FastQCFastQC Report
Wed 9 Aug 2023
EGAF00008120333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90490
Sequences flagged as poor quality0
Sequence length301
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC41954.6358713670018785No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC32293.5683500939330317No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC30973.4224776218366673No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC28853.1881975908940214No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC25362.8025196154271192No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC24312.6864846944413747No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC22142.4466791910708365No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT20412.255497845065753No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC20352.248867278152282No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC18622.0576859321471987No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC17241.905182893137363No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC16991.8775555309979004No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC15721.7372085313294285No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT15351.6963200353630234No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC14761.631119460713891No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT14721.626699082771577No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT12911.4266769808818656No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC12331.3625815007183115No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC11381.2575975245883524No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11351.2542822411316168No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC10641.175820532655542No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC10471.1570339264007072No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT10171.1238810918333517No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT8300.917228423030169No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC8170.9028621947176484No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC7940.8774450215493425No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC7890.8719195491214499No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7400.8177699193281025No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT7350.81224444690021No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7000.7735661399049618No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC6880.7603050060780197No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT6870.7591999115924412No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC6860.7580948171068626No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC6150.679633108630788No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5690.6287987622941762No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5560.6144325339816554No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC5400.5967510222123992No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC4820.5326555420488451No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC4750.5249198806497956No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC4680.5171842192507459No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC4180.4619294949718201No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4010.44314288871698526No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3950.43651232180351424No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3800.4199359045198365No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3640.4022543927505802No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3620.4000442037794231No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3340.36910155818322465No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3050.33705381810144763No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3020.33373853464471215No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCTTGAC3000.3315283456735551No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2840.31384683390429885No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2790.3083213614764062No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2580.28511437727925737No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2550.28179909382252183No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2360.26080229859653No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCTTGAC2270.25085644822632336No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2140.23649021991380262No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC2100.23206984197148856No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2080.2298596530003315No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCTTGAC2080.2298596530003315No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCTTGAC1820.2011271963752901No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC1730.19118134600508344No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1680.18565587357719085No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1670.18455077909161233No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1620.17902530666371974No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCTTGAC1600.1768151176925627No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1530.1690794562935131No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1420.15692341695214942No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC1410.15581832246657087No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC1230.1359266217261576No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1220.13482152724057908No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1210.13371643275500056No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC1210.13371643275500056No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC1190.1315062437838435No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1190.1315062437838435No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1150.12708586584152945No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1140.12598077135595093No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1090.12045529892805834No Hit
CCTACGGGTAGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1090.12045529892805834No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCTTGAC1070.11824510995690131No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1060.11714001547132281No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1000.11050944855785169No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1000.11050944855785169No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCTTGAC990.10940435407227318No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC960.10608907061553763No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)