FastQCFastQC Report
Sat 2 Sep 2023
EGAF00008122745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008122745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42972688
Sequences flagged as poor quality0
Sequence length154
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCCTAATTGTGAGATATATTATCAAAGTCCTTTATCACTTTGTATGG7912741.8413416447209445No Hit
TAGTGGATGGTGGTACAGTCAGAGCCAACCTAGGAGATAACACAGGCCCA4729721.1006339654619697No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3116950.7253327974270541No Hit
CAGCATCTTGAATCTCATACAGACTGCATTCTTGCAGTAAAGCAGGCAAT2037610.47416396200302857No Hit
AGTGGATGGTGGTACAGTCAGAGCCAACCTAGGAGATAACACAGGCCCAA1610780.374838083202987No Hit
AGTCAAGAAGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTT1291640.300572307694599No Hit
ACAGTCAAGAAGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCAC635040.1477775837527315No Hit
GGGGACAGCATCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAG599280.13945601913475833No Hit
AAAACAGTCAAGAAGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTC590410.13739191739646353No Hit
AAACAGTCAAGAAGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCC540450.12576592834965317No Hit
TGTCAAGAAGTTGTTGGTAGTGGATCCAAAGGCACGTTTTATGACAGAAG468910.10911814499479297No Hit
CCTGGGTACATAAGAAGGTGAACAGTTTGCAAGGGGAAGTTTGAATTACT447800.10420572248121876No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)