FastQCFastQC Report
Wed 8 May 2024
EGAF00008120444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74254
Total Bases22.3 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8051.0841166805828644No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7811.0517951894847415No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6230.8390120397554341No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6220.837665310959679No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5520.7433942952568212No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5180.6976055162011474No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5060.6814447706520861No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4860.6545101947369839No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4630.6235354324346163No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4310.5804401109704528No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4300.5790933821746976No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4070.54811861987233No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3850.5184905863657177No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3790.510410213591187No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3700.498289654429391No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3650.4915560104506155No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3560.4794354512888194No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3510.4727018073100439No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3500.47135507851428876No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTTCTCAG3310.4457672313949417No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3300.44442050259918653No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3100.4174859266840843No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3030.4080588251137986No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2990.4026719099307781No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2900.3905513507689821No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2810.3784307916071861No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2660.35822985967085946No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2600.3501494868963288No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTTCTCAG2540.34206911412179813No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2520.3393756565302879No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2470.3326420125515124No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTTCTCAG2430.32725509736849195No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2430.32725509736849195No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2190.2949336062703693No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTTCTCAG2160.29089341988310397No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2150.2895466910873488No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2150.2895466910873488No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2080.28011958951706306No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTTCTCAG2030.2733859455382875No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2010.2706924879467773No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1990.26799903035526706No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTTCTCAG1760.2370242680528995No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTTCTCAG1690.22759716648261372No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1650.22221025129959326No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG1640.22086352250383817No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG1550.20874296334204215No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1540.20739623454628706No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1500.2020093193632666No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1490.20066259056751154No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1470.19796913297600133No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1470.19796913297600133No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1450.19527567538449106No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1420.19123548899722573No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1410.18988876020147064No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTTCTCAG1330.17911492983542973No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1330.17911492983542973No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1290.17372801465240928No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG1270.17103455706089907No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG1240.16699437067363376No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1230.16564764187787864No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1220.16430091308212352No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTTCTCAG1200.1616074554906133No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1180.1589139978991031No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1170.15756726910334798No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG1170.15756726910334798No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1170.15756726910334798No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1150.15487381151183777No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1120.1508336251245724No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1070.14409998114579686No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1060.14275325235004174No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG1040.14005979475853153No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1040.14005979475853153No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1030.1387130659627764No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1030.1387130659627764No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG1010.1360196083712662No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG990.13332615077975596No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG960.12928596439249063No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG950.12793923559673553No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG880.11851213402644975No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG870.11716540523069463No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG870.11716540523069463No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCNCCACGCTTTCGAGCCTCAG870.11716540523069463No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG860.11581867643493952No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG860.11581867643493952No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCNCCACGCTTTCGAGCCTCAG860.11581867643493952No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG860.11581867643493952No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG860.11581867643493952No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG850.11447194763918442No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA850.11447194763918442No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG840.1131252188434293No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA840.1131252188434293No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG830.11177849004767419No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCNCCACGCTTTCGAGCCTCAG810.10908503245616397No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG800.10773830366040886No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG770.10369811727314353No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG770.10369811727314353No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTNTTCCTCCACGCGGCGTCGC770.10369811727314353No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA760.10235138847738841No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG760.10235138847738841No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)