FastQCFastQC Report
Wed 8 May 2024
EGAF00008120372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56343
Total Bases16.9 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG18083.208916813091245No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG16382.9071934401789044No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG13542.4031379230782886No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG12952.2984221642440055No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11642.0659176827644963No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10261.8209892976944784No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9781.7357968159309942No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8171.450047033349307No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7901.402126262357347No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA6041.072005395523845No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA5360.9513160463589089No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA4510.8004543599027386No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA4440.7880304563122306No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4390.7791562394618675No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3840.681539854107875No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3720.6602417336670039No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3610.6407184565962054No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3570.6336190831159151No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3570.6336190831159151No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3290.5839234687538825No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3000.5324530110217773No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2910.5164794206911241No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2900.5147045773210515No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2800.4969561436203255No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2780.49340645688018037No Hit
GACTACTCGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA2570.4561347461086559No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2540.4508102159984382No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2330.4135385052269137No Hit
GACTACTGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA2270.4028894450064782No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2260.4011146016364056No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2150.38159132456560707No Hit
GACTACCAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2040.3620680474948086No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1960.3478693005342278No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1960.3478693005342278No Hit
GACTACCGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1940.34431961379408266No Hit
GACTACTAGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1920.3407699270539375No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1830.3247963367232842No Hit
GACTACCCGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1820.32302149335321156No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1680.2981736861721953No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1650.29284915606197753No Hit
GACTACAAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1490.26445166214081606No Hit
GACTACCGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1460.25912713203059834No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1430.2538026019203805No Hit
GACTACCAGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1380.24492838507001757No Hit
GACTACAGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1360.24137869832987238No Hit
GACTACTCGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA1340.23782901158972722No Hit
GACTACAGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1330.2360541682196546No Hit
GACTACACGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1270.2254051079992191No Hit
GACTACACGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1250.2218554212590739No Hit
GACTACTGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA1220.21653089114885612No Hit
GACTACTGGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA1160.20588183092842055No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1160.20588183092842055No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1110.19700761407805764No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1110.19700761407805764No Hit
GACTACAAGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1090.19345792733791242No Hit
GACTACTCGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA1050.18635855385762207No Hit
GACTACTGGGGTATCTAATCCAATTAACACACATCTGACGCTGCCGACGA1050.18635855385762207No Hit
GACTACTCGGGTATCTAATCCAATTAACACACATCTGACGCTGCCGACGA1010.1792591803773317No Hit
GACTACTGGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA1000.17748433700725913No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG990.17570949363718652No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG970.17215980689704133No Hit
GACTACTCGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA970.17215980689704133No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA960.17038496352696875No Hit
GACTACTGGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA950.16861012015689616No Hit
GACTACCCGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA930.16506043341675097No Hit
GACTACTAGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA930.16506043341675097No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA900.1597359033065332No Hit
GACTACTCGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA890.15796105993646062No Hit
GACTACCCGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA880.156186216566388No Hit
GACTACTCGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA860.15263652982624284No Hit
GACTACTAGGGTATCTAATCCAATTAACACACATCTGACGCTGCCGACGA850.15086168645617024No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCCGACGCTGCCGACGA840.14908684308609765No Hit
GACTACCAGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA820.14553715634595246No Hit
GACTACCCGGGTATCTAATCCAATTAACACACATCTGACGCTGCCGACGA820.14553715634595246No Hit
GACTACCAGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA810.14376231297587988No Hit
GACTACCAGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA800.1419874696058073No Hit
GACTACTAGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA800.1419874696058073No Hit
GACTACTAGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA790.14021262623573472No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA790.14021262623573472No Hit
GACTACTAGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA770.1366629394955895No Hit
GACTACTCGGGTATCTAATCCAGTCCAAACACATCTGACGCTGCCGACGA770.1366629394955895No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCCGACGCTGCCGACGA760.13488809612551692No Hit
GACTACTGGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA760.13488809612551692No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA760.13488809612551692No Hit
GACTACCAGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA740.13133840938537175No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCCGACGCTGCCGACGA730.12956356601529917No Hit
GACTACTCGGGTATCTAATCCTAAGTCTACACATCTGACGCTGCCGACGA730.12956356601529917No Hit
GACTACCGGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA720.12778872264522656No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA710.12601387927515395No Hit
GACTACCAGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA700.12423903590508137No Hit
GACTACTCGGGTATCTAATCCCGATATCATACATCTGACGCTGCCGACGT690.12246419253500879No Hit
GACTACCGGGGTATCTAATCCAATTAACACACATCTGACGCTGCCGACGA690.12246419253500879No Hit
GACTACTGGGGTATCTAATCCCGTGAAGACACGTCTGACGCTGCCGACGA680.12068934916493619No Hit
GACTACTCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC670.11891450579486361No Hit
GACTACTGGGGTATCTAATCCCGATATCATACATCTGACGCTGCCGACGT670.11891450579486361No Hit
GACTACTCGGGTATCTAATCCCGTGAAGACACGTCTGACGCTGCCGACGA660.11713966242479101No Hit
GACTACCCGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA660.11713966242479101No Hit
GACTACTAGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA660.11713966242479101No Hit
GACTACCGGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA650.1153648190547184No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA640.11358997568464582No Hit
GACTACTCGGGTATCTAATCCGGTTATAACACATCTGACGCCGCCGACGA640.11358997568464582No Hit
GACTACCCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC630.11181513231457324No Hit
GACTACCCGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA630.11181513231457324No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC630.11181513231457324No Hit
GACTACAGGGGTATCTAATCCAATTAACACACATCTGACGCTGCCGACGA620.11004028894450064No Hit
GACTACTGGGGTATCTAATCCTAAGTCTACACATCTGACGCTGCCGACGA610.10826544557442806No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA610.10826544557442806No Hit
GACTACAAGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA610.10826544557442806No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACACATCCGACGCTGCCGACGA610.10826544557442806No Hit
GACTACCCGGGTATCTAATCCGGTTATAACACATCTGACGCCGCCGACGA610.10826544557442806No Hit
GACTACCCGGGTATCTAATCCTAAGTCTACACATCTGACGCTGCCGACGA610.10826544557442806No Hit
GACTACTAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC600.10649060220435547No Hit
GACTACTGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC600.10649060220435547No Hit
GACTACTGGGGTATCTAATCCTGATATCATACATCTGACGCTGCCGACGA590.10471575883428288No Hit
GACTACCGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA580.10294091546421028No Hit
GACTACTCGGGTATCTAATCCTGATATCATACATCTGACGCTGCCGACGA580.10294091546421028No Hit
GACTACCGGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA580.10294091546421028No Hit
GACTACAAGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA580.10294091546421028No Hit
GACTACAGGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA570.10116607209413768No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)