FastQCFastQC Report
Wed 8 May 2024
EGAF00008120307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122834
Total Bases36.9 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC59944.87975641923246No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC43843.5690444013872376No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC40633.307716104661576No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC37223.030105671068271No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC30242.461859094387547No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT29852.4301089274956444No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC22791.855349496067864No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT21951.7869645212237655No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT21011.7104384779458457No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC20681.6835729521142355No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT20521.6705472426201216No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT18651.518309262907664No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT15461.258609179868766No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT14841.2081345555790741No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT13551.10311477278278No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT13331.0852044222283732No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT12291.0005373105166322No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT11600.9443639383232656No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC11360.9248253740820946No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT11350.9240112672387123No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT9990.8132927365387433No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT9730.792125958610808No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT9650.785613103863751No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT8570.6976895647784815No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT8480.6903626031880424No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT8110.6602406499829038No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7800.6350033378380578No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC7650.622791735187326No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT7570.616278880440269No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7370.5999967435726266No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT7100.5780158588013091No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT6950.5658042561505772No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6720.5470797987527883No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6370.5185860592344139No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT6230.5071885634270642No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5920.4819512512822183No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5850.47625250337854336No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5780.47055375547486855No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5610.4567139391373724No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5380.43798948173958346No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT5120.4168227038116482No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5090.4143803832815019No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4740.3858866437631275No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT4570.37204682742563133No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4460.3630916521484279No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4040.3288991647263787No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3970.32320041682270384No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3950.32157220313593954No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3800.3093606004852077No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3490.2841232883403618No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGGACAATGGGGGCAACCCTGAT3280.26702704462933713No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCGATGGGCGAAAGCCTGAC3080.25074490776169467No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3050.24830258723154827No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3030.24667437354478403No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2730.22225116824332022No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCGATGGGCGAAAGCCTGAC2620.21329599296611687No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGGACAATGGGGGCAACCCTGAT2570.20922545874920623No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2520.2051549245322956No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTCCCGCAATGGGCGAAAGCCTGAC2310.188058680821271No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2280.1856163602911246No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2270.18480225344774248No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGGACAATGGGGGCAACCCTGAT2180.17747529185730335No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2150.17503297132715698No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGGACAATGGGGGCAACCCTGAT2130.17340475764039273No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2120.1725906507970106No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCGATGGGCGAAAGCCTGAC2050.16689190289333572No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2030.16526368920657147No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2010.16363547551980723No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCGATGGGCGAAAGCCTGAC1930.15712262077275022No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1820.14816744549554683No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGTGAACCCTGAT1810.1473533386521647No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTCCCGCAATGGGCGAAAGCCTGAC1760.1432828044352541No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCGATGGGCGAAAGCCTGAC1750.14246869759187197No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGGACAATGGGGGCAACCCTGAT1720.14002637706172558No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGACTAACTGGACTGTAACTGACG1640.13351352231466856No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTCCCGCAATGGGCGAAAGCCTGAC1620.13188530862790432No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGGT1610.1310712017845222No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATTGGGGGAACCCTGAT1570.1278147744109937No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1560.12700066756761158No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1550.12618656072422946No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1460.11885959913379032No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGTACAATGGGGGAAACCCTGAT1460.11885959913379032No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1460.11885959913379032No Hit
CCTACGGGGGGCAGCAGTGGGGAAGGCGACTAACTGGACTGTAACTGACG1450.1180454922904082No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1360.11071853069996905No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1350.10990442385658693No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCGATGGGCGAAAGCCTGAC1320.10746210332644056No Hit
CCTACGGGGGGCTGCAGTGGGGAAGGCGACTAACTGGACTGTAACTGACG1260.1025774622661478No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCATCAGATGTGTTCGAATGGGAT1260.1025774622661478No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCGATGGGCGAAAGCCTGAC1250.10176335542276568No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTGGACAATGGGGGCAACCCTGAT1240.10094924857938356No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)