FastQCFastQC Report
Wed 8 May 2024
EGAF00008120436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179834
Total Bases54.1 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG111556.2029427138360935No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG102885.720831433433055No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG83064.618703915833491No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG79434.416851096010765No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG66803.714536739437481No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG64533.5883092185015073No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG64223.571071098902321No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG55793.1023054594792976No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG48202.6802495634863264No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG32841.8261285407653725No Hit
GANTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG30441.6926721309652233No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG29381.633728883303491No Hit
GANTANTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG26181.455787003569959No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG25241.403516576398234No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG24551.365147858580691No Hit
GANTANCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG21641.2033319616980103No Hit
GANTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG20951.1649632438804676No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG20911.1627389703837985No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG18701.0398478596928278No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG18611.0348432443253222No Hit
GANTANCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG17800.989801706017772No Hit
GANTANAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG16510.9180688857501917No Hit
GANTANCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG16320.9075035866410133No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG16170.8991625610285041No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG14350.7979581169300577No Hit
GANTANACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG14110.7846124759500429No Hit
GANTANAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG12450.6923051258382731No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10840.6027781175973398No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9920.5516198271739493No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG9360.5204799982205812No Hit
GACTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG8960.4982372632538897No Hit
GACTANTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG8160.45375179332050664No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7890.43873794721799No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7450.4142709387546292No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7090.39425247728460694No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6710.37312187906625No Hit
GACTANCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6310.3508791440995585No Hit
GACTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6160.3425381184870492No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG6030.33530922962287446No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5820.3236317937653614No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5820.3236317937653614No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5740.3191832467720231No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5450.30305726392117177No Hit
GACTANCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5250.29193589643782597No Hit
GACTANAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4910.27302957171613823No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4780.2658006828519635No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4760.2646885461036289No Hit
GACTANCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4730.26302034098112703No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4480.24911863162694484No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4440.24689435813027572No Hit
GACTANACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4320.24022153764026827No Hit
GNNTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4050.22520769153775147No Hit
GANTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4040.2246516231635842No Hit
GANTANACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3840.21353025568023845No Hit
GANTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3750.20852564031273285No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3620.20129675144855813No Hit
GANTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3410.18961931559104508No Hit
GACTANAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3380.1879511104685432No Hit
GNNTANTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3370.18739504209437594No Hit
GNNTANCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2890.16070376013434612No Hit
GANTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2800.15569914476684055No Hit
GNNTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2800.15569914476684055No Hit
GACTANTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2520.14012923029015648No Hit
GANTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2500.1390170935418219No Hit
GANTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2490.13846102516765463No Hit
GNNTANCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2450.13623675167098545No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2390.13290034142598176No Hit
GANTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2340.1301199995551453No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG2180.1212229055684687No Hit
GACTANTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2040.11343794833012666No Hit
GNNTANAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2020.1123258115817921No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2020.1123258115817921No Hit
GACTANCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2010.1117697432076248No Hit
GNNTANACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1990.11065760645929024No Hit
GNNTANCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1970.10954546971095565No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1970.10954546971095565No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1960.10898940133678837No Hit
GANTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1950.10843333296262109No Hit
GANTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1930.10732119621428651No Hit
GACTANTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1840.10231658084678091No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG1830.10176051247261363No Hit
GNNTANAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1820.10120444409844635No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)