FastQCFastQC Report
Wed 8 May 2024
EGAF00008120458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104335
Total Bases31.4 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG29112.790051277136148No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG27632.648200507979106No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG22192.1268030862126803No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG21042.0165812047730864No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC20241.9399051133368477No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC19911.908276225619399No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG19011.82201562275363No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG16471.5785690324435713No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG16001.533521828724781No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC14771.4156323381415632No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC14501.3897541572818326No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC13911.3332055398476061No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG13791.3217041261321705No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC12891.2354435232664016No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG12181.1673934921167393No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC11461.0983850098241241No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC10200.9776201658120477No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8770.8405616523697704No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA5950.5702784300570278No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA5530.5300234820530023No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA4310.41309244261273786No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA4150.39775722432549No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4010.3843389083241482No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3800.36421143432213543No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3750.3594191786073705No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3520.33737480231945177No Hit
GACTACTGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC3340.320122681746298No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3080.2952029520295203No Hit
GACTACTCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC3070.2942445008865673No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3010.2884937940288494No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2940.28178463602817844No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2890.2769923803134135No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2810.2693247711697896No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2750.2635740643120717No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2570.24632194373891791No Hit
GACTACTCGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA2450.23482053002348205No Hit
GACTACTAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC2360.22619446973690516No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2350.22523601859395218No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2300.22044376287918724No Hit
GACTACCGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC2280.21852686059328125No Hit
GACTACTGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA2150.20606699573489243No Hit
GACTACCCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC2120.20319164230603345No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2120.20319164230603345No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2070.19839938659126852No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2060.19744093544831554No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1940.18593952173287967No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1920.18402261944697368No Hit
GACTACTAGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1910.18306416830402072No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1840.17635501030334977No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1820.17443810801744383No Hit
GACTACCAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1760.16868740115972589No Hit
GACTACCGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1750.16772895001677288No Hit
GACTACCAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1730.16581204773086694No Hit
GACTACAGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1720.16485359658791393No Hit
GACTACACGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1640.15718598744429005No Hit
GACTACCCGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1550.14855992715771316No Hit
GACTACACGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1520.14568457372885416No Hit
GACTACAGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1490.1428092202999952No Hit
GACTACAAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1470.14089231801408922No Hit
GACTACCGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1460.13993386687113626No Hit
GACTACCAGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1440.13801696458523027No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1430.13705851344227726No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1430.13705851344227726No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1420.13610006229932428No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1390.13322470887046534No Hit
GACTACTGGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA1340.12843245315570037No Hit
GACTACAGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1310.12555709972684143No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1300.12459864858388844No Hit
GACTACTCGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA1280.12268174629798247No Hit
GACTACAAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1270.12172329515502947No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1260.12076484401207649No Hit
GACTACACGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1250.1198063928691235No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA1200.11501413715435856No Hit
GACTACTGGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA1180.11309723486845258No Hit
GACTACTCGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA1130.10830497915368764No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCCTCAG1130.10830497915368764No Hit
GACTACTCGGGTATCTAATCCAATTAACACACATCTGACGCTGCCGACGA1120.10734652801073466No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)