919173943No Hit
GNNTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5370.44448123163514464No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4950.40971733642345737No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4890.4047510656789306No Hit
GNNTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4830.3997847949344039No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4570.37826428837478787No Hit
NANTNNTGGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG4510.3732980176302611No Hit
GNNTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4400.3641931879319621No Hit
GANTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4040.33439556346480154No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4030.3335678516740471No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3980.3294292927202748No Hit
NANTNNTCGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG3900.3228075983942391No Hit
GNNTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3880.32115217481273023No Hit
NNNTNNTGGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG3810.315358192277449No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3790.31370276869594005No Hit
GANTNNTGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG3780.3128750569051856No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3730.3087364979514133No Hit
NANTANTGGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG3690.3054256507883955No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3620.29963166825311427No Hit
GNNTANACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3600.29797624467160533No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3570.29549310929934197No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3560.2946653975085875No Hit
NNNTNNTGGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG3550.29383768571783303No Hit
NNCTNNTGGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG3500.28969912676406073No Hit
GNNTANTGGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG3490.28887141497330626No Hit
NNNTNNTCGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG3440.28473285601953396No Hit
NNCTNNTCGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG3410.2822497206472706No Hit
NNNTNNTCGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG3350.2772834499027439No Hit
GANTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3350.2772834499027439No Hit
GANTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3290.2723171791582171No Hit
GNNTANTCGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG3290.2723171791582171No Hit
GANTNNTCGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG3290.2723171791582171No Hit
GANTANTGGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG3220.2665231966229359No Hit
NANTNNCGGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG3220.2665231966229359No Hit
NANTANTCGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG3210.2656954848321814No Hit
GANTANTCGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG3150.26072921408765465No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3140.25990150229690023No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3090.25576294334312794No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2970.24583040185407443No Hit
GNNTANAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2960.24500269006331996No Hit
NNNTNNCGGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG2960.24500269006331996No Hit
NANTNNTAGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG2860.23672557215577536No Hit
GANTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2820.2334147249927575No Hit
NNNTNNCGGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG2800.23175930141124862No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2770.2292761660389852No Hit
NANTNNCCGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG2680.2218267599221951No Hit
GANTNNCGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG2660.22017133634068617No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2630.2176882009684228No Hit
NNCTNNTAGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG2620.21686048917766834No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2590.21437735380540496No Hit
GANTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2580.2135496420146505No Hit
NNNTNNTAGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG2580.2135496420146505No Hit
NNCTNNCGGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG2570.21272193022389604No Hit
NANTNNAGGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG2540.21023879485163266No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2520.20858337127012375No Hit
NNNTNNTAGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG2510.2077556594793693No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2500.20692794768861483No Hit
GNNTANTAGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG2480.2052725241071059No Hit
GNNTANCGGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG2480.2052725241071059No Hit
NANTNNCAGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG2460.203617100525597No Hit
NANTANCGGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG2440.20196167694408804No Hit
NNNTNNCAGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG2440.20196167694408804No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2430.2011339651533336No Hit
NANTANTAGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG2430.2011339651533336No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2410.1994785415718247No Hit
GANTANTAGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG2400.19865082978107024No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2370.19616769440880685No Hit
NANTANCCGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG2300.19037371187352564No Hit
NNNTNNCCGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG2300.19037371187352564No Hit
GANTANCGGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG2270.18789057650126226No Hit
GNNTANCCGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG2250.18623515291975334No Hit
NNNTNNCCGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG2220.18375201754748996No Hit
NNNTNNCAGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG2220.18375201754748996No Hit
GANTANCCGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG2200.18209659396598105No Hit
GANTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2190.18126888217522658No Hit
NNCTNNCCGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG2170.17961345859371766No Hit
GANTNNCCGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG2170.17961345859371766No Hit
NNNTNNAGGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG2140.17713032322145428No Hit
GANTNNCAGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG2130.17630261143069983No Hit
GANTANACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2100.17381947605843645No Hit
GANTNNTAGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG2090.17299176426768198No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2060.1705086288954186No Hit
NNNTNNAGGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG2030.16802549352315524No Hit
NNCTNNAGGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG2030.16802549352315524No Hit
NNNTNNACGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG2020.16719778173240077No Hit
GANTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1930.15974837561561064No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1930.15974837561561064No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1910.15809295203410173No Hit
NNCTNNCAGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG1880.15560981666183835No Hit
GANTNNAGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG1870.1547821048710839No Hit
GNNTANAGGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG1850.15312668128957496No Hit
NNNTNNAAGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG1830.15147125770806605No Hit
GANTANAGGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG1820.1506435459173116No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1810.14981583412655713No Hit
NNNTNNACGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG1800.14898812233580266No Hit
NANTNNACGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG1790.14816041054504822No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1730.14319413980052148No Hit
GNNTANAAGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG1720.14236642800976698No Hit
NANTANCAGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG1720.14236642800976698No Hit
GANTANCAGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG1710.14153871621901254No Hit
NANTNNAAGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG1700.1407110044282581No Hit
NNCTNNACGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG1700.1407110044282581No Hit
GNNTANCAGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG1660.13740015726524024No Hit
GANTANACGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG1660.13740015726524024No Hit
GANTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1650.1365724454744858No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1640.13574473368373133No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1630.13491702189297686No Hit
GNNTANACGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG1630.13491702189297686No Hit
NANTANAGGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG1600.1324338865207135No Hit
GANTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1600.1324338865207135No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1600.1324338865207135No Hit
NNNTNNAAGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG1580.13077846293920456No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1570.12995075114845012No Hit
GNNTANTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1560.12912303935769565No Hit
GANTANAAGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG1530.12663990398543226No Hit
GNNTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1530.12663990398543226No Hit
GNNTANCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1420.11753507428713322No Hit
NNCTNNAAGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG1400.11587965070562431No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1390.11505193891486985No Hit
GANTNNACGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG1370.11339651533336093No Hit
GANTNNAAGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG1370.11339651533336093No Hit
GNNTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1350.111741091751852No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1300.1076025327980797No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1270.10511939742581633No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1270.10511939742581633No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1240.10263626205355295No Hit
NANTANAAGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG1230.1018085502627985No Hit
NANTANACGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG1230.1018085502627985No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)