FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008120420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159006
Sequences flagged as poor quality0
Sequence length301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG130978.236796095744815No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG123627.774549388073406No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG97166.110461240456335No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG96516.069582279913965No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG82755.204206130586267No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG75344.738185980403255No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG73024.59227953662126No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG62793.9489075883928906No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG53883.3885513754197953No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG30361.9093619108712878No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG27591.7351546482522673No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG22171.3942870080374326No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG21591.3578103970919335No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG17681.1119077267524495No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG16691.0496459253109947No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG16201.0188294781329008No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG14580.9169465303196105No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG12380.7785869715608216No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4760.2993597725871979No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4590.2886683521376552No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGNTTTCGCTCCTCAG3740.23521124988994127No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3650.22955108612253625No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3480.21885966567299348No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGNTTTCGCTCCTCAG3410.21445731607612292No Hit
GACTACTCGGGTATCTAATCNTGTTCGCTCCCCACGCTTTCGCTCCTCAG3390.21319950190558848No Hit
GACTACTGGGGTATCTAATCNTGTTCGCTCCCCACGCTTTCGCTCCTCAG3340.21005496647925237No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3040.1911877539212357No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2970.18678540432436513No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2900.18238305472749455No Hit
GACTACCGGGGTATCTAATCNTGTTCGCTCCCCACGCTTTCGCTCCTCAG2820.17735179804535678No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2820.17735179804535678No Hit
GACTACTAGGGTATCTAATCNTGTTCGCTCCCCACGCTTTCGCTCCTCAG2790.1754650767895551No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGNTTTCGCTCCTCAG2600.16351584216947787No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2550.16037130674314176No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2550.16037130674314176No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2480.1559689571462712No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGNTTTCGCTCCTCAG2460.15471114297573677No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGNTTTCGCTCCTCAG2450.15408223589046954No Hit
GACTACCCGGGTATCTAATCNTGTTCGCTCCCCACGCTTTCGCTCCTCAG2410.15156660754940066No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2410.15156660754940066No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2380.14967988629359896No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2360.14842207212306455No Hit
GACTACAGGGGTATCTAATCNTGTTCGCTCCCCACGCTTTCGCTCCTCAG2350.1477931650377973No Hit
GACTACCAGGGTATCTAATCNTGTTCGCTCCCCACGCTTTCGCTCCTCAG2310.1452775366967284No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2270.14276190835565955No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCTCCTCAG2240.14087518709985786No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2210.1389884658440562No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2140.13458611624718564No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCTCCTCAG2110.13269939499138397No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2090.13144158082084953No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGNTTTCGCTCCTCAG2080.1308126737355823No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGCACATCAG2060.12955485956504786No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2060.12955485956504786No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2040.12829704539451342No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGNTTTCGCTCCTCAG1960.12326578871237562No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1890.11886343911550507No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1860.1169767178597034No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1800.11320327534810007No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1780.11194546117756564No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1770.1113165540922984No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1770.1113165540922984No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCTCCTCAG1760.11068764700703118No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGCACATCAG1760.11068764700703118No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1750.11005873992176395No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1730.10880092575122952No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1690.10628529741016063No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1620.10188294781329006No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)