FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008120326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208428
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG65153.1257796457289806No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG58932.8273552497745027No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG47962.3010344099641125No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG47522.279924002533249No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG47252.2669698888824916No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG45442.180129349223713No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG40141.9258448960792218No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG38251.8351661005239222No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG36391.7459266509298175No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG36101.73201297330493No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG34441.6523691634521271No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG34241.6427735237108259No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG31301.501717619513693No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG31061.490202851824131No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG29311.4062410040877427No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG27101.3002091849463604No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG27041.29733049302397No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG26791.2853359433473428No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG25951.2450342564338766No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG24021.1524363329303164No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG23191.1126144280039152No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG20871.0013050070048168No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG19460.933655746828641No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG18870.9053486095918014No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG18820.9029496996564761No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG16340.7839637668643368No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG16330.7834839848772719No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG15370.7374249141190243No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG14590.7000019191279483No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG13820.6630587061239374No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG12990.6232368011975359No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG12530.6011668297925422No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG12360.593010536012436No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11500.5517492851248392No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG11170.5359164795516916No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG10900.5229623659009346No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG10580.5076093423148522No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG9600.4605907075824745No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG9140.43852073617748094No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8930.4284453144491144No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG8660.4154912007983572No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8590.41213272688890173No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG8580.41165294490183657No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG8420.40397643310879533No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG8240.3953403573416239No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG8150.3910223194580383No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8000.3838255896520621No Hit
GANTANACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG7700.3694321300401098No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG7490.35935670831174316No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7360.35311954247989713No Hit
GANTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7110.3411249928032702No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7000.3358473909455543No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG7000.3358473909455543No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6750.3238528412689274No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6510.31233807357936555No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG6090.29218723012263226No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5840.2801926804460053No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5760.2763544245494847No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5750.2758746425624196No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5740.27539486057535456No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5740.27539486057535456No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5200.24948663327384038No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG4890.23461339167482298No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4880.23413360968775787No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4670.22405818795939125No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4670.22405818795939125No Hit
GACTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4570.21926036808874047No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4480.2149423302051548No Hit
GACTANTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4440.21302320225689447No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG4430.21254342026982936No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4420.2120636382827643No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4280.20534669046385323No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG4070.1952712687354866No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3770.18087780912353427No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3710.1779991172011438No Hit
GANTANACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3670.17607998925288348No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG3560.17080238739516765No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG3510.16840347745984224No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG3500.16792369547277716No Hit
GACTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3480.16696413149864703No Hit
GACTANTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3470.16648434951158192No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3430.16456522156332162No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3370.16168652964093116No Hit
GACTANCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3360.16120674765386608No Hit
GACTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3310.1588078377185407No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3130.1501717619513693No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG2970.14249525015832806No Hit
GACTANCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2970.14249525015832806No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG2950.1415356861841979No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2920.14009634022300266No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG2720.13050070048170112No Hit
GACTANAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2700.12954113650757096No Hit
GACTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2650.12714222657224555No Hit
GACTANCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2640.1266624445851805No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG2640.1266624445851805No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2560.12282418868865988No Hit
GACTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2560.12282418868865988No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2510.12042527875333447No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2420.11610724086974879No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG2410.1156274588826837No Hit
GACTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2410.1156274588826837No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2400.11514767689561863No Hit
GACTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2320.11130942099909802No Hit
GANTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2310.11082963901203294No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2300.11034985702496784No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2240.1074711651025774No Hit
GACTANACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2230.1069913831155123No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG2230.1069913831155123No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2210.10603181914138216No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2200.10555203715431707No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2180.10459247318018693No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG2160.10363290920605676No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG2150.1031531272189917No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2140.10267334523192662No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2130.10219356324486152No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG2110.10123399927073139No Hit
GANTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2090.10027443529660121No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)