FastQCFastQC Report
Wed 8 May 2024
EGAF00008120330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170018
Total Bases51.1 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG52333.0779093978284653No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG46652.7438271241868506No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG46442.7314754908303827No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG41932.466209460174805No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG38522.2656424613864417No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG37122.1832982390099875No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG33851.990965662459269No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG32921.9362655718806243No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG32851.9321483607618015No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG29461.7327577080073875No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG29111.712171652413274No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG27711.6298274300368196No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG26731.5721864743733018No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG25581.5045465774212141No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG25571.5039584044042396No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG25181.4810196567422274No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG25151.4792551376913032No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG21741.2786881389029396No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG21221.2481031420202566No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG20911.2298697784940418No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG19141.1257631544895246No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG18761.103412579844487No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG15950.9381359620746038No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG15260.8975520239033514No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG14850.8734369302073898No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG13530.795798091966733No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG13480.7928572268818596No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11600.682280699690621No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG11410.6711054123681022No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11230.6605182980625581No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG11210.6593419520286087No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG9600.5646460962956863No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG9380.5517062899222435No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8660.509357832700067No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8540.502299756496371No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8200.4823018739192321No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7750.4558340881553718No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG7580.44583514686680237No Hit
GANTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7240.4258372642896634No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG7220.42466091825571417No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG6630.38995871025420836No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6570.38642967215236035No Hit
GANTANACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6340.37290169276194285No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6320.3717253467279935No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG6300.3705490006940442No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6100.3587855403545507No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5910.3476102530320319No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5590.32878871648884234No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5590.32878871648884234No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5470.32173064028514625No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5390.3170252561493489No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5250.3087908339117035No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4990.29349833547036197No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4940.2905574703854886No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4400.2587961274688562No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4330.2546789163500335No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4270.2511498782481855No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4240.24938535919726146No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4170.24526814807843875No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4130.24291545601054007No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4020.23644555282381863No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3900.22938747662012257No Hit
GACTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3820.2246820924843252No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3670.21585949722970507No Hit
GACTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3670.21585949722970507No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3620.2129186321448317No Hit
GACTANTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3520.207036901975085No Hit
GANTANACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3420.20115517180533826No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3240.19056805749979416No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3140.18468632733004742No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3070.18056911621122468No Hit
GACTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3030.178216424143326No Hit
GACTANCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2930.17233469397357926No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA2930.17233469397357926No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2830.16645296380383254No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2730.1605712336340858No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2690.15821854156618712No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA2660.15645402251526308No Hit
GACTANTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2630.15468950346433907No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2630.15468950346433907No Hit
GACTANCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2620.1541013304473644No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2610.15351315743038974No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2570.15116046536249103No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2560.15057229234551636No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2510.147631427260643No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2480.14586690820971895No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2460.14469056217576962No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2440.14351421614182028No Hit
GACTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2440.14351421614182028No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2330.13704431295509886No Hit
GACTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2280.1341034478702255No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG2210.12998623675140278No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2170.1276335446835041No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2150.12645719864955474No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA2150.12645719864955474No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2070.12175181451375737No Hit
GACTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2060.12116364149678269No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2060.12116364149678269No Hit
GACTANACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2030.11939912244585867No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA2030.11939912244585867No Hit
GACTANAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2020.118810949428884No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1960.11528191132703597No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1930.11351739227611195No Hit
GANTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1910.1123410462421626No Hit
GACTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1890.11116470020821326No Hit
GACTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1880.11057652719123857No Hit
GACTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1880.11057652719123857No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1870.1099883541742639No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1870.1099883541742639No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1860.10940018115728922No Hit
GACTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1820.10704748908939053No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1780.10469479702149186No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1750.10293027797056782No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1740.10234210495359314No Hit
GACTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1720.1011657589196438No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)