FastQCFastQC Report
Wed 8 May 2024
EGAF00008120302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74662
Total Bases22.4 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG7761.0393506737028209No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG7641.0232782406043235No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7120.9536310305108354No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7100.950952291661086No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG6050.8103185020492352No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5910.7915673301009886No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5380.7205807505826256No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5210.6978114703597547No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5180.6937933620851303No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4970.6656666041627601No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4780.6402185850901396No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4400.5893225469448984No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4260.5705713749966516No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4200.562535158447403No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4100.5491414641986553No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3870.5183359674265355No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3640.48753047065441585No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3410.45672497388229616No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3380.4527068656076719No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3290.44065254078379895No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2560.34287857276794087No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2360.31609118427044547No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG2300.30805496772119684No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2150.2879644263480753No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2040.27323136267445286No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1980.2651951461252043No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1980.2651951461252043No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG1930.25849829900083043No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1880.25180145187645653No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1870.2504620824515818No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1840.24644397417695746No Hit
GNNTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1700.22769280222871072No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1620.21697784682971258No Hit
GNNTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1570.2102809997053387No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG1540.2062628914307144No Hit
GNNTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1500.20090541373121534No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1480.1982266748814658No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1440.1928691971819667No Hit
GNNTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1410.18885108890734242No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1400.18751171948246764No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG1390.1861723500575929No Hit
NANTNNTCGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGAGCCTCAG1330.17813613350834429No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG1290.1727786558088452No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG1280.17143928638397044No Hit
GNNTANACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1230.16474243925959658No Hit
GNNTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1220.16340306983472183No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1220.16340306983472183No Hit
GNNTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1210.16206370040984702No Hit
NANTNNTGGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGAGCCTCAG1210.16206370040984702No Hit
GNNTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1170.15670622271034795No Hit
GNNTANTGGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGAGCCTNNG1170.15670622271034795No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG1170.15670622271034795No Hit
GNNTANTCGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGAGCCTNNG1170.15670622271034795No Hit
NNNTNNTCGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG1160.1553668532854732No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1160.1553668532854732No Hit
NNNTNNTCGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG1150.15402748386059842No Hit
GNNTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1150.15402748386059842No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG1150.15402748386059842No Hit
GNNTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1140.15268811443572367No Hit
GANTNNTGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGAGCCNCAG1140.15268811443572367No Hit
GANTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1120.15000937558597413No Hit
GANTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1120.15000937558597413No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1110.14867000616109935No Hit
GNNTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1110.14867000616109935No Hit
GANTANTGGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG1100.1473306367362246No Hit
NNNTNNTGGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGAGCCNNNG1100.1473306367362246No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1090.14599126731134981No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1080.14465189788647503No Hit
NNCTNNTGGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGAGCCTCAG1080.14465189788647503No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG1070.14331252846160028No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1060.1419731590367255No Hit
NANTANTCGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGAGCCTCAG1050.14063378961185075No Hit
GANTANTGGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGAGCCTCAG1050.14063378961185075No Hit
NNCTNNTGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG1050.14063378961185075No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG1050.14063378961185075No Hit
GANTANTCGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG1050.14063378961185075No Hit
GANTNNTCGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGAGCCNCAG1040.13929442018697596No Hit
GNNTANAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1030.1379550507621012No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG1030.1379550507621012No Hit
GANTNNCGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGAGCCNCAG1030.1379550507621012No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1010.13527631191235168No Hit
GANTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1010.13527631191235168No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG1010.13527631191235168No Hit
GANTANTCGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGAGCCTCAG1010.13527631191235168No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1000.1339369424874769No Hit
NNCTNNTCGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGAGCCTCAG1000.1339369424874769No Hit
NNNTNNTAGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGAGCCNNNG990.13259757306260214No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG990.13259757306260214No Hit
GNNTANTCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG990.13259757306260214No Hit
NANTANTGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG980.13125820363772736No Hit
NNCTNNTCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG980.13125820363772736No Hit
NNNTNNTCGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGAGCCTCAG980.13125820363772736No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG970.1299188342128526No Hit
NNNTNNTCGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGAGCCNNNG960.12857946478797783No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG950.12724009536310307No Hit
NANTANTGGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGAGCCTCAG950.12724009536310307No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG940.12590072593822826No Hit
GANTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG930.12456135651335351No Hit
NANTNNCGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG930.12456135651335351No Hit
NANTNNCGGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGAGCCTCAG930.12456135651335351No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG920.12322198708847873No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG920.12322198708847873No Hit
NNNTNNCGGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGAGCCNNNG910.12188261766360396No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG910.12188261766360396No Hit
NNNTNNTGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG900.1205432482387292No Hit
NNNTNNTGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG890.11920387881385443No Hit
NNCTNNTAGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG890.11920387881385443No Hit
NNCTNNTAGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGAGCCTCAG890.11920387881385443No Hit
NNNTNNCGGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGAGCCTCAG880.11786450938897966No Hit
GNNTANTGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG880.11786450938897966No Hit
NNNTNNTAGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGAGCCTCAG870.11652513996410489No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG870.11652513996410489No Hit
NNNTNNTGGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGAGCCTCAG860.11518577053923013No Hit
GNNTANCGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG860.11518577053923013No Hit
NANTNNTAGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGAGCCTCAG850.11384640111435536No Hit
NNCTNNCGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG850.11384640111435536No Hit
GNNTANTAGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGAGCCTNNG850.11384640111435536No Hit
GNNTANCGGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGAGCCTNNG840.11250703168948059No Hit
GANTNNCCGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGAGCCNCAG840.11250703168948059No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG840.11250703168948059No Hit
GNNTANCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG840.11250703168948059No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG820.10982829283973106No Hit
NANTNNCAGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGAGCCTCAG820.10982829283973106No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG820.10982829283973106No Hit
GNNTANCCGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGAGCCTNNG820.10982829283973106No Hit
GANTNNTCGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG820.10982829283973106No Hit
GANTANTAGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG810.10848892341485629No Hit
GANTANTAGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGAGCCTCAG810.10848892341485629No Hit
GNNTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG800.10714955398998152No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG800.10714955398998152No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG800.10714955398998152No Hit
NANTANCGGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGAGCCTCAG800.10714955398998152No Hit
GANTNNCGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG790.10581018456510675No Hit
NANTNNTAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG790.10581018456510675No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG790.10581018456510675No Hit
GANTNNTGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG780.10447081514023199No Hit
NNNTNNCCGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGAGCCNNNG780.10447081514023199No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG780.10447081514023199No Hit
NANTANTCGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG780.10447081514023199No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG770.1031314457153572No Hit
GANTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG770.1031314457153572No Hit
GANTNNTAGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGAGCCNCAG770.1031314457153572No Hit
NNCTNNCCGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGAGCCTCAG760.10179207629048244No Hit
NANTANCCGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGAGCCTCAG760.10179207629048244No Hit
GANTANCGGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG760.10179207629048244No Hit
NNNTNNTAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG760.10179207629048244No Hit
GANTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG750.10045270686560767No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG750.10045270686560767No Hit
NANTNNCAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG750.10045270686560767No Hit
GANTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG750.10045270686560767No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA750.10045270686560767No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)