FastQCFastQC Report
Thu 31 Aug 2023
EGAF00008122755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008122755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17976707
Sequences flagged as poor quality0
Sequence length154
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6822033.794927513698699No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCGTTCTGTGTAGATC4224822.350163464309676Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT528350.2939081112019014No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG427690.23791342874977048No Hit
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCGTTCTGT384880.21409927858311315Illumina Single End PCR Primer 1 (96% over 33bp)
CGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCGTTCTGTGTA334540.1860963746029793Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCGTTCTGTG331130.18419947546566787Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT321320.17874241372460484No Hit
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCGTTCT295590.16442944750670965Illumina Single End PCR Primer 1 (96% over 33bp)
CGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCGTTCTGTGTAG274370.1526252833736457Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTT251850.14009796121169465No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG226430.1259574403699187No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCGTTCTGTGT216540.12045587659630877Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCGTTCTG196640.10938599600026856Illumina Single End PCR Primer 1 (96% over 33bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCGTTCTGTGTAGA187580.10434614081433267Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)