FastQCFastQC Report
Wed 9 Aug 2023
EGAF00008120397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63484
Sequences flagged as poor quality0
Sequence length301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT16032.525045680801462No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11951.8823640602356502No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11381.792577657362485No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC11131.753197656102325No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT9751.5358200491462417No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT9631.516917648541365No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT9511.4980152479364879No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC8911.4035032449121039No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC7961.2538592401234956No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT7941.250708840022683No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC7571.192426438157646No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7501.1814000378048013No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT7211.1357192363430155No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC7191.1325688362422026No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC6300.9923760317560331No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT6240.9829248314535947No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC6170.9718984311007497No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6050.9529960304958729No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5840.9199168294373385No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5780.9104656291349001No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC5460.8600592275218952No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC5300.8348560267153928No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC5290.8332808266649864No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4760.7497952239934472No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4260.6710352214731271No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC4250.6694600214227207No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4140.6521328208682503No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4060.6395312204649991No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3840.6048768193560582No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3820.6017264192552454No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3760.592275218952807No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3400.5355680171381766No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3370.5308424169869573No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3310.5213912166845189No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3260.5135152164324869No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3030.4772856152731397No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2950.4646840148698885No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2710.42687921366013487No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2660.4190032134081028No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2570.40482641295444516No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2300.36229601159347236No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2290.36072081154306596No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2130.33551761073656355No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2070.32606641043412515No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1880.2961376094764035No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1860.2929872093755907No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1700.2677840085690883No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1550.24415600781299224No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1540.24258080776258586No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1380.2173776069560834No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1380.2173776069560834No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1340.21107680675445784No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1290.2032008065024258No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1240.1953248062503938No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1230.19374960619998738No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1230.19374960619998738No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1200.1890240060487682No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1170.18429840589754898No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1160.18272320584714258No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1120.17642240564551698No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1070.16854640539348498No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1030.16224560519185938No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1020.16067040514145295No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1000.15752000504064015No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT980.15436960493982738No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT980.15436960493982738No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC970.15279440488942095No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT960.15121920483901455No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT950.14964400478860815No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC950.14964400478860815No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC940.14806880473820175No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC930.14649360468779535No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC920.14491840463738895No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC920.14491840463738895No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT910.14334320458698255No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC900.14176800453657615No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT840.13231680423413775No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT830.13074160418373135No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC820.12916640413332495No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT810.12759120408291852No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT810.12759120408291852No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC780.12286560393169933No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT710.1118392035788545No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC690.1086888034780417No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT690.1086888034780417No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC680.10711360342763532No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC670.10553840337722892No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGACGTGTACATAGCGGAT670.10553840337722892No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTGTTAATTGGAT660.10396320332682252No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT650.1023880032764161No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCTTCACGGGAT650.1023880032764161No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGACGTGTACATAGCGGAT640.1008128032260097No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACTGCGGGAT640.1008128032260097No Hit
CCTACGGGTGGCTGCAGACGTCGGCAGCGTCAGATGTATGATATCGGGAT640.1008128032260097No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)