FastQCFastQC Report
Tue 8 Aug 2023
EGAF00008120412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52835
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG534910.123970852654491No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG47579.003501466830699No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG38637.311441279454907No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG38397.266016844894482No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG33396.319674458218984No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG32266.105801078830321No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG29615.6042396138923065No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG24364.610580107883032No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG22804.3153212832402765No Hit
GACTACTGGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG1640.3104003028295637No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGNTTTCGCACATCAG1620.3066149332828617No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGNTTTCGCACATCAG1500.28390271600264977No Hit
GACTACTAGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG1390.26308318349578874No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1380.26119049872243777No Hit
GACTACTCGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG1370.2592978139490868No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGNTTTCGCACATCAG1340.25361975962903377No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGNTTTCGCACATCAG1220.2309075423488218No Hit
GACTACCCGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG1180.2233368032554178No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1170.2214441184820668No Hit
GACTACCGGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG1160.2195514337087158No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1150.2176587489353648No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGNTTTCGCACATCAG1130.21387337938866283No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1050.19873190120185485No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGNTTTCGCACATCAG1050.19873190120185485No Hit
GACTACCAGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG1030.19494653165515283No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG1020.19305384688180183No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1010.19116116210845083No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG990.18737579256174886No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGNTTTCGCACATCAG910.17223431437494086No Hit
GACTACACGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG910.17223431437494086No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG880.16655626005488786No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGNTTTCGCACATCAG880.16655626005488786No Hit
GACTACAGGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG870.16466357528153686No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG810.15330746664143086No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG810.15330746664143086No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG770.14573672754802688No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGNTTTCGCACATCAG750.14195135800132488No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG710.13438061890792088No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG680.12870256458786788No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG680.12870256458786788No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG650.1230245102678149No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG640.1211318254944639No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG630.11923914072111291No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG630.11923914072111291No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG630.11923914072111291No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG630.11923914072111291No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG630.11923914072111291No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG610.1154537711744109No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG610.1154537711744109No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG600.1135610864010599No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG580.1097757168543579No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG570.10788303208100691No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG570.10788303208100691No Hit
GACTACAAGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG560.10599034730765591No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG560.10599034730765591No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG550.10409766253430491No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG540.10220497776095391No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG540.10220497776095391No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)