FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008120399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106395
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT33743.171201654213074No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT26172.4597020536679355No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT25592.405188213731848No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT22322.0978429437473562No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT19931.8732083274589972No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT19451.828093425442925No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT17701.6636120118426616No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT16691.56868273885051No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT15371.4446167583063114No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT14611.3731848301141971No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT11961.1241129752337986No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT11891.1175337186897882No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT10761.011325720193618No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT8900.8365054748813385No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8730.8205272804173128No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT7360.6917618309131068No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7030.6607453357770572No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT5600.5263405235208422No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT5460.5131820104328211No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT4910.46148785187273844No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT4520.4248319939846797No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT4030.37877719817660604No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3940.3703181540485925No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3550.3336622961605339No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT3440.32332346444851734No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3380.3176841016965083No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT3260.30640537619249025No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3050.2866676065604587No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3030.28478781897645566No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2900.2725691996804361No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2860.2688096245124301No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2630.24719206729639553No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT2570.24155270454438652No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2490.23403355420837443No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGATTGCTGGACGACAACTGACG2400.22557451008036095No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGCTCTCTGGTCTGTAACTGACG2350.22087504112035342No Hit
CCTACGGGGGGCTGCAGTGGGGAAGGCGGCTCTCTGGTCTGTAACTGACG2340.21993514732835193No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2320.21805535974434886No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2290.2152356783683444No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2290.2152356783683444No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2240.21053620940833687No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2210.20771652803233234No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2120.19925748390431883No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2110.19831759011231734No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT2040.19173833356830677No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1980.18609897081629775No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1850.1738803515202782No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1810.17012077635227218No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGATTGCTGGACAGTAACTGACG1810.17012077635227218No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1760.16542130739226468No Hit
CCTACGGGGGGCTGCAGTGGGGAAGGCGGATTGCTGGACGACAACTGACG1730.16260162601626016No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1670.15696226326425114No Hit
CCTACGGGCGGCTGCAGTGGGGAAGGCGGATTGCTGGACGACAACTGACG1670.15696226326425114No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1640.15414258188824664No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTGTTAATTGGAT1630.15320268809624513No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1620.1522627943042436No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1620.1522627943042436No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1590.1494431129282391No Hit
CCTACGGGCGGCTGCAGTGGGGAAGGCGGCTCTCTGGTCTGTAACTGACG1590.1494431129282391No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1590.1494431129282391No Hit
CCTACGGGGGGCAGCAGTGGGGAAGGCGGATTGCTGGACGACAACTGACG1520.14286385638422858No Hit
CCTACGGGTGGCTGCAGTGGTGAAGGCGGATTGCTGGACAGTAACTGACG1470.13816438742422107No Hit
CCTACGGGTGGCAGCAGTGGGGAAGGCGGATTGCTGGACGACAACTGACG1460.13722449363221956No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1440.13534470604821655No Hit
CCTACGGGTGGCAGCAGTGGTGAAGGCGGATTGCTGGACAGTAACTGACG1430.13440481225621506No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGACGTGTACATAGCGGAT1430.13440481225621506No Hit
CCTACGGGTGGCAGCAGTGGGGAAGGCGGCTCTCTGGTCTGTAACTGACG1420.13346491846421354No Hit
CCTACGGGGGGCTGCAGTGGGGAAGGCGGATTGCTGGACAGTAACTGACG1410.13252502467221206No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1400.13158513088021054No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT1390.13064523708820902No Hit
CCTACGGGGGGCAGCAGTGGGGAAGGCGGCTCTCTGGTCTGTAACTGACG1260.11842661779218949No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACTGCGGGAT1240.11654683020818647No Hit
CCTACGGGTGGCTGCAGACGTCGGCAGCGTCAGATGTATGATATCGGGAT1210.11372714883218196No Hit
CCTACGGGTGGCTGCAGTCGTCGGCGGCGTCAGATGTGTTATAACCGGAT1200.11278725504018047No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1190.11184736124817896No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1170.10996757366417596No Hit
CCTACGGGCGGCAGCAGTGGGGAAGGCGGATTGCTGGACGACAACTGACG1160.10902767987217443No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1160.10902767987217443No Hit
CCTACGGGTGGCAGCAGACGTCGGCAGCGTCAGATGTATGATATCGGGAT1130.10620799849616992No Hit
CCTACGGGCGGCAGCAGTGGGGAAGGCGGCTCTCTGGTCTGTAACTGACG1110.10432821091216693No Hit
CCTACGGGCGGCTGCAGTGGTGAAGGCGGATTGCTGGACAGTAACTGACG1110.10432821091216693No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTGTTAATTGGAT1110.10432821091216693No Hit
CCTACGGGTAGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1100.10338831712016541No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1100.10338831712016541No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)