FastQCFastQC Report
Wed 8 May 2024
EGAF00008120450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124062
Total Bases37.3 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG88347.120633231771212No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG80986.527381470555045No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG67865.46984572230014No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG61024.918508487691638No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG54494.392158759330012No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG51744.17049539746256No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG49533.9923586593799874No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG48123.878705808386129No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG45453.6634908352275475No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG39813.208879431252116No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG38693.118601989327917No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG37042.9856039722074446No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG34792.804243039770437No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG32502.6196579129789943No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG29192.3528558301494416No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG28232.275475165642985No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG24711.9917460624526446No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG21251.7128532507939578No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG3760.3030742693169544No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3750.3022682207283455No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3220.25954764553207266No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG3050.245844819525721No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2760.22246941045606228No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG2600.20957263303831958No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2560.20634843868388386No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2520.20312424432944817No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2470.1990940013864036No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2350.1894214183230965No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2290.18458512679144298No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2120.1708823007850913No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2070.16685205784204674No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2030.16362786348761105No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG2020.1628218148990021No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG2020.1628218148990021No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2020.1628218148990021No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG2010.1620157663103932No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG2010.1620157663103932No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1980.15959762054456642No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1910.15395528042430398No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1870.1507310860698683No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG1790.14428269736099691No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1730.1394464058293434No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1680.1354161628862988No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1670.13461011429768988No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1620.13057987135464524No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1590.1281617255888185No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG1580.12735567700020956No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1580.12735567700020956No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1550.12493753123438281No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1420.11445889958246684No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1360.1096226080508133No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1330.10720446228498653No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCACATCAG1320.10639841369637763No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1320.10639841369637763No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1320.10639841369637763No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1290.10398026793055086No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)