FastQCFastQC Report
Wed 8 May 2024
EGAF00008120363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48016
Total Bases14.4 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT12712.6470343218927024No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT10402.165944685104965No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT9111.8972842385871376No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT8631.7973175608130623No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT8091.6848550483172278No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT6731.4016161279573476No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT6631.3807897367544153No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT5441.13295568143952No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5061.0538153948683773No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT4370.910113295568144No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT4340.9038653782072642No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT4190.8726257914028658No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3970.8268077307564144No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3750.7809896701099633No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3410.7101799400199934No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3120.6497834055314895No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2840.5914695101632789No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2400.4998333888703766No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2350.4894201932689104No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2300.4790069976674442No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2290.47692435854715093No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2230.46442852382539157No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2040.42485838053982006No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT2010.41861046317894035No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1980.4123625458180607No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1950.40611462845718094No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1940.40403198933688766No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1820.3790403198933689No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1790.37279240253248913No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1670.34780073308897036No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1660.3457180939686771No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1620.33738753748750416No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1560.32489170276574475No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1540.3207264245251583No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1530.31864378540486504No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1470.30614795068310563No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT1360.28323892035988No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1360.28323892035988No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1360.28323892035988No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT1350.2811562812395868No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1340.2790736421192936No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1340.2790736421192936No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1330.27699100299900037No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1290.2686604465178274No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGACGTGTACATAGCGGAT1290.2686604465178274No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1230.256164611796068No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACTGCGGGAT1230.256164611796068No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1220.25408197267577476No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTGTTAATTGGAT1160.24158613795401532No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT1090.2270076641119627No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT1080.22492502499166944No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT1030.21451182939020325No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCTTCACGGGAT1020.21242919026991003No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT1000.20826391202932357No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT970.20201599466844383No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTGTTAATTGGAT970.20201599466844383No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCTTCACGGGAT950.19785071642785737No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT940.19576807730756415No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT930.1936854381872709No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT900.1874375208263912No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT890.18535488170609796No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT880.18327224258580474No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT880.18327224258580474No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTGTTAATTGGAT870.1811896034655115No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC850.17702432522492503No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCTTCACGGGAT850.17702432522492503No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT820.17077640786404533No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCGGATGTGTAGACTTGGGAT820.17077640786404533No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT820.17077640786404533No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACTGCGGGAT810.16869376874375208No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCTTCACGGGAT810.16869376874375208No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT800.16661112962345886No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT800.16661112962345886No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTGTTAATTGGAT790.16452849050316562No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGTGTCAGATGTGTAACTGCGGGAT790.16452849050316562No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACTGCGGGAT790.16452849050316562No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTGTTAATTGGAT780.16244585138287237No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCTTCACGGGAT780.16244585138287237No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT770.16036321226257916No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTATGATATCAGGAT750.15619793402199267No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTGTTAATTGGAT750.15619793402199267No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGACGTGTACATAGCGGAT740.15411529490169945No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT740.15411529490169945No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGACGTGTACATAGCGGAT720.14995001666111296No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT720.14995001666111296No Hit
CCTACGGGTGGCTGCAGACGTCGGCAGCGTCAGATGTATGATATCGGGAT720.14995001666111296No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGTGTCAGATGTGTAACTGCGGGAT670.1395368210596468No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT660.13745418193935355No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGACGTGTACATAGCGGAT660.13745418193935355No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG660.13745418193935355No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT650.1353715428190603No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT650.1353715428190603No Hit
CCTACGGGTGGCAGCAGACGTCGGCAGCGTCAGATGTATGATATCGGGAT640.13328890369876709No Hit
CCTACGGGTGGCTGCAGTCGTCGGCGGCGTCAGATGTGTTATAACCGGAT640.13328890369876709No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTATGATATCAGGAT630.13120626457847384No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTATGATATCAGGAT630.13120626457847384No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGTTGTGTACATAGAGGAT620.1291236254581806No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGACGTGTCTTCACGGGAT610.12704098633788738No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT610.12704098633788738No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTAGGAT610.12704098633788738No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG600.12495834721759415No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGACGTGTACATAGCGGAT590.12287570809730089No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTAGGAT590.12287570809730089No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG580.12079306897700766No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT570.11871042985671443No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGACGTGTACATAGCGGAT570.11871042985671443No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGACGTGTACATAGCGGAT570.11871042985671443No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCGGATGTGTAGACTTGGGAT570.11871042985671443No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT570.11871042985671443No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCTTCACGGGAT570.11871042985671443No Hit
CCTACGGGGGGCTGCAGTCGTCGGCGGCGTCAGATGTGTTATAACCGGAT560.11662779073642118No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT560.11662779073642118No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTAGGAT560.11662779073642118No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCTTCACGGGAT560.11662779073642118No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGTGTCAGATGTGTAACTGCGGGAT550.11454515161612795No Hit
CCTACGGGTGGCTGCAGTCGTCGGCGGCGTCAGATGTGTACATAGCGGAT540.11246251249583472No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT530.11037987337554149No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT530.11037987337554149No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGTTGTGTACATAGAGGAT520.10829723425524825No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTATGATATCAGGAT520.10829723425524825No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCGGATGTGTAGACTTGGGAT520.10829723425524825No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT520.10829723425524825No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCGGATGTGTAGACTTGGGAT520.10829723425524825No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGTGTCAGATGTGTAACTGCGGGAT500.10413195601466178No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTAGGAT500.10413195601466178No Hit
CCTACGGGTGGCAGCAGTCGTCGGCGGCGTCAGATGTGTTATAACCGGAT490.10204931689436854No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT490.10204931689436854No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT490.10204931689436854No Hit
CCTACGGGCGGCTGCAGACGTCGGCAGCGTCAGATGTATGATATCGGGAT490.10204931689436854No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)