FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008120324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129173
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG13221.0234336897029566No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG12450.9638237092890929No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11920.9227934630301998No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11880.9196968406710381No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC11780.9119552847731338No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG11530.8926013950283729No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG11260.8716991941040311No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC10250.793509479535197No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG10130.7842196124577117No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG9940.7695106562516935No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG9460.7323511879417526No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG9320.7215130096846865No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9290.7191905429153151No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG9120.7060298978888777No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8840.6843535413747455No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG8760.6781602966564221No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8650.6696445851687273No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG8600.665773807219775No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8450.6541614733729184No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG7820.605389671216121No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG7640.5914548705998932No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7620.5899065594203122No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG7620.5899065594203122No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7480.5790683811632462No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7410.5736492920347132No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG7110.550424624341No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6970.5395864460839339No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG6960.5388122904941436No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG6830.5287482678268679No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG6310.4884921771577652No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6200.4799764656700704No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG6070.4699124430027947No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6050.46836413182321385No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG5850.4528810200274051No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG5760.4459136197192912No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5750.4451394641295008No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG5560.4304305079234825No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5420.41959232966641635No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5200.40256090669102673No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5170.4002384399216554No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5170.4002384399216554No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5170.4002384399216554No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5130.3971418175624937No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG5040.3901744172543798No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4810.37236883868919973No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG4670.36153066043213367No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG4330.33520937037925885No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG4220.32669365889156404No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG3930.30424314678764136No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG3680.2848892570428805No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG3660.2833409458632996No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3640.28179263468371873No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3180.24618147755335867No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG2900.22450512103922646No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2750.21289278719236993No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2690.2082478536536273No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2470.1912164306782377No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG2450.18966811949865683No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG2430.188119808319076No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2340.18115240801096205No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1740.1347030726235359No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1690.13083229467458368No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1570.12154242759709846No Hit
GACTACTCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1550.1199941164175176No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1350.10451100462170887No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTNTTCCTCCACGCGGCGTCGC1310.10141438226254713No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCNCCACGCTTTCGAGCCTCAG1300.10064022667275668No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)