FastQCFastQC Report
Tue 8 Aug 2023
EGAF00008120364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48016
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11262.345051649450183No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9942.070143285571476No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7981.661946017994002No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7811.626541152949017No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7191.4974175274908363No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA6651.3849550149950016No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6361.3245584805064978No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5891.2266744418527158No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA5601.166277907364212No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5281.0996334555148284No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA4881.016327890703099No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4540.9455181606131289No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA4510.9392702432522493No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA4160.866377874041986No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3880.8080639786737753No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3830.7976507830723092No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3560.7414195268243918No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3250.6768577140953015No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3240.6747750749750083No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3170.6601966011329558No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2900.6039653448850383No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2850.5935521492835721No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2790.5810563145618127No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2370.49358547150949683No Hit
GACTACTCGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA2280.4748417194268577No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2210.4602632455848051No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2170.4519326891036321No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2060.4290236587804065No Hit
GACTACTGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1910.397784071976008No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1870.38945351549483503No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1780.3707097634121959No Hit
GACTACCAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1730.3602965678107297No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1630.3394701766077974No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1540.3207264245251583No Hit
GACTACTAGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1530.31864378540486504No Hit
GACTACCAGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1450.30198267244251914No Hit
GACTACCCGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1420.29573475508163943No Hit
GACTACCGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1390.2894868377207597No Hit
GACTACCGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1320.2749083638787071No Hit
GACTACTGGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA1310.2728257247584138No Hit
GACTACTCGGGTATCTAATCCAATTAACACACATCTGACGCTGCCGACGA1300.2707430856381206No Hit
GACTACTCGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA1260.2624125291569477No Hit
GACTACTAGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA1240.2582472509163612No Hit
GACTACAAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1180.24575141619460178No Hit
GACTACTCGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA1170.24366877707430856No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA1130.23533822059313564No Hit
GACTACAGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1100.2290903032322559No Hit
GACTACTCGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA1100.2290903032322559No Hit
GACTACACGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1090.2270076641119627No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1080.22492502499166944No Hit
GACTACTGGGGTATCTAATCCAATTAACACACATCTGACGCTGCCGACGA1080.22492502499166944No Hit
GACTACACGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1070.22284238587137623No Hit
GACTACTCGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA1050.2186771076307897No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1020.21242919026991003No Hit
GACTACCAGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA1020.21242919026991003No Hit
GACTACTCGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA1000.20826391202932357No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG980.20409863378873708No Hit
GACTACAGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA980.20409863378873708No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG970.20201599466844383No Hit
GACTACAAGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA940.19576807730756415No Hit
GACTACTGGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA890.18535488170609796No Hit
GACTACCGGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA890.18535488170609796No Hit
GACTACTGGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA880.18327224258580474No Hit
GACTACTAGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA880.18327224258580474No Hit
GACTACTGGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA870.1811896034655115No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA860.17910696434521825No Hit
GACTACCCGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA860.17910696434521825No Hit
GACTACTAGGGTATCTAATCCAATTAACACACATCTGACGCTGCCGACGA850.17702432522492503No Hit
