FastQCFastQC Report
Thu 31 Aug 2023
EGAF00008120688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11900886
Sequences flagged as poor quality0
Sequence length75
%GC63

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCCGCGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGATCTTTCCC781540.6567074081711227No Hit
GTCCGCGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGATCTTTCCCG725700.6097865318598968No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCC584540.4911735143080943No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTTAGTTCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA561080.4714606962876545Illumina Single End PCR Primer 1 (96% over 32bp)
GCAGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCC521810.43846315307952705No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACC507490.4264304355154734No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCGCGGGGAATCCCCCGC494530.4155404900105757No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCG414710.3484698534209974No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGC406290.34139474993710556No Hit
CGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATG348880.29315464411641284No Hit
GCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCGCGGGGAA348550.2928773538373529No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGC347940.2923647869578786No Hit
CCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCGCGGGGAATCCCCCG322120.27066892330537407No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCC321890.27047566038360504No Hit
GGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACCGG319070.2681060889080023No Hit
GGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCGCGGGGAATCCCCCGCGAGGGGGGTC301860.2536449807182423No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT293720.24680515383476492No Hit
GGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGA289520.24327600482854805No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT287430.24151983306116873No Hit
CGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGA275010.23108363528564174No Hit
CCGCGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGATCTTTCCCGCC272850.2292686443681588No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG271690.22829392702358461No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGT255120.21437059392048624No Hit
CGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCGCGGGGA236310.1985650480140722No Hit
CGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCGCGGGGAATCCCCCGCG229970.1932377135618306No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGG227340.19102779406508053No Hit
GGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGG220000.18486018603993012No Hit
CGGCGGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCCGCGACCG209970.17643224210365513No Hit
GTTACAGCCCCCCCGGCAGCAGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCC207040.17397024053503243No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGT200240.1682563802392528No Hit
CCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCGCGGGGAATCCCCCGCGAGGGGGGTCTCCCCCGCGG195370.16416424793918705No Hit
CGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGC193490.16258453362211855No Hit
CGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACCGGCGG183730.15438346355052893No Hit
GGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT180170.1513920896309737No Hit
GTCCGCGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGATCTTTCCCC175170.14719072176642983No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATG174910.14697225063747354No Hit
CAGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCC171040.1437203919103166No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAAT170180.14299775663761505No Hit
CTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCGCGGG169720.142611230794077No Hit
GCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGG169680.14257761985116066No Hit
CTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCG169250.14221630221480988No Hit
CGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGA161970.136099110604034No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCC157360.13222544943292455No Hit
GGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCC157020.13193975641813557No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAG156370.13139357859574488No Hit
GGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCA155900.13099865001647776No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGT154180.12955337947107468No Hit
GGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGA152450.12809970618994249No Hit
GCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGG149230.12539402528517624No Hit
CGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACG148940.1251503459490327No Hit
GCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCC148680.12493187482007642No Hit
CCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCG148610.12487305566997282No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAG147740.12414201766154218No Hit
GGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCC147560.1239907684184186No Hit
CTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCT146890.12342778512456971No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG146630.12320931399561344No Hit
GCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCGCGGGGAATCCCCCGCGAGGGGGGTCTCCC144600.12150355864260862No Hit
GGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCC142580.1198062060253329No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGG142150.11944488838898212No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGT135570.1139158882792424No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTC134110.11268908886279559No Hit
GTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG132030.11094131983114534No Hit
GCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTA129210.1085717483555426No Hit
GTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCC123880.10409309021193883No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAG123510.1037821889899626No Hit
CGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGC123020.1033704549392373No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT122870.103244413903301No Hit
GTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTCGGGAGGGCGCGCGGGTCGGGGCGG122860.1032360111675719No Hit
CCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTCGGGAGGGCGCGCGGGTCGGGGCGGCG119730.10060595488436744No Hit
GGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACC119220.10017741536218397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)