FastQCFastQC Report
Wed 8 May 2024
EGAF00008120308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122834
Total Bases36.9 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG16891.37502645847241No Hit
GACTACTCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG14821.20650634189231No Hit
GACTACTAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG12311.0021655242033964No Hit
GACTACCGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG11860.9655307162512008No Hit
GACTACCCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG9940.8092222023218328No Hit
GACTACAGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG9270.7546770438152303No Hit
GACTACCAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG9040.7359525864174414No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG8120.6610547568262859No Hit
GACTACACGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG7940.6464008336454077No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG7290.5934838888255695No Hit
GACTACAAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG7050.5739453245843984No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG6070.4941628539329501No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG5890.47950893075207185No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5560.4526434049204618No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5530.45020108439031536No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4980.4054252080042985No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4590.37367504111239563No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4590.37367504111239563No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4560.3712327205822492No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4190.3411107673771106No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4000.3256427373528502No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3940.3207580962925574No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3860.31424524154550043No Hit
GNNTANTGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG3860.31424524154550043No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3730.3036618525815328No Hit
GNNTANTCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG3670.29877721152124004No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3220.2621424035690444No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2840.23120634352052366No Hit
GNNTANCGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG2680.2181806340264096No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2630.214110099809499No Hit
GNNTANCCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG2560.2084113519058241No Hit
GNNTANTAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG2550.20759724506244198No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2320.18887278766465312No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2260.18398814660436036No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2230.18154582607421396No Hit
GANTANTGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG2180.17747529185730335No Hit
GNNTANAGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG2060.16770600973671784No Hit
GANTACTGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1970.1603790481462787No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1960.15956494130289658No Hit
NANTNNTCGGGTNTNTNNTCNCATNTGNTCNCCNAGCNTTCGTCTCTCAG1930.15712262077275022No Hit
GNNTANCAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1920.1563085139293681No Hit
NANTNNTGGGGTNTNTNNTCNCATNTGNTCNCCNAGCNTTCGTCTCTCAG1900.15468030024260385No Hit
GNNTANACGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1870.15223797971245745No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1870.15223797971245745No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1860.15142387286907533No Hit
NNNTNNTGGGNNNTNTNATNNCATTTGNTCNCCNAGNTTTCGTCTCNNNG1850.1506097660256932No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1840.14979565918231108No Hit
GANTACTCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1820.14816744549554683No Hit
GANTANTCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1810.1473533386521647No Hit
GNNTANTCGGGTNTNTNNTNNCATTTGNTCNCNNAGNTTTCGTCTCTNNG1800.1465392318087826No Hit
NNCTNNTGGGGTATNTANTCNCATTTGNTCNCCNAGCTTTCGTCTCTCAG1750.14246869759187197No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1750.14246869759187197No Hit
GNNTANTGGGGTNTNTNNTNNCATTTGNTCNCNNAGNTTTCGTCTCTNNG1700.13839816337496133No Hit
NANTANTGGGGTATNTANTNNCATTTGNTCNCCNNGNTTTCGTCTCTCAG1680.13676994968819708No Hit
GANTANTGGGGTNTCTNNTNNCATTTGCTCCCCTAGNTTTCGTCTCTCAG1670.13595584284481493No Hit
NNNTNNTCGGNNNTNTNATNNCATTTGNTCNCCNAGNTTTCGTCTCNNNG1660.1351417360014328No Hit
GNNTANAAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1590.12944298809775795No Hit
GNNTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1580.12862888125437583No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1570.1278147744109937No Hit
NNCTNNTCGGGTATNTANTCNCATTTGNTCNCCNAGCTTTCGTCTCTCAG1550.12618656072422946No Hit
NANTNNCGGGGTNTNTNNTCNCATNTGNTCNCCNAGCNTTCGTCTCTCAG1540.12537245388084733No Hit
NNNTNNTGGGGTATNTNNTNNCATTTGNTCNCCNAGNTTTCGTCTCTCAG1530.1245583470374652No Hit
GANTANTAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1530.1245583470374652No Hit
GANTNNTGGGGTATNTAATNNCATTTGNTCNCCNNGCTTTCGTCTCNCAG1500.12211602650731883No Hit
NANTNNTAGGGTNTNTNNTCNCATNTGNTCNCCNAGCNTTCGTCTCTCAG1490.12130191966393669No Hit
GANTANTCGGGTNTCTNNTNNCATTTGCTCCCCTAGNTTTCGTCTCTCAG1460.11885959913379032No Hit
GANTACCGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1450.1180454922904082No Hit
GANTNNTCGGGTATNTAATNNCATTTGNTCNCCNNGCTTTCGTCTCNCAG1440.11723138544702608No Hit
NNNTNNTCGGGTATNTNNTNNCATTTGNTCNCCNAGNTTTCGTCTCTCAG1430.11641727860364395No Hit
GANTNNCGGGGTATNTAATNNCATTTGNTCNCCNNGCTTTCGTCTCNCAG1400.11397495807349757No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1390.11316085123011545No Hit
NANTNNCCGGGTNTNTNNTCNCATNTGNTCNCCNAGCNTTCGTCTCTCAG1390.11316085123011545No Hit
GNNTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1380.11234674438673332No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1340.1090903170132048No Hit
GANTANCGGGGTNTCTNNTNNCATTTGCTCCCCTAGNTTTCGTCTCTCAG1340.1090903170132048No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1330.10827621016982268No Hit
GACTACTGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1330.10827621016982268No Hit
NNNTNNTAGGGTATNTNNTNNCATTTGNTCNCCNAGNTTTCGTCTCTCAG1290.10501978279629418No Hit
GACTACTCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1290.10501978279629418No Hit
GANTANCAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1280.10420567595291205No Hit
NNNTNNCGGGGTATNTNNTNNCATTTGNTCNCCNAGNTTTCGTCTCTCAG1270.10339156910952993No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1270.10339156910952993No Hit
GNNTANTAGGGTNTNTNNTNNCATTTGNTCNCNNAGNTTTCGTCTCTNNG1270.10339156910952993No Hit
GNNTANCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1260.1025774622661478No Hit
GANTANCGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1260.1025774622661478No Hit
NANTANTCGGGTATNTANTNNCATTTGNTCNCCNNGNTTTCGTCTCTCAG1250.10176335542276568No Hit
NNNTNNTAGGNNNTNTNATNNCATTTGNTCNCCNAGNTTTCGTCTCNNNG1250.10176335542276568No Hit
GNNTANTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1250.10176335542276568No Hit
GANTACTAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1240.10094924857938356No Hit
NANTANTCGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG1240.10094924857938356No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1240.10094924857938356No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)