GACTACCCGGGTATCTAATCCAATTAACACACATCTGACGCTGCCGACGA840.1749416861046318No Hit
GACTACTAGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA830.17285904698433854No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCCGACGCTGCCGACGA820.17077640786404533No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCCGACGCTGCCGACGA820.17077640786404533No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA800.16661112962345886No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG800.16661112962345886No Hit
GACTACTAGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA790.16452849050316562No Hit
GACTACCGGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA790.16452849050316562No Hit
GACTACCAGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA790.16452849050316562No Hit
GACTACTCGGGTATCTAATCCTGATATCATACATCTGACGCTGCCGACGA780.16244585138287237No Hit
GACTACCAGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA760.15828057314228589No Hit
GACTACCCGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA760.15828057314228589No Hit
GACTACCAGGGTATCTAATCCAATTAACACACATCTGACGCTGCCGACGA760.15828057314228589No Hit
GACTACCCGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA750.15619793402199267No Hit
GACTACTGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA740.15411529490169945No Hit
GACTACTCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC740.15411529490169945No Hit
GACTACCGGGGTATCTAATCCAATTAACACACATCTGACGCTGCCGACGA730.15203265578140618No Hit
GACTACCAGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA730.15203265578140618No Hit
GACTACTAGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA720.14995001666111296No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA720.14995001666111296No Hit
GACTACTGGGGTATCTAATCCTGATATCATACATCTGACGCTGCCGACGA720.14995001666111296No Hit
GACTACCGGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA700.1457847384205265No Hit
GACTACTGGGGTATCTAATCCTAAGTCTACACATCTGACGCTGCCGACGA680.14161946017994No Hit
GACTACAAGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA680.14161946017994No Hit
GACTACTCGGGTATCTAATCCTAAGTCTACACATCTGACGCTGCCGACGA670.1395368210596468No Hit
GACTACAGGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA660.13745418193935355No Hit
GACTACCCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC660.13745418193935355No Hit
GACTACTCGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA660.13745418193935355No Hit
GACTACAGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA660.13745418193935355No Hit
GACTACCCGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA660.13745418193935355No Hit
GACTACACGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA640.13328890369876709No Hit
GACTACTCGGGTATCTAATCCGGTTATAACACATCTGACGCCGCCGACGA640.13328890369876709No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA630.13120626457847384No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG630.13120626457847384No Hit
GACTACACGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA620.1291236254581806No Hit
GACTACAGGGGTATCTAATCCAATTAACACACATCTGACGCTGCCGACGA620.1291236254581806No Hit
GACTACCGGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA610.12704098633788738No Hit
GACTACAGGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA600.12495834721759415No Hit
GACTACCGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA590.12287570809730089No Hit
GACTACTAGGGTATCTAATCCTAAGTCTACACATCTGACGCTGCCGACGA590.12287570809730089No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG590.12287570809730089No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCCGACGCTGCCGACGA580.12079306897700766No Hit
GACTACTCGGGTATCTAATCCAGTCCAAACACATCTGACGCTGCCGACGA580.12079306897700766No Hit
GACTACTAGGGTATCTAATCCTGATATCATACATCTGACGCTGCCGACGA570.11871042985671443No Hit
GACTACCCGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA570.11871042985671443No Hit
GACTACCCGGGTATCTAATCCTGATATCATACATCTGACGCTGCCGACGA570.11871042985671443No Hit
GACTACTGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC570.11871042985671443No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG560.11662779073642118No Hit
GACTACTAGGGTATCTAATCCAGTCCAAACACATCTGACGCTGCCGACGA560.11662779073642118No Hit
GACTACTCGGGTATCTAATCCCGATATCATACATCTGACGCTGCCGACGT560.11662779073642118No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG550.11454515161612795No Hit
GACTACTGGGGTATCTAATCCTCTATGTACACAACTGACGCTGCCGACGA540.11246251249583472No Hit
GACTACTCGGGTATCTAATCCTCTATGTACACAACTGACGCTGCCGACGA540.11246251249583472No Hit
GACTACTAGGGTATCTAATCCCGATATCATACATCTGACGCTGCCGACGT540.11246251249583472No Hit
GACTACCAGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA530.11037987337554149No Hit
GACTACAAGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA530.11037987337554149No Hit
GACTACTAGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA530.11037987337554149No Hit
GACTACACGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA520.10829723425524825No Hit
GACTACACGGGTATCTAATCCAATTAACACACATCTGACGCTGCCGACGA520.10829723425524825No Hit
GACTACCAGGGTATCTAATCCCGATATCATACATCTGACGCTGCCGACGT510.10621459513495501No Hit
GACTACAAGGGTATCTAATCCCGATATCATACATCTGACGCTGCCGACGT510.10621459513495501No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCCGCCGACGA510.10621459513495501No Hit
GACTACCCGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA510.10621459513495501No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA500.10413195601466178No Hit
GACTACCGGGGTATCTAATCCCAAGTCTACACATCCGACGCTGCCGACGA500.10413195601466178No Hit
GACTACAAGGGTATCTAATCCAATTAACACACATCTGACGCTGCCGACGA500.10413195601466178No Hit
GACTACTGGGGTATCTAATCCCGATATCATACATCTGACGCTGCCGACGT490.10204931689436854No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA490.10204931689436854No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)