FastQCFastQC Report
Wed 2 Aug 2023
EGAF00008120299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87279
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT19532.237651668786306No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT19062.1838013726096768No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT14191.6258206441412022No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT14181.624674893158721No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT14101.615508885298869No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT13221.5146827988405No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT12841.4711442615062043No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT12501.4321887281018344No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT12021.377192680942724No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT10851.2431398159923923No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT10731.2293908042026145No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT9851.1285647177442455No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC9341.0701314176376906No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC9221.056382405847913No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT9031.0346131371807652No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT8841.0128438685136172No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7740.8868112604406558No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7410.8490014780187674No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT6940.7951511818421384No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC6810.7802564190698793No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6790.7779649171049164No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6780.776819166122435No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC6770.7756734151399535No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC6600.7561956484377685No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC6410.7344263797706206No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC6210.7115113601209913No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT5890.6748473286815844No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC5720.6553695619793994No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5510.6313087913472886No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5420.6209970325049554No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4920.563709483380882No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC4580.524753949976512No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT4430.5075676852392901No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4270.4892356695195866No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4270.4892356695195866No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3830.43882262629040203No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3810.43653112432543906No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3640.41705335762325413No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3560.40788734976340246No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3530.404450096815958No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3440.3941383379736248No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3440.3941383379736248No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC3410.39070108502618045No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3380.387263832078736No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3330.3815350771663287No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3300.37809782421888427No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3280.37580632225392135No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3220.36893181635903255No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC3090.35403705358677345No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC2960.33914229081451436No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2860.3276847809896997No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC2630.30133250839262593No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2580.2956037534802186No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2520.2887292475853298No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2300.26352272597073756No Hit
CCTACGGGTGGCTGCAGGCTTGGACTCTACTTTTTGTCATCCTTCCATCT2280.2612312240057746No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2140.245190710251034No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1950.22342144158388616No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1830.20967242979410852No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1820.20852667881162706No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC1780.20394367488170123No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1750.2005064219342568No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1740.19936067095177534No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1740.19936067095177534No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1740.19936067095177534No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1680.19248616505688654No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1660.1901946630919236No Hit
CCTACGGGGGGCTGCAGGCTTGGACTCTACTTTTTGTCATCCTTCCATCT1660.1901946630919236No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC1640.18790316112696068No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1630.1867574101444792No Hit
CCTACGGGTGGCAGCAGGCTTGGACTCTACTTTTTGTCATCCTTCCATCT1560.17873715326710893No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1560.17873715326710893No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1480.16957114540725718No Hit
CCTACGGGGGGCAGCAGGCTTGGACTCTACTTTTTGTCATCCTTCCATCT1450.1661338924598128No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1430.16384239049484986No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1430.16384239049484986No Hit
CCTACGGGCGGCTGCAGGCTTGGACTCTACTTTTTGTCATCCTTCCATCT1420.16269663951236837No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1280.14665612575762785No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1240.14207312182770196No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1230.1409273708452205No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1230.1409273708452205No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1220.13978161986273901No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1210.13863586888025756No Hit
CCTACGGGTGGCTGCAGTGGCGAAGGCGGATTGCTGGACGACAACTGACG1150.13176136298536878No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1150.13176136298536878No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1110.1271783590554429No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1100.1260326080729614No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT1100.1260326080729614No Hit
CCTACGGGCGGCAGCAGGCTTGGACTCTACTTTTTGTCATCCTTCCATCT1090.12488685709047996No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1080.12374110610799849No Hit
CCTACGGGTGGCTGCAGTAGCGAAGGCGGCTTTCTGGACTGTAACTGACG1040.11915810217807263No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC990.11342934726566527No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC980.11228359628318381No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC970.11113784530070235No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC950.1088463433357394No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGCACAATGGGGGAAACCCTGAT950.1088463433357394No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT940.10770059235325795No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA940.10770059235325795No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC920.10540909038829499No Hit
CCTACGGGTGGCTGCAGTGGCGAAGGCGGCTTTCTGGACGACAACTGACG920.10540909038829499No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT920.10540909038829499No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC910.10426333940581353No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA890.10197183744085062No Hit
CCTACGGGAGGCAGCAGGCTTGGACTCTACTTTTTGTCATCCTTCCATCT880.10082608645836913No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)