FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008120288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133610
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG47213.5334181573235535No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG44103.3006511488661032No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG35702.6719556919392264No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG33502.507297357982187No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG29242.1884589476835568No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG26782.0043409924406856No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG25981.944465234638126No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG22911.714692014070803No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG20141.50737220267944No Hit
GNNTANTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG11070.8285307985929197No Hit
GNNTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG10720.8023351545542998No Hit
GNNTANCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG8450.6324376917895367No Hit
GNNTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG7990.5980091310530649No Hit
GNNTANCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6790.5081954943492254No Hit
GNNTANCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6740.5044532594865654No Hit
GNNTANAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6480.4849936382007335No Hit
GANTANTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6410.4797545093930095No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG6030.4513135244367936No Hit
GANTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5990.4483197365466656No Hit
GANTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5950.4453259486565377No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG5930.4438290547114737No Hit
GNNTANACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5910.4423321607664097No Hit
GANTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5780.43260235012349374No Hit
GANTNNTGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG5470.40940049397500183No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG5290.39592844846942593No Hit
NNNTNNTGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG5260.39368310755183No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG5190.38844397874410597No Hit
NNCTNNTGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG5190.38844397874410597No Hit
NNNTNNTGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG5170.386947084799042No Hit
GANTANTGGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG5150.385450190853978No Hit
NNNTNNTCGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG5120.383204849936382No Hit
GNNTANTCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG5100.381707955991318No Hit
GANTANCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5070.37946261507372203No Hit
GNNTANTGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG5030.37646882718359403No Hit
NNCTNNTCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG4990.3734750392934661No Hit
GANTNNTCGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG4970.37197814534840207No Hit
NANTANTGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG4880.36524212259561406No Hit
NANTNNCGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG4880.36524212259561406No Hit
NNNTNNTCGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG4800.3592545468153581No Hit
GANTANTCGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG4790.3585060998428261No Hit
NANTANTCGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG4730.35401541800763414No Hit
GANTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4720.35326697103510213No Hit
GNNTANAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4600.3442856073647182No Hit
NANTNNTAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG4420.3308135618591423No Hit
GANTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4270.3195868572711624No Hit
GANTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4250.31808996332609835No Hit
NNNTNNCGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG4090.3061148117655864No Hit
GANTANAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4020.30087568295786243No Hit
NNCTNNCGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG4000.2993787890127984No Hit
NANTNNCCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG3930.2941396602050744No Hit
GANTNNCGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG3910.29264276626001046No Hit
NNNTNNTAGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG3900.2918943192874785No Hit
NNNTNNCGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG3860.2889005313973505No Hit
NANTANCGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG3850.2881520844248185No Hit
NANTNNCAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG3830.2866551904797545No Hit
NNCTNNTAGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG3690.27617693286430656No Hit
GANTANCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3690.27617693286430656No Hit
GNNTANCGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG3690.27617693286430656No Hit
GANTANTAGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG3620.2709378040565826No Hit
GANTNNTAGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG3620.2709378040565826No Hit
GANTANCGGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG3610.27018935708405056No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG3580.26794401616645463No Hit
GANTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3550.26569867524885865No Hit
GNNTANTAGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG3530.2642017813037946No Hit
NNNTNNCCGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG3510.26270488735873065No Hit
NNNTNNTAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG3490.26120799341366663No Hit
NANTNNAGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG3430.25671731157847466No Hit
NNNTNNCAGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG3370.2522266297432827No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG3340.2499812888256867No Hit
GNNTANCCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG3330.2492328418531547No Hit
GANTANCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3320.2484843948806227No Hit
NANTANTAGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG3300.24698750093555874No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3270.24474216001796273No Hit
NNCTNNCCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG3270.24474216001796273No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG3210.24025147818277073No Hit
GANTANCCGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG3200.23950303121023875No Hit
GANTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3200.23950303121023875No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG3190.23875458423770674No Hit
GANTANACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3120.23351545542998278No Hit
NANTANCCGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG3110.2327670084574508No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG3100.23201856148491878No Hit
GNNTANCAGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG3070.22977322056732283No Hit
GANTANAGGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG3040.22752787964972682No Hit
NNNTNNCCGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG3010.22528253873213083No Hit
GANTNNCCGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG3000.22453409175959885No Hit
GANTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2990.22378564478706683No Hit
NNNTNNAGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG2920.21854651597934285No Hit
NNCTNNAGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG2920.21854651597934285No Hit
NNCTNNCAGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG2910.21779806900681084No Hit
GANTNNCAGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG2900.21704962203427888No Hit
NANTNNACGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG2880.21555272808921488No Hit
NNNTNNCAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG2870.21480428111668287No Hit
GNNTANAGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG2820.2110620462540229No Hit
NANTANCAGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG2820.2110620462540229No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2770.2073198113913629No Hit
GANTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2740.20507447047376695No Hit
GANTNNAGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG2720.20357757652870295No Hit
NNNTNNACGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG2710.20282912955617094No Hit
NNNTNNACGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG2620.196093106803383No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG2610.19534465983085098No Hit
GANTANCAGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG2600.194596212858319No Hit
GNNTANACGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG2590.19384776588578698No Hit
GANTANAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2570.192350871940723No Hit
NANTNNAAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG2530.18935708405059504No Hit
NNCTNNACGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG2530.18935708405059504No Hit
GANTANACGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG2470.18486640221540304No Hit
NNNTNNAGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG2450.18336950827033904No Hit
NNNTNNAAGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG2380.1781303794626151No Hit
NANTANAGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG2370.17738193249008308No Hit
GANTNNACGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG2300.1721428036823591No Hit
GANTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2300.1721428036823591No Hit
GANTANAAGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG2290.1713943567098271No Hit
GNNTANAAGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG2270.1698974627647631No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2230.16690367487463514No Hit
NANTANAAGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG2210.16540678092957115No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG2180.16316144001197516No Hit
GANTNNAAGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG2150.16091609909437915No Hit
NANTANACGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG2100.1571738642317192No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2000.1496893945063992No Hit
NNCTNNAAGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG1990.14894094753386722No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1960.14669560661627123No Hit
NNNTNNAAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG1860.13921113689095127No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1780.1332235611106953No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1760.1317266671656313No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1710.12798443230297132No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1670.12499064441284335No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG1460.10927325798967143No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1450.10852481101713944No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1390.10403412918194746No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)