FastQCFastQC Report
Wed 8 May 2024
EGAF00008120358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80389
Total Bases24.1 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG14801.841047904564057No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG14461.7987535608105585No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC14381.7888019505156179No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC13821.7191406784510317No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11831.4715943723643783No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11031.3720782694149696No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC10681.3285399743746036No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC10531.3098807050715893No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC10171.2650984587443557No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9711.2078766995484456No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9121.1344835736232568No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9101.1319956710495218No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8671.0785057657142147No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8531.061090447698068No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8471.0536267399768624No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7440.9254997574294991No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7140.8881812188234708No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7090.8819614623891329No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6750.8396671186356341No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6250.777469554292254No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6040.7513465772680342No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5890.73268730796502No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5880.7314433566781525No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5530.6879050616377862No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4900.609536130565127No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4600.5722175919590988No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4300.5348990533530708No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3980.4950926121733073No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3930.48887285573896927No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3560.44284665812486784No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3110.38686885021582557No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3050.3794051424946199No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2930.3644777270522086No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2660.33089104230678323No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2600.32342733458557765No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2440.30352411399569595No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2400.29854830884822553No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2390.29730435756135787No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2350.29232855241388744No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2350.29232855241388744No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2330.28984064984015223No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2190.2724253318240058No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2120.2637176728159325No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1910.23759469579171277No Hit
GACTACTGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1910.23759469579171277No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1900.2363507445048452No Hit
GACTACTCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1850.23013098807050714No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1850.23013098807050714No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1820.22639913420990435No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1780.22142332906243392No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1760.2189354264886987No Hit
GACTACTAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1750.21769147520183108No Hit
GACTACTCGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1670.20773986490689025No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1640.2040080110462874No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1620.2015201084725522No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1590.1977882546119494No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1580.19654430332508177No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1470.18286083916953813No Hit
GACTACCGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1460.18161688788267052No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1450.18037293659580292No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1400.17415318016146486No Hit
GACTACCCGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1400.17415318016146486No Hit
GACTACCCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1380.17166527758772965No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1330.16544552115339162No Hit
GACTACTGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1330.16544552115339162No Hit
GACTACCAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1300.16171366729278883No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1290.16046971600592122No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1290.16046971600592122No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1280.15922576471905361No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1260.1567378621453184No Hit
GACTACTAGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1240.1542499595715832No Hit
GACTACCGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1230.15300600828471558No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1230.15300600828471558No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1210.15051810571098037No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1200.14927415442411276No Hit
GACTACACGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1140.1418104467029071No Hit
GACTACACGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1120.1393225441291719No Hit
GACTACAAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1100.13683464155543668No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1090.13559069026856907No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1080.13434673898170146No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1070.13310278769483386No Hit
GACTACAGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1060.13185883640796625No Hit
GACTACCAGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1030.12812698254736343No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1030.12812698254736343No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1030.12812698254736343No Hit
GACTACCAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1020.12688303126049583No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1010.12563907997362825No Hit
GACTACTCGGGTATCTAATCCGAAGACCTCCTTCCTCCACGCGGCGTCGC1000.12439512868676064No Hit
GACTACAGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1000.12439512868676064No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC980.12190722611302542No Hit
GACTACCGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA970.12066327482615781No Hit
GACTACAAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC940.11693142096555499No Hit
GACTACACGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA940.11693142096555499No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCCTCAG870.10822376195748175No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA860.10697981067061414No Hit
GACTACTGGGGTATCTAATCCGAAGACCTCCTTCCTCCACGCGGCGTCGC850.10573585938374654No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC840.10449190809687893No Hit
GACTACTCGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA830.10324795681001131No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG820.1020040055231437No Hit
GACTACAGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA810.1007600542362761No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)