FastQCFastQC Report
Wed 2 Aug 2023
EGAF00008120410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78889
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG66228.39407268440467No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG60957.726045456274005No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG47486.018583072418208No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG47466.016047864721317No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG43375.497597890707196No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG40115.084359036114034No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG38364.862528362636109No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG30523.868726945455006No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG29433.7305581259744702No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4900.621125885738189No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4870.6173230741928533No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4820.6109850549506268No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4370.553942881770589No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3880.49183029319677013No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3800.48168946240920785No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3770.477886650863872No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3700.469013423924755No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3700.469013423924755No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3440.43605572386517766No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3290.41704166613849836No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3110.39422479686648326No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2820.35746428526157004No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2780.3523938698677889No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2770.3511262660193436No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2750.3485910583224531No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2580.32704179289888324No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2530.32070377365665687No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2500.316900962111321No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2440.30929533902064926No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2330.29535169668775113No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2250.28521086590018885No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2220.281408054354853No Hit
GACTACTGGGGTATCTAATCNTGTTTGNTCCCCACGNTTTCGCACATCAG2120.2687320158704002No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1970.24971795814372091No Hit
GACTACTCGGGTATCTAATCNTGTTTGNTCCCCACGNTTTCGCACATCAG1950.24718275044683038No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1950.24718275044683038No Hit
GACTACTCGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG1910.24211233505304922No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1890.23957712735615866No Hit
GACTACTGGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG1740.2205630696294794No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1720.21802786193258883No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1650.20915463499347184No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1630.20661942729658125No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1630.20661942729658125No Hit
GACTACCGGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG1600.2028166157512454No Hit
GACTACTAGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG1590.20154901190280014No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1590.20154901190280014No Hit
GACTACTAGGGTATCTAATCNTGTTTGNTCCCCACGNTTTCGCACATCAG1560.1977462003574643No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1500.1901405772667926No Hit
GACTACCGGGGTATCTAATCNTGTTTGNTCCCCACGNTTTCGCACATCAG1420.1799997464792303No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1410.17873214263078502No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1380.17492933108544917No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1360.1723941233885586No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1330.16859131184322276No Hit
GACTACCCGGGTATCTAATCNTGTTTGNTCCCCACGNTTTCGCACATCAG1300.16478850029788691No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1270.16098568875255106No Hit
GACTACCCGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG1250.1584504810556605No Hit
GACTACCAGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG1230.15591527335876992No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1180.1495772541165435No Hit
GACTACCAGGGTATCTAATCNTGTTTGNTCCCCACGNTTTCGCACATCAG1160.14704204641965293No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1150.14577444257120764No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1140.14450683872276235No Hit
GACTACAGGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG1140.14450683872276235No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1140.14450683872276235No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG1090.13816881948053594No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1070.13563361178364539No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1060.1343660079352001No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1030.13056319638986424No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG1030.13056319638986424No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1020.12929559254141895No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG990.1254927809960831No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG990.1254927809960831No Hit
GACTACACGGGTATCTAATCNTGTTTGNTCCCCACGNTTTCGCACATCAG960.12168996945074725No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG940.11915476175385668No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG940.11915476175385668No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG930.1178871579054114No Hit
GACTACAAGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG920.11661955405696611No Hit
GACTACAGGGGTATCTAATCNTGTTTGNTCCCCACGNTTTCGCACATCAG910.11535195020852082No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG910.11535195020852082No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG900.11408434636007556No Hit
GACTACACGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG850.10774632711784912No Hit
GACTACAAGGGTATCTAATCNTGTTTGNTCCCCACGNTTTCGCACATCAG800.1014083078756227No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